ID   GUN2_PERAE              Reviewed;         130 AA.
AC   P23666;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Endoglucanase 2;
DE            EC=3.2.1.4;
DE   AltName: Full=Abscission cellulase 2;
DE   AltName: Full=Endo-1,4-beta-glucanase 2;
DE   Flags: Fragment;
GN   Name=CEL2;
OS   Persea americana (Avocado).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Magnoliidae; Laurales; Lauraceae; Persea.
OX   NCBI_TaxID=3435;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Hass; TISSUE=Fruit;
RX   PubMed=2259345; DOI=10.1007/bf00315799;
RA   Cass L.G., Kirven K.A., Christoffersen R.E.;
RT   "Isolation and characterization of a cellulase gene family member expressed
RT   during avocado fruit ripening.";
RL   Mol. Gen. Genet. 223:76-86(1990).
CC   -!- FUNCTION: Involved in ripening fruit process.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- DEVELOPMENTAL STAGE: In ripening fruit.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000305}.
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DR   EMBL; X55790; CAA39314.1; -; Genomic_DNA.
DR   PIR; S34493; S34493.
DR   AlphaFoldDB; P23666; -.
DR   SMR; P23666; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0009835; P:fruit ripening; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cellulose degradation; Fruit ripening;
KW   Glycosidase; Hydrolase; Polysaccharide degradation.
FT   CHAIN           <1..130
FT                   /note="Endoglucanase 2"
FT                   /id="PRO_0000184059"
FT   ACT_SITE        47
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        98
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        107
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   NON_TER         1
SQ   SEQUENCE   130 AA;  13892 MW;  A8A9EBC7B0B6F5B6 CRC64;
     ASCGSTTVTA KNLISLAKKQ VDYILGENPA KMSYMVGFGE RYPQHVHHRG SSLPSVHAHP
     NPIPCNAGFQ YLYSSSPNPN ILVGAILGGP DSRDSFSDDR NNYQQSEPAT YINAPLVGAL
     AFFAANPVAN