AMT3_DICDI
ID AMT3_DICDI Reviewed; 431 AA.
AC Q8MXY0; Q55FZ8; Q7Z1M2;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Ammonium transporter 3;
GN Name=amtC; ORFNames=DDB_G0267424;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=AX2;
RA Chen Y., Huang C.-H.;
RT "Cloning and characterization of three ammonium transporter genes in AX2
RT strain of Dictyostelium discoideum.";
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=17951519; DOI=10.1128/ec.00204-07;
RA Yoshino R., Morio T., Yamada Y., Kuwayama H., Sameshima M., Tanaka Y.,
RA Sesaki H., Iijima M.;
RT "Regulation of ammonia homeostasis by the ammonium transporter AmtA in
RT Dictyostelium discoideum.";
RL Eukaryot. Cell 6:2419-2428(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=14686953; DOI=10.1111/j.1432-0436.2003.07109002.x;
RA Follstaedt S.C., Kirsten J.H., Singleton C.K.;
RT "Temporal and spatial expression of ammonium transporter genes during
RT growth and development of Dictyostelium discoideum.";
RL Differentiation 71:557-566(2003).
RN [5]
RP FUNCTION.
RX PubMed=16188250; DOI=10.1016/j.ydbio.2005.08.043;
RA Kirsten J.H., Xiong Y., Dunbar A.J., Rai M., Singleton C.K.;
RT "Ammonium transporter C of Dictyostelium discoideum is required for correct
RT prestalk gene expression and for regulating the choice between slug
RT migration and culmination.";
RL Dev. Biol. 287:146-156(2005).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=19108721; DOI=10.1186/1471-2121-9-71;
RA Kirsten J.H., Xiong Y., Davis C.T., Singleton C.K.;
RT "Subcellular localization of ammonium transporters in Dictyostelium
RT discoideum.";
RL BMC Cell Biol. 9:71-71(2008).
CC -!- FUNCTION: Ammonium transporter that mediates the import of ammonium in
CC prespore cells. Controls ammonium homeostasis during growth and
CC development. Ammonium has been shown to function as a morphogen at
CC multiple steps during the development. May function as an ammonia
CC sensor that relays information concerning ammonia concentrations to the
CC signaling pathway involved in the slug versus culmination choice and
CC regulates prestalk gene expression. {ECO:0000269|PubMed:14686953,
CC ECO:0000269|PubMed:16188250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19108721};
CC Multi-pass membrane protein {ECO:0000269|PubMed:19108721}. Endosome
CC membrane {ECO:0000269|PubMed:19108721}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19108721}. Cytoplasmic vesicle, phagosome membrane
CC {ECO:0000269|PubMed:19108721}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19108721}.
CC -!- DEVELOPMENTAL STAGE: Predominantly found in the prespores of the slug.
CC {ECO:0000269|PubMed:14686953}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAP47147.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF510718; AAP47147.1; ALT_FRAME; mRNA.
DR EMBL; AB089904; BAC07553.1; -; Genomic_DNA.
DR EMBL; AAFI02000003; EAL73164.1; -; Genomic_DNA.
DR RefSeq; XP_647385.1; XM_642293.1.
DR AlphaFoldDB; Q8MXY0; -.
DR SMR; Q8MXY0; -.
DR STRING; 44689.DDB0191130; -.
DR PaxDb; Q8MXY0; -.
DR EnsemblProtists; EAL73164; EAL73164; DDB_G0267424.
DR GeneID; 8616194; -.
DR KEGG; ddi:DDB_G0267424; -.
DR dictyBase; DDB_G0267424; amtC.
DR eggNOG; KOG0682; Eukaryota.
DR HOGENOM; CLU_000445_33_1_1; -.
DR InParanoid; Q8MXY0; -.
DR OMA; IWLSIMS; -.
DR PhylomeDB; Q8MXY0; -.
DR PRO; PR:Q8MXY0; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:dictyBase.
DR GO; GO:0030670; C:phagocytic vesicle membrane; IDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; ISS:dictyBase.
DR GO; GO:0097272; P:ammonium homeostasis; IBA:GO_Central.
DR GO; GO:0072488; P:ammonium transmembrane transport; IMP:dictyBase.
DR GO; GO:0031154; P:culmination involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0050793; P:regulation of developmental process; IMP:dictyBase.
DR GO; GO:0046903; P:secretion; IMP:dictyBase.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR001905; Ammonium_transpt.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR Pfam; PF00909; Ammonium_transp; 1.
DR TIGRFAMs; TIGR00836; amt; 1.
PE 2: Evidence at transcript level;
KW Ammonia transport; Cell membrane; Cytoplasmic vesicle; Endosome; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..431
FT /note="Ammonium transporter 3"
FT /id="PRO_0000365575"
FT TOPO_DOM 1..27
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 49..63
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 85..102
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 103..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..134
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 156..160
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 182..211
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..249
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 271..300
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..333
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 355..357
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 379
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 401..431
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT CONFLICT 353
FT /note="H -> P (in Ref. 1; AAP47147)"
FT /evidence="ECO:0000305"
FT CONFLICT 362
FT /note="I -> L (in Ref. 1; AAP47147)"
FT /evidence="ECO:0000305"
FT CONFLICT 368
FT /note="F -> I (in Ref. 1; AAP47147)"
FT /evidence="ECO:0000305"
FT CONFLICT 379
FT /note="Q -> P (in Ref. 1; AAP47147)"
FT /evidence="ECO:0000305"
FT CONFLICT 413
FT /note="T -> S (in Ref. 1; AAP47147)"
FT /evidence="ECO:0000305"
FT CONFLICT 426
FT /note="S -> R (in Ref. 1; AAP47147)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 431 AA; 46992 MW; 163A1B4D2BA3C2FB CRC64;
MEQFSTSSSE SSDSSSEYSL EFYMDTSWVL DAANLVFFMQ AGFGMLEAGM VRAKNTKSIL
LKNLINTAIC AISYYCVGHS FAYGKVNPNS FVGFGNFFLM DYTHYAYWMI QWAYAATATT
IATGAMAERL QLHCYILFTL VQTILIYPFV AHWIWSQNGW LFDLGIVDFA GGAVIHIVAG
ITGACGSFLL GPRIGRFNQE SGKPKNLPGH SVVLMSLGAM ILWYSWYGYT AGASLGMTRS
RVLPASRVSV VVTLSGATGL ITVLGIGKIF NGHYDLVKGI NGLIAGLVSS TSSCAYIEPW
AAIIIGFIGG IVYWFSSWAL LNWLRLDDPV DSTAIHLFGG CWSLISVAFF ATHGRVRNPD
IILPGGIFYG GGISLLWVQL VGMVLAILWA GFLSGIFFFT MDYFGKLRVD VDTELAGLDN
SNHGGSAYIF D
