AMT3_SCHPO
ID AMT3_SCHPO Reviewed; 517 AA.
AC Q9P7F3;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Ammonium transporter 3;
DE AltName: Full=Ammonium transporter mep3;
GN Name=amt3; Synonyms=mep3; ORFNames=SPAC2E1P3.02c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION.
RC STRAIN=FY7406;
RX PubMed=16999738; DOI=10.1111/j.1365-2443.2006.01014.x;
RA Mitsuzawa H.;
RT "Ammonium transporter genes in the fission yeast Schizosaccharomyces pombe:
RT role in ammonium uptake and a morphological transition.";
RL Genes Cells 11:1183-1195(2006).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Transporter for ammonium to use as a nitrogen source.
CC {ECO:0000269|PubMed:16999738}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:16823372}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
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DR EMBL; CU329670; CAB83006.1; -; Genomic_DNA.
DR RefSeq; NP_593983.1; NM_001019409.2.
DR AlphaFoldDB; Q9P7F3; -.
DR SMR; Q9P7F3; -.
DR BioGRID; 278286; 10.
DR STRING; 4896.SPAC2E1P3.02c.1; -.
DR PaxDb; Q9P7F3; -.
DR EnsemblFungi; SPAC2E1P3.02c.1; SPAC2E1P3.02c.1:pep; SPAC2E1P3.02c.
DR GeneID; 2541795; -.
DR KEGG; spo:SPAC2E1P3.02c; -.
DR PomBase; SPAC2E1P3.02c; amt3.
DR VEuPathDB; FungiDB:SPAC2E1P3.02c; -.
DR eggNOG; KOG0682; Eukaryota.
DR HOGENOM; CLU_000445_33_0_1; -.
DR InParanoid; Q9P7F3; -.
DR OMA; VYCPQAY; -.
DR PhylomeDB; Q9P7F3; -.
DR PRO; PR:Q9P7F3; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:PomBase.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:PomBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IGI:PomBase.
DR GO; GO:0015200; F:methylammonium transmembrane transporter activity; IMP:PomBase.
DR GO; GO:0072488; P:ammonium transmembrane transport; IGI:PomBase.
DR GO; GO:0072489; P:methylammonium transmembrane transport; IMP:PomBase.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR001905; Ammonium_transpt.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR PANTHER; PTHR43029; PTHR43029; 1.
DR Pfam; PF00909; Ammonium_transp; 2.
PE 3: Inferred from homology;
KW Ammonia transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..517
FT /note="Ammonium transporter 3"
FT /id="PRO_0000278389"
FT TOPO_DOM 1..32
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..69
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..118
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 140..148
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..180
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 202..272
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 294..299
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 321..327
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 328..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 349
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 371..384
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 385..405
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 406..432
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 454..517
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 517 AA; 56468 MW; 4185DF8F723ED31E CRC64;
MLNEPNALLR RDANSTIATV TELFPNEYSN ADIAYVLLST VVVFTVTPGI ALYYAGMVRK
NSALSILTQS FLVTAVVFIQ WYLFGYSLAC SSGSSFYGTL WQGGMNHLWL EPYIPGSTIP
AIVYFPFGGL FAVATAQLFA GAMAERGRLI PSLVISFLYI TLVYCPQAYW TWAPNGWLYT
LGALDFAGGG PVHISSGFAA LAYSLCLGRR IVVDEPDRPD SIRSTDNADL PSSSQNNCKA
NWKRWFGFKS VSWKNSFGRG KIAHNPPHDA GMVYIGVVLI WFAWLCFNSG TLLTVNIRTA
YIMTNTLISS SFGALTWAII DYIRYRKFST IGICEGAIAG LVGITPACGF VFPWGAAAGG
IVPALVCNFL HDLNEWIGVD ETLRVFNLHG IGGIVGSIVL GVVAHPDVAA SDGATVIDGG
WAVHHWKQMG YQFAGFTSVA AWSFVITAII CLLVDLVPGL HIRASFEEEL RGMDFVELEE
QLPGQTLESK PMIIYAQEMD EPVTQGSNIK QEKQDEF
