GUN4_ORYSJ
ID GUN4_ORYSJ Reviewed; 503 AA.
AC Q6Z715; A0A0P0VE54; Q0E4D7;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Endoglucanase 4;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 4;
DE AltName: Full=OsCel9F;
DE AltName: Full=OsGLU14;
DE Flags: Precursor;
GN Name=GLU14; OrderedLocusNames=Os02g0123700, LOC_Os02g03120;
GN ORFNames=P0575F10.17;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP INDUCTION.
RX PubMed=17056619; DOI=10.1093/pcp/pcl021;
RA Yoshida K., Imaizumi N., Kaneko S., Kawagoe Y., Tagiri A., Tanaka H.,
RA Nishitani K., Komae K.;
RT "Carbohydrate-binding module of a rice endo-beta-1,4-glycanase, OsCel9A,
RT expressed in auxin-induced lateral root primordia, is post-translationally
RT truncated.";
RL Plant Cell Physiol. 47:1555-1571(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- INDUCTION: By auxin in roots. {ECO:0000269|PubMed:17056619}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; AP004885; BAD07956.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF07651.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS76739.1; -; Genomic_DNA.
DR EMBL; AK106726; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; Q6Z715; -.
DR SMR; Q6Z715; -.
DR STRING; 4530.OS02T0123700-01; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q6Z715; -.
DR PRIDE; Q6Z715; -.
DR EnsemblPlants; Os02t0123700-01; Os02t0123700-01; Os02g0123700.
DR Gramene; Os02t0123700-01; Os02t0123700-01; Os02g0123700.
DR eggNOG; ENOG502QPI6; Eukaryota.
DR HOGENOM; CLU_008926_1_2_1; -.
DR InParanoid; Q6Z715; -.
DR OMA; VACPFYC; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6Z715; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycosidase; Hydrolase; Polysaccharide degradation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..503
FT /note="Endoglucanase 4"
FT /id="PRO_0000249281"
FT ACT_SITE 77
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 418
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 469
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 478
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CONFLICT 39
FT /note="Q -> R (in Ref. 4; AK106726)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 503 AA; 53899 MW; E9EA970F02FD62C0 CRC64;
MSRACLLLVV AAVCLAGREA AAFNYADALD KAILFFEAQR SGKLPPGQRV AWRADSGLSD
GSADGVDLAG GYYDAGDNVK FGLPMAFTVT MLSWSVIEFG DMMPARRSSF LGGIFGGGGV
AQLDNARAAV RWGADYLLKA ATATPDTLYV QVADPYQDHR CWERPEDMDT PRSVYKVTPQ
SPGSDVAGET AAALAAASIV FRVSDPSYSA KLLDAAQLVF DFADKYRGSY SDSLSSVVCP
FYCSHSYHDE LLWAASWLHL ASPEKKDVYL SYIGSNGHAL GAEQDDFTFS WDDKRVATKG
FLQSRADGLQ LYKAHTDNYI CSLVPGANGF QSQYTPGGLL FKEGDSNMQY VTSTAFLLLT
YAKYLSSSAA TVSCGSTAVS PSTLISLAKK QVDYILGANP AGMSYMVGFG ARYPRHVHHR
GASMPSVRDH PARIGCDEGF RYLHSPEPDR NLLAGAVVGG PDAGDAFADG RDNYAQAEPS
TYTNAPLVGA LAFFAGAHKI FTP
