GUN6_ARATH
ID GUN6_ARATH Reviewed; 620 AA.
AC Q42059; Q8H160; Q9C7W3;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Endoglucanase 6;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 6;
DE Flags: Precursor;
GN OrderedLocusNames=At1g64390; ORFNames=F15H21.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 397-514.
RC STRAIN=cv. Columbia; TISSUE=Shoot;
RX PubMed=8580968; DOI=10.1046/j.1365-313x.1996.09010101.x;
RA Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C.,
RA Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R.,
RA Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J.,
RA Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D.,
RA Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P.,
RA Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.;
RT "Further progress towards a catalogue of all Arabidopsis genes: analysis of
RT a set of 5000 non-redundant ESTs.";
RL Plant J. 9:101-124(1996).
RN [5]
RP GENE FAMILY.
RX PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA Libertini E., Li Y., McQueen-Mason S.J.;
RT "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family.";
RL J. Mol. Evol. 58:506-515(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC066689; AAG51703.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34236.1; -; Genomic_DNA.
DR EMBL; AF372940; AAK50080.1; -; mRNA.
DR EMBL; AY143945; AAN28884.1; -; mRNA.
DR EMBL; BT000696; AAN31840.1; -; mRNA.
DR EMBL; Z25957; CAA81116.1; -; mRNA.
DR PIR; A96668; A96668.
DR RefSeq; NP_176621.1; NM_105114.3.
DR AlphaFoldDB; Q42059; -.
DR SMR; Q42059; -.
DR STRING; 3702.AT1G64390.1; -.
DR CAZy; CBM49; Carbohydrate-Binding Module Family 49.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR iPTMnet; Q42059; -.
DR PaxDb; Q42059; -.
DR PRIDE; Q42059; -.
DR ProteomicsDB; 247247; -.
DR EnsemblPlants; AT1G64390.1; AT1G64390.1; AT1G64390.
DR GeneID; 842747; -.
DR Gramene; AT1G64390.1; AT1G64390.1; AT1G64390.
DR KEGG; ath:AT1G64390; -.
DR Araport; AT1G64390; -.
DR TAIR; locus:2014205; AT1G64390.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR HOGENOM; CLU_008926_1_4_1; -.
DR InParanoid; Q42059; -.
DR OMA; ASMIFRT; -.
DR OrthoDB; 1195424at2759; -.
DR PhylomeDB; Q42059; -.
DR BioCyc; ARA:AT1G64390-MON; -.
DR PRO; PR:Q42059; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q42059; baseline and differential.
DR Genevisible; Q42059; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR019028; CBM_49.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF09478; CBM49; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SMART; SM01063; CBM49; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..620
FT /note="Endoglucanase 6"
FT /id="PRO_0000249259"
FT ACT_SITE 78
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 411
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 463
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 472
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CARBOHYD 554
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 564
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 196
FT /note="R -> K (in Ref. 3; AAN31840)"
FT /evidence="ECO:0000305"
FT CONFLICT 442
FT /note="S -> V (in Ref. 4; CAA81116)"
FT /evidence="ECO:0000305"
FT CONFLICT 511..514
FT /note="MPIR -> NAYS (in Ref. 4; CAA81116)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 620 AA; 68592 MW; 57847AE1990D427F CRC64;
MEKFAPVAAL LLLLLCFPVA FSGHDYGQAL SKSLLFFEAQ RSGVLPRNQR VTWRSHSGLT
DGKSSGVNLV GGYYDAGDNV KFGLPMAFTV TMMAWSVIEY GNQLQANGEL GNSIDAIKWG
TDYFIKAHPE PNVLYGEVGD GNTDHYCWQR PEEMTTDRKA YRIDPSNPGS DLAGETAAAM
AAASIVFRRS NPVYSRLLLT HAYQLFDFAD KYRGKYDSSI TVAQKYYRSV SGYNDELLWA
AAWLYQASNN QFYLDYLGRN GDAMGGTGWS MTEFGWDVKY AGVQTLVAKF LMQGKAGRHA
PVFRKYQEKA DSFMCSLLGK SSRNIQKTPG GLIFRQRWNN MQFVTSASFL TTVYSDYLTS
SRSNLRCAAG NVAPSQLLSF AKSQVDYILG DNPRATSYMV GYGNNFPQRV HHRGSSIVSV
KVDRTFVTCR GGYATWFSRK GSDPNLLTGA IVGGPDAYDN FADRRDNYEQ TEPATYNNAP
LLGVLARLSS GHSGYSQFLP VVPAPVVRRP MPIRRPKVTT PVRASGPVAI VQKITSSWVS
KGRTYYRYST TVINKSSRPL KSLNLSIKNL YGPIWGLSRS GNSFGLPSWM HSLPSGKSLE
FVYIHSTTPA NVAVSSYTLA
