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GUN6_DICDI
ID   GUN6_DICDI              Reviewed;         705 AA.
AC   P22699; Q55BI5;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 129.
DE   RecName: Full=Endoglucanase;
DE            EC=3.2.1.4;
DE   AltName: Full=Cellulase;
DE   AltName: Full=Endo-1,4-beta-glucanase;
DE   AltName: Full=Spore germination protein 270-6;
DE   Flags: Precursor;
GN   Name=celA; ORFNames=DDB_G0271134;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1976383; DOI=10.1021/bi00483a015;
RA   Giorda R., Ohmachi T., Shaw D.R., Ennis H.L.;
RT   "A shared internal threonine-glutamic acid-threonine-proline repeat defines
RT   a family of Dictyostelium discoideum spore germination specific proteins.";
RL   Biochemistry 29:7264-7269(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [4]
RP   FUNCTION.
RX   PubMed=1869562; DOI=10.1016/s0021-9258(18)98634-5;
RA   Blume J.E., Ennis H.L.;
RT   "A Dictyostelium discoideum cellulase is a member of a spore germination-
RT   specific gene family.";
RL   J. Biol. Chem. 266:15432-15437(1991).
CC   -!- FUNCTION: May digest the spore cell wall during germination, to release
CC       the enclosed amoeba. {ECO:0000269|PubMed:1869562}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- DEVELOPMENTAL STAGE: Found predominantly during early stages of spore
CC       germination.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR   EMBL; M33861; AAA52077.1; -; Genomic_DNA.
DR   EMBL; AAFI02000006; EAL71697.1; -; Genomic_DNA.
DR   PIR; A35621; A35621.
DR   RefSeq; XP_645627.1; XM_640535.1.
DR   AlphaFoldDB; P22699; -.
DR   SMR; P22699; -.
DR   STRING; 44689.DDB0215351; -.
DR   CAZy; CBM8; Carbohydrate-Binding Module Family 8.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PaxDb; P22699; -.
DR   PRIDE; P22699; -.
DR   EnsemblProtists; EAL71697; EAL71697; DDB_G0271134.
DR   GeneID; 8617819; -.
DR   KEGG; ddi:DDB_G0271134; -.
DR   dictyBase; DDB_G0271134; celA.
DR   eggNOG; ENOG502QRF6; Eukaryota.
DR   HOGENOM; CLU_008926_4_0_1; -.
DR   InParanoid; P22699; -.
DR   OMA; YPRQAHH; -.
DR   PhylomeDB; P22699; -.
DR   PRO; PR:P22699; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IDA:dictyBase.
DR   GO; GO:0008810; F:cellulase activity; IDA:dictyBase.
DR   GO; GO:0030248; F:cellulose binding; IDA:dictyBase.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0009847; P:spore germination; IEP:dictyBase.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cellulose degradation; Glycoprotein; Glycosidase;
KW   Hydrolase; Polysaccharide degradation; Reference proteome; Repeat; Signal;
KW   Sporulation.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..705
FT                   /note="Endoglucanase"
FT                   /id="PRO_0000007953"
FT   REGION          455..556
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          463..552
FT                   /note="Pro/Thr repeats ('hinge') (Pro/Thr box)"
FT   ACT_SITE        85
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        390
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        428
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        437
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   CARBOHYD        75
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   705 AA;  75911 MW;  F0457BDC07576A15 CRC64;
     MKILKNCILL IIFGLLSTQL INADTDYCSL LENALMFYKM NRAGRLPDND IPWRGNSALN
     DASPNSAKDA NGDGNLSGGY FDAGDGVKFG LPMAYSMTML GWSFIEYESN IAQCGLTSLY
     LDTIKYGTDW LIAAHTADNE FAGQVGDGNV DHSWWGPPED MTMARPTYML TTEAPGTEIA
     MEAASALAAA SIAFKSSNPT YAATCLAHAK TLHNFGYTYR GVYSDSITNA QAFYNSWSGY
     KDDLVWGSIW LYKATQDSDY LTKAVADYAS GGVGGMAQGN SHDWDNKAPG CCLLLSKLVP
     TTSTYKTDFE GWLNYWLPGG GVTYTPGGLA WIRQWGPARY AATAAFLGSL AGTEKGTDFT
     QKQVDYLIGN NPNQQSFVVG MGPNYPINPH HRAAHHSTTN DINNPVNNLY LLKGALVGGP
     GSNDEYTDDR TDYISNEVAT DYNAGFVGAL ASLVNPSSTS VPTTTPTVTE TPTETPTETP
     TETPTETPTE TPTETPTETP TETPTETPTE TPTETPTETP TETPTETPTE TPTETPTETV
     TPTPTVTPTE TPSSGESLSI YKSGLKNDFQ DWSWGEHSLT DTTNVESGET NSISFTPKAY
     GAVFLGCFEC IDTDTYNNIE FDINGGSSGA QLLRITVVKN SKSVGSKLIT DLNGGTPIEA
     NSWTKIKASF IDDFKVSGKV DGIWIQDIKG DTQSTVYISN IIATA
 
 
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