GUN6_DICDI
ID GUN6_DICDI Reviewed; 705 AA.
AC P22699; Q55BI5;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=Endoglucanase;
DE EC=3.2.1.4;
DE AltName: Full=Cellulase;
DE AltName: Full=Endo-1,4-beta-glucanase;
DE AltName: Full=Spore germination protein 270-6;
DE Flags: Precursor;
GN Name=celA; ORFNames=DDB_G0271134;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1976383; DOI=10.1021/bi00483a015;
RA Giorda R., Ohmachi T., Shaw D.R., Ennis H.L.;
RT "A shared internal threonine-glutamic acid-threonine-proline repeat defines
RT a family of Dictyostelium discoideum spore germination specific proteins.";
RL Biochemistry 29:7264-7269(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP FUNCTION.
RX PubMed=1869562; DOI=10.1016/s0021-9258(18)98634-5;
RA Blume J.E., Ennis H.L.;
RT "A Dictyostelium discoideum cellulase is a member of a spore germination-
RT specific gene family.";
RL J. Biol. Chem. 266:15432-15437(1991).
CC -!- FUNCTION: May digest the spore cell wall during germination, to release
CC the enclosed amoeba. {ECO:0000269|PubMed:1869562}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- DEVELOPMENTAL STAGE: Found predominantly during early stages of spore
CC germination.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; M33861; AAA52077.1; -; Genomic_DNA.
DR EMBL; AAFI02000006; EAL71697.1; -; Genomic_DNA.
DR PIR; A35621; A35621.
DR RefSeq; XP_645627.1; XM_640535.1.
DR AlphaFoldDB; P22699; -.
DR SMR; P22699; -.
DR STRING; 44689.DDB0215351; -.
DR CAZy; CBM8; Carbohydrate-Binding Module Family 8.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; P22699; -.
DR PRIDE; P22699; -.
DR EnsemblProtists; EAL71697; EAL71697; DDB_G0271134.
DR GeneID; 8617819; -.
DR KEGG; ddi:DDB_G0271134; -.
DR dictyBase; DDB_G0271134; celA.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR HOGENOM; CLU_008926_4_0_1; -.
DR InParanoid; P22699; -.
DR OMA; YPRQAHH; -.
DR PhylomeDB; P22699; -.
DR PRO; PR:P22699; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005576; C:extracellular region; IDA:dictyBase.
DR GO; GO:0008810; F:cellulase activity; IDA:dictyBase.
DR GO; GO:0030248; F:cellulose binding; IDA:dictyBase.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0009847; P:spore germination; IEP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cellulose degradation; Glycoprotein; Glycosidase;
KW Hydrolase; Polysaccharide degradation; Reference proteome; Repeat; Signal;
KW Sporulation.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..705
FT /note="Endoglucanase"
FT /id="PRO_0000007953"
FT REGION 455..556
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 463..552
FT /note="Pro/Thr repeats ('hinge') (Pro/Thr box)"
FT ACT_SITE 85
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 390
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 428
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 437
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CARBOHYD 75
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 705 AA; 75911 MW; F0457BDC07576A15 CRC64;
MKILKNCILL IIFGLLSTQL INADTDYCSL LENALMFYKM NRAGRLPDND IPWRGNSALN
DASPNSAKDA NGDGNLSGGY FDAGDGVKFG LPMAYSMTML GWSFIEYESN IAQCGLTSLY
LDTIKYGTDW LIAAHTADNE FAGQVGDGNV DHSWWGPPED MTMARPTYML TTEAPGTEIA
MEAASALAAA SIAFKSSNPT YAATCLAHAK TLHNFGYTYR GVYSDSITNA QAFYNSWSGY
KDDLVWGSIW LYKATQDSDY LTKAVADYAS GGVGGMAQGN SHDWDNKAPG CCLLLSKLVP
TTSTYKTDFE GWLNYWLPGG GVTYTPGGLA WIRQWGPARY AATAAFLGSL AGTEKGTDFT
QKQVDYLIGN NPNQQSFVVG MGPNYPINPH HRAAHHSTTN DINNPVNNLY LLKGALVGGP
GSNDEYTDDR TDYISNEVAT DYNAGFVGAL ASLVNPSSTS VPTTTPTVTE TPTETPTETP
TETPTETPTE TPTETPTETP TETPTETPTE TPTETPTETP TETPTETPTE TPTETPTETV
TPTPTVTPTE TPSSGESLSI YKSGLKNDFQ DWSWGEHSLT DTTNVESGET NSISFTPKAY
GAVFLGCFEC IDTDTYNNIE FDINGGSSGA QLLRITVVKN SKSVGSKLIT DLNGGTPIEA
NSWTKIKASF IDDFKVSGKV DGIWIQDIKG DTQSTVYISN IIATA