GUN6_ORYSJ
ID GUN6_ORYSJ Reviewed; 508 AA.
AC Q6Z2J3; A0A0N7KG18; Q0DXU1;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Endoglucanase 6;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 6;
DE AltName: Full=OsCel9E;
DE Flags: Precursor;
GN OrderedLocusNames=Os02g0733300, LOC_Os02g50040; ORFNames=P0643A10.32;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP INDUCTION.
RX PubMed=17056619; DOI=10.1093/pcp/pcl021;
RA Yoshida K., Imaizumi N., Kaneko S., Kawagoe Y., Tagiri A., Tanaka H.,
RA Nishitani K., Komae K.;
RT "Carbohydrate-binding module of a rice endo-beta-1,4-glycanase, OsCel9A,
RT expressed in auxin-induced lateral root primordia, is post-translationally
RT truncated.";
RL Plant Cell Physiol. 47:1555-1571(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- INDUCTION: By auxin in roots. {ECO:0000269|PubMed:17056619}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; AP005319; BAD16147.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF09947.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS80777.1; -; Genomic_DNA.
DR EMBL; AK101108; BAG94917.1; -; mRNA.
DR EMBL; AK105446; BAG97254.1; -; mRNA.
DR RefSeq; XP_015625116.1; XM_015769630.1.
DR AlphaFoldDB; Q6Z2J3; -.
DR SMR; Q6Z2J3; -.
DR STRING; 4530.OS02T0733300-01; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q6Z2J3; -.
DR PRIDE; Q6Z2J3; -.
DR EnsemblPlants; Os02t0733300-01; Os02t0733300-01; Os02g0733300.
DR GeneID; 4330637; -.
DR Gramene; Os02t0733300-01; Os02t0733300-01; Os02g0733300.
DR KEGG; osa:4330637; -.
DR eggNOG; ENOG502QR9R; Eukaryota.
DR HOGENOM; CLU_008926_1_2_1; -.
DR InParanoid; Q6Z2J3; -.
DR OMA; YLSHANH; -.
DR OrthoDB; 1195424at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6Z2J3; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycosidase; Hydrolase; Polysaccharide degradation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT CHAIN 34..508
FT /note="Endoglucanase 6"
FT /id="PRO_0000249283"
FT ACT_SITE 89
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 419
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 471
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 480
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 508 AA; 55331 MW; 124DF097CB76241C CRC64;
MLAASLRVEA VAVVAAAVLV LLLSPAAVVV VAGQHDYGDA LHKSILFFEG QRSGRLPPDQ
RLRWRRDSGL HDGAAASVDL TGGYYDAGDN VKFGFPMAFT ATLMSWGLID FGRSFGPHKE
EARKAVRWAT DYLMKATAKP NTVYVQVGDA FRDHSCWERP EDMDTPRTVY KVDPSHPGSD
VAAETAAALA AGSIVFRDAD PAYSKRLLDR AIAVFEFADK YRGPYSSSLH DAVCPCYCDF
SGYKDELLWG AAWLHKASRR REYREYIKKN EVVLGASESI NEFGWDNKHA GINVLISKEV
LMGKDEYFQS FRVNADNFMC SLLPGISNHP QIQYSPGGLL FKVGGSNMQH VTSLSFLLLA
YSNYLSHAGA RVSCGAGGSA SPTQLRRVAK RQVDYILGDN PLRMSYMVGY GARFPRRIHH
RGSSLPSVAA HPARIGCKGG AAYYASAAPN PNLLVGAVVG GPSDATDAFP DARAVFQQSE
PTTYINAPLM GLLAYFSAHP NPAEWADD
