GUN7_ORYSJ
ID GUN7_ORYSJ Reviewed; 531 AA.
AC Q6Z5P2; Q0DXR2;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Endoglucanase 7;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 7;
DE AltName: Full=OsGLU10;
DE Flags: Precursor;
GN Name=GLU10; OrderedLocusNames=Os02g0738600, LOC_Os02g50490;
GN ORFNames=P0684F11.11;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=16463105; DOI=10.1007/s11103-005-2972-x;
RA Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S.,
RA Chen S.-Y.;
RT "OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice,
RT affects plant internode elongation.";
RL Plant Mol. Biol. 60:137-151(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:16463105}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF09976.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AP005112; BAD16040.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF09976.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK099698; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015622868.1; XM_015767382.1.
DR AlphaFoldDB; Q6Z5P2; -.
DR SMR; Q6Z5P2; -.
DR STRING; 4530.OS02T0738600-01; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q6Z5P2; -.
DR PRIDE; Q6Z5P2; -.
DR GeneID; 4330667; -.
DR KEGG; osa:4330667; -.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR HOGENOM; CLU_008926_1_4_1; -.
DR InParanoid; Q6Z5P2; -.
DR OrthoDB; 1195424at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6Z5P2; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycosidase; Hydrolase; Polysaccharide degradation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..531
FT /note="Endoglucanase 7"
FT /id="PRO_0000249284"
FT ACT_SITE 89
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 430
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 482
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 491
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT CONFLICT 127
FT /note="A -> V (in Ref. 4; AK099698)"
FT /evidence="ECO:0000305"
FT CONFLICT 380
FT /note="D -> G (in Ref. 4; AK099698)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 531 AA; 58598 MW; 6E87230EDEA34F70 CRC64;
MRGRALVLVA ALLLQLLLLA AAGGAGAAAT ERKAHNYEDA LRKSLLYFEA QRSGRLPHNQ
RVAWRDHSGL TDGLEQGVDL VGGYYDAGDH VKFGLPMAFT VTMLSWSMIE YGDDVEAAGE
LGHALEAIKW GTDYFIKAHT KPNELWAEVG DGDTDHYCWQ RPEDMTTSRQ AYKVDRERPG
SDVAGETAAA MAAASIVFRK SNPHYASLLL HHAQQLFEFA DKYRGKYDSS IAEVKSYYAS
VSGYKDELLW AALWLHRATG KAHYLDYVVD NADCFGGTGW AITEFSWDVK YAGVQILAAR
LLLRGEHEER HRSTLEQYRA KAEHYVCGCL GRNADGGADA NVERSPGGML YVRQWNNMQY
VTNAAFLLAA YADYLGDDAD GAVSCAGGET AGAGEVAALA RAQVDYVLGT NPRGISYLVG
YGAKYPNRVH HRAASIVPYK HSKEFIGCTQ GFDHWFGRRS SNPNVLVGAI VGGPDRRDRF
RDNRENYMQT EACTYNTAPM VGMFAKLNRM ARQEREQEEV AAPARSTAAD V
