GUN8_ORYSJ
ID GUN8_ORYSJ Reviewed; 501 AA.
AC Q6K7G9; A0A0P0VQD5; Q0DX27;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Endoglucanase 8;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 8;
DE Flags: Precursor;
GN OrderedLocusNames=Os02g0778600, LOC_Os02g53820;
GN ORFNames=OJ1293_A01.6, OsJ_008299;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; AP004846; BAD19513.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF10211.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS81192.1; -; Genomic_DNA.
DR EMBL; CM000139; EAZ24816.1; -; Genomic_DNA.
DR EMBL; AK100190; BAG94487.1; -; mRNA.
DR EMBL; AK106971; BAG97900.1; -; mRNA.
DR RefSeq; XP_015624767.1; XM_015769281.1.
DR AlphaFoldDB; Q6K7G9; -.
DR SMR; Q6K7G9; -.
DR STRING; 4530.OS02T0778600-01; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q6K7G9; -.
DR PRIDE; Q6K7G9; -.
DR EnsemblPlants; Os02t0778600-01; Os02t0778600-01; Os02g0778600.
DR GeneID; 4330912; -.
DR Gramene; Os02t0778600-01; Os02t0778600-01; Os02g0778600.
DR KEGG; osa:4330912; -.
DR eggNOG; ENOG502QQ06; Eukaryota.
DR HOGENOM; CLU_008926_1_2_1; -.
DR InParanoid; Q6K7G9; -.
DR OMA; PSWRGDS; -.
DR OrthoDB; 1195424at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..501
FT /note="Endoglucanase 8"
FT /id="PRO_0000249285"
FT ACT_SITE 95
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 414
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 465
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 474
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT CARBOHYD 298
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 462
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 469
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 501 AA; 54541 MW; 196C62B858CFCC08 CRC64;
MKPRSSRDGH NAAAAAALLL AALVLSGDVL PAVVAGGAPS FNYKDALTKS IMFLEAQRSG
KLPPTNRIKW RGDSGMEDGK LANVDLTGGY YDAGDNVKYG LPLAFTVTTL AWTAMAFEKE
LKAARELENV HAAIRWGTDY FLKAATKKDH LWVQVGDPNA DHQCWVRPEN MPTPRTLYQI
NDKTPGSEIA AETAAAMTAS SMVFRKDKPY SRRLLNKAKL LFQFAKTHQG TYDGECPFYC
SYSGYNDELL WAATWLYLAT KRQVYADFIG HEAISSSVAE FSWDLKFPGA QVLLAELNMT
SSGGLQSFKS QADNFVCAVL PDTPFHQVSI TPGGMIHLRD GANSQYVTST AFLFVAYSDI
LRRINQPVMC GAQAVQPARL LQFAKQQIDY LLGANPRGRS YVVGFGVNPP TQPHHRGAST
PVLPPGYQVN CGMSFSEWFT PDRPNPNELT GAIMGGPDGG DNFSDKRGNS SCTEPCTYIN
SLSIGPLAAL AIRGPNLIAT Q
