ID   GUNA_ASPKW              Reviewed;         239 AA.
AC   Q12679; G7XJV2;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 2.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Endoglucanase A;
DE            EC=3.2.1.4;
DE   AltName: Full=CMCase-I;
DE   AltName: Full=Carboxymethylcellulase;
DE   AltName: Full=Cellulase A;
DE   AltName: Full=Endo-1,4-beta-glucanase A;
DE   Flags: Precursor;
GN   Name=cekA; ORFNames=AKAW_05147;
OS   Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus
OS   awamori var. kawachi).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=1033177;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=NBRC 4308;
RX   PubMed=7586029; DOI=10.1007/bf00311212;
RA   Sakamoto S., Tamura G., Ito K., Ishikawa T., Iwano K., Nishiya N.;
RT   "Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its
RT   expression in Saccharomyces cerevisiae.";
RL   Curr. Genet. 27:435-439(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 4308;
RX   PubMed=22045919; DOI=10.1128/ec.05224-11;
RA   Futagami T., Mori K., Yamashita A., Wada S., Kajiwara Y., Takashita H.,
RA   Omori T., Takegawa K., Tashiro K., Kuhara S., Goto M.;
RT   "Genome sequence of the white koji mold Aspergillus kawachii IFO 4308, used
RT   for brewing the Japanese distilled spirit shochu.";
RL   Eukaryot. Cell 10:1586-1587(2011).
CC   -!- FUNCTION: Has carboxylmethylcellulase activity.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 12 (cellulase H) family.
CC       {ECO:0000305}.
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DR   EMBL; D12901; BAA02297.1; -; mRNA.
DR   EMBL; DF126458; GAA87033.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q12679; -.
DR   SMR; Q12679; -.
DR   STRING; 1033177.Q12679; -.
DR   CAZy; GH12; Glycoside Hydrolase Family 12.
DR   VEuPathDB; FungiDB:AKAW_05147; -.
DR   eggNOG; ENOG502SH4Y; Eukaryota.
DR   InParanoid; Q12679; -.
DR   Proteomes; UP000006812; Unassembled WGS sequence.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.180; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR013319; GH11/12.
DR   InterPro; IPR002594; GH12.
DR   PANTHER; PTHR34002; PTHR34002; 1.
DR   Pfam; PF01670; Glyco_hydro_12; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cellulose degradation; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Signal.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..239
FT                   /note="Endoglucanase A"
FT                   /id="PRO_0000008019"
FT   CONFLICT        217
FT                   /note="F -> C (in Ref. 1; BAA02297)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   239 AA;  25813 MW;  E3B0304A13827066 CRC64;
     MKLSMTLSLF AATAMGQTMC SQYDSASSPP YSVNQNLWGE YQGTGSQCVY VDKLSSSGAS
     WHTKWTWSGG EGTVKSYSNS GLTFDKKLVS DVSSIPTSVT WSQDDTNVQA DVSYDLFTAA
     NADHATSSGD YELMIWLARY GSVQPIGKQI ATATVGGKSW EVWYGTSTQA GAEQKTYSFV
     AGSPINSWSG DIKDFFNYLT QNQGFPASSQ HLITLQFGTE PFTGGPATFT VDNWTASVN