GUNA_FIBSU
ID GUNA_FIBSU Reviewed; 453 AA.
AC P23665; P23664;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Endoglucanase A;
DE EC=3.2.1.4;
DE AltName: Full=Cellulase;
DE AltName: Full=Endo-1,4-beta-glucanase;
DE Flags: Precursor;
GN Name=endA;
OS Fibrobacter succinogenes (Bacteroides succinogenes).
OC Bacteria; Fibrobacteres; Fibrobacterales; Fibrobacteraceae; Fibrobacter.
OX NCBI_TaxID=833;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AR1;
RX PubMed=1708767; DOI=10.1128/jb.173.10.3265-3268.1991;
RA Cavicchioli R., East P.D., Watson K.;
RT "endAFS, a novel family E endoglucanase gene from Fibrobacter succinogenes
RT AR1.";
RL J. Bacteriol. 173:3265-3268(1991).
RN [2]
RP RIBOSOMAL FRAMESHIFT.
RC STRAIN=AR1;
RX PubMed=2027774; DOI=10.1093/nar/19.7.1661;
RA Cavicchioli R., Watson K.;
RT "The involvement of transcriptional read-through from internal promoters in
RT the expression of a novel endoglucanase gene FSendA, from Fibrobacter
RT succinogenes AR1.";
RL Nucleic Acids Res. 19:1661-1669(1991).
RN [3]
RP SUBSTRATES, SUBUNIT, SUBCELLULAR LOCATION, AND CHARACTERIZATION.
RC STRAIN=AR1;
RX PubMed=2014986; DOI=10.1128/aem.57.2.359-365.1991;
RA Cavicchioli R., Watson K.;
RT "Molecular cloning, expression, and characterization of endoglucanase genes
RT from Fibrobacter succinogenes AR1.";
RL Appl. Environ. Microbiol. 57:359-365(1991).
CC -!- FUNCTION: High levels of endoglucanase activity detected on acid-
CC swollen cellulose, ball-milled cellulose, and carboxymethyl cellulose;
CC moderate levels detected on filter paper, phosphoric acid-swollen
CC cellulose, lichenan, and xylan.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 5.0.;
CC Temperature dependence:
CC Optimum temperature is 37-39 degrees Celsius.;
CC -!- SUBUNIT: Monomer. {ECO:0000305|PubMed:2014986}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:2014986}. Note=80%
CC periplasmic, 16% extracellular upon overexpression in E.coli.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC -!- CAUTION: Expression of endA requires a ribosomal frameshift to allow
CC contiguous translation of a 453 amino acid protein; this is suggested
CC to take place at position 127 but could in fact occur anywhere between
CC positions 82 and 127. {ECO:0000305}.
CC -!- CAUTION: On the basis of sequence similarities the C-terminal part of
CC this sequence seems to be incorrect. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA24895.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; M58520; AAA24894.1; -; Genomic_DNA.
DR EMBL; M58520; AAA24895.1; ALT_FRAME; Genomic_DNA.
DR PIR; A39416; A39416.
DR AlphaFoldDB; P23665; -.
DR SMR; P23665; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Cellulose degradation; Glycosidase; Hydrolase;
KW Periplasm; Polysaccharide degradation; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..453
FT /note="Endoglucanase A"
FT /id="PRO_0000007954"
FT REGION 115..126
FT /note="Linker ('hinge') (Pro-Thr box)"
FT ACT_SITE 82
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 417
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
SQ SEQUENCE 453 AA; 50042 MW; 7E861B76070D1186 CRC64;
MNCRKYLLSG LAVFGLAATS AVAALSTDDY VEAAWMTTRF FGAQRSGQGP NWILDGTSNP
TSFTKDSYNG KDVSGGWFDC GDHVMYGQSQ GYASYVLALA YAEFTEVSTT FILVTTPTTR
KPTTTPMKSG KPNKVRDLLE ELRYEADFWV KAAIDGNNFV TVKGDGNADH QKWVTAGAMS
KLGSGEGGEP RCITGNANDG FTSGLAAAML AVMARVDPDT ANQAKYLKAA KTAYSYAKSH
KGVTNSQGFY ESSWWDGRWE DGPFLAELEL YRTTGENSYK TAAIDRYDNL KFSLGEGTHF
MYSNVVPLSA VMAEAVFEET PHGMRKEAIG VLDLIYEEKA KDKIFQNPNG MGSGKFPVRV
PSGGAFLYAL SDKFNNTNEH MEMIEKNVSY LLGDNGSKKS YVVGFSKNGA NAPSRPHHRG
YYANEKRWRR SRRCSESSRK EQALGRYDCW RLY