GUNA_GLOTR
ID GUNA_GLOTR Reviewed; 56 AA.
AC P84194;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 02-JUN-2021, entry version 31.
DE RecName: Full=Endoglucanase Cel5A;
DE EC=3.2.1.4;
DE AltName: Full=Cellulase;
DE AltName: Full=Endo-1,4-beta-glucanase;
DE Flags: Fragments;
OS Gloeophyllum trabeum (Brown rot fungus) (Agaricus trabeus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Gloeophyllales; Gloeophyllaceae; Gloeophyllum.
OX NCBI_TaxID=104355;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 11539 / Madison 617 {ECO:0000269|PubMed:15870328};
RX PubMed=15870328; DOI=10.1128/aem.71.5.2412-2417.2005;
RA Cohen R., Suzuki M.R., Hammel K.E.;
RT "Processive endoglucanase active in crystalline cellulose hydrolysis by the
RT brown rot basidiomycete Gloeophyllum trabeum.";
RL Appl. Environ. Microbiol. 71:2412-2417(2005).
CC -!- FUNCTION: Has avicelase and carboxymethylcellulase activity.
CC {ECO:0000269|PubMed:15870328}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000269|PubMed:15870328};
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space
CC {ECO:0000269|PubMed:15870328}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000255}.
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DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IDA:UniProtKB.
DR GO; GO:0030245; P:cellulose catabolic process; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Cellulose degradation; Direct protein sequencing;
KW Glycosidase; Hydrolase; Polysaccharide degradation; Secreted.
FT CHAIN 1..>56
FT /note="Endoglucanase Cel5A"
FT /id="PRO_0000184048"
FT REGION 36..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 45
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:P07982"
FT NON_CONS 18..19
FT /evidence="ECO:0000303|PubMed:15870328"
FT NON_CONS 30..31
FT /evidence="ECO:0000303|PubMed:15870328"
FT NON_CONS 39..40
FT /evidence="ECO:0000303|PubMed:15870328"
FT NON_TER 56
FT /evidence="ECO:0000303|PubMed:15870328"
SQ SEQUENCE 56 AA; 5622 MW; C3464C0325FAE6B9 CRC64;
VTGPAPLKFA GVNIAGFDYD ALVQAXLNXG YLDSDNSGTL AINTETGGGN TASXVE