GUNA_NOVHA
ID GUNA_NOVHA Reviewed; 342 AA.
AC P37696;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Probable endoglucanase;
DE EC=3.2.1.4;
DE AltName: Full=Cellulase;
DE AltName: Full=Endo-1,4-beta-glucanase;
DE Flags: Precursor;
GN Name=cmcAX;
OS Novacetimonas hansenii (Komagataeibacter hansenii).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Acetobacteraceae; Novacetimonas.
OX NCBI_TaxID=436;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 21-34.
RC STRAIN=ATCC 23769 / NCIMB 8246;
RX PubMed=8300521; DOI=10.1128/jb.176.3.665-672.1994;
RA Standal R., Iversen T.-G., Coucheron D.H., Fjaervik E., Blatny J.M.,
RA Valla S.;
RT "A new gene required for cellulose production and a gene encoding
RT cellulolytic activity in Acetobacter xylinum are colocalized with the bcs
RT operon.";
RL J. Bacteriol. 176:665-672(1994).
CC -!- FUNCTION: Enzyme capable of hydrolyzing carboxy-methyl-cellulose (CMC).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 8 (cellulase D) family.
CC {ECO:0000305}.
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DR EMBL; M96060; AAA16969.1; -; Unassigned_DNA.
DR PIR; A36963; A36963.
DR PDB; 1WZZ; X-ray; 1.65 A; A=21-342.
DR PDBsum; 1WZZ; -.
DR AlphaFoldDB; P37696; -.
DR SMR; P37696; -.
DR CAZy; GH8; Glycoside Hydrolase Family 8.
DR EvolutionaryTrace; P37696; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR002037; Glyco_hydro_8.
DR InterPro; IPR019834; Glyco_hydro_8_CS.
DR Pfam; PF01270; Glyco_hydro_8; 1.
DR PRINTS; PR00735; GLHYDRLASE8.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS00812; GLYCOSYL_HYDROL_F8; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Cellulose degradation;
KW Direct protein sequencing; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000269|PubMed:8300521"
FT CHAIN 21..342
FT /note="Probable endoglucanase"
FT /id="PRO_0000007930"
FT ACT_SITE 57
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 114
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10058"
FT HELIX 26..38
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 48..51
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 53..55
FT /evidence="ECO:0007829|PDB:1WZZ"
FT HELIX 56..69
FT /evidence="ECO:0007829|PDB:1WZZ"
FT HELIX 72..85
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 89..92
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 96..99
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 102..106
FT /evidence="ECO:0007829|PDB:1WZZ"
FT HELIX 113..130
FT /evidence="ECO:0007829|PDB:1WZZ"
FT HELIX 133..150
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 151..154
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 157..160
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 164..166
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 170..175
FT /evidence="ECO:0007829|PDB:1WZZ"
FT HELIX 177..179
FT /evidence="ECO:0007829|PDB:1WZZ"
FT HELIX 182..192
FT /evidence="ECO:0007829|PDB:1WZZ"
FT HELIX 196..210
FT /evidence="ECO:0007829|PDB:1WZZ"
FT TURN 214..217
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 221..226
FT /evidence="ECO:0007829|PDB:1WZZ"
FT TURN 227..229
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 232..234
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 240..242
FT /evidence="ECO:0007829|PDB:1WZZ"
FT TURN 244..247
FT /evidence="ECO:0007829|PDB:1WZZ"
FT HELIX 248..255
FT /evidence="ECO:0007829|PDB:1WZZ"
FT HELIX 261..274
FT /evidence="ECO:0007829|PDB:1WZZ"
FT TURN 285..287
FT /evidence="ECO:0007829|PDB:1WZZ"
FT STRAND 292..294
FT /evidence="ECO:0007829|PDB:1WZZ"
FT HELIX 297..305
FT /evidence="ECO:0007829|PDB:1WZZ"
FT HELIX 317..319
FT /evidence="ECO:0007829|PDB:1WZZ"
FT HELIX 323..339
FT /evidence="ECO:0007829|PDB:1WZZ"
SQ SEQUENCE 342 AA; 37443 MW; 85FF9BDF8B979F81 CRC64;
MSVMAAMGGA QVLSSTGAFA DPAPDAVAQQ WAIFRAKYLR PSGRVVDTGN GGESHSEGQG
YGMLFAASAG DLASFQSMWM WARTNLQHTN DKLFSWRFLK GHQPPVPDKN NATDGDLLIA
LALGRAGKRF QRPDYIQDAM AIYGDVLNLM TMKAGPYVVL MPGAVGFTKK DSVILNLSYY
VMPSLLQAFD LTADPRWRQV MEDGIRLVSA GRFGQWRLPP DWLAVNRATG ALSIASGWPP
RFSYDAIRVP LYFYWAHMLA PNVLADFTRF WNNFGANALP GWVDLTTGAR SPYNAPPGYL
AVAECTGLDS AGELPTLDHA PDYYSAALTL LVYIARAEET IK
