GUNB_FUSOX
ID GUNB_FUSOX Reviewed; 462 AA.
AC P46236;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Putative endoglucanase type B;
DE EC=3.2.1.4;
DE AltName: Full=Cellulase;
DE AltName: Full=Endo-1,4-beta-glucanase;
DE Flags: Precursor;
OS Fusarium oxysporum (Fusarium vascular wilt).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium oxysporum species complex.
OX NCBI_TaxID=5507;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7959045; DOI=10.1016/0378-1119(94)90878-8;
RA Sheppard P.O., Grant F.J., Oort P.J., Sprecher C.A., Foster D.C.,
RA Hagen F.S., Upshall A., McKnight G.L., O'Hara P.J.;
RT "The use of conserved cellulase family-specific sequences to clone
RT cellulase homologue cDNAs from Fusarium oxysporum.";
RL Gene 150:163-167(1994).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 6 (cellulase B) family.
CC {ECO:0000305}.
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DR EMBL; L29377; AAA65585.1; -; mRNA.
DR AlphaFoldDB; P46236; -.
DR SMR; P46236; -.
DR CAZy; CBM1; Carbohydrate-Binding Module Family 1.
DR CAZy; GH6; Glycoside Hydrolase Family 6.
DR VEuPathDB; FungiDB:FOC1_g10002687; -.
DR VEuPathDB; FungiDB:FOC4_g10009603; -.
DR VEuPathDB; FungiDB:FOIG_13447; -.
DR VEuPathDB; FungiDB:FOMG_14546; -.
DR VEuPathDB; FungiDB:FOXG_09643; -.
DR VEuPathDB; FungiDB:FOZG_13867; -.
DR VEuPathDB; FungiDB:HZS61_005819; -.
DR GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030248; F:cellulose binding; IEA:InterPro.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.40; -; 1.
DR InterPro; IPR016288; Beta_cellobiohydrolase.
DR InterPro; IPR036434; Beta_cellobiohydrolase_sf.
DR InterPro; IPR035971; CBD_sf.
DR InterPro; IPR000254; Cellulose-bd_dom_fun.
DR InterPro; IPR001524; Glyco_hydro_6_CS.
DR PANTHER; PTHR34876; PTHR34876; 1.
DR Pfam; PF00734; CBM_1; 1.
DR Pfam; PF01341; Glyco_hydro_6; 1.
DR PIRSF; PIRSF001100; Beta_cellobiohydrolase; 1.
DR PRINTS; PR00733; GLHYDRLASE6.
DR SMART; SM00236; fCBD; 1.
DR SUPFAM; SSF51989; SSF51989; 1.
DR SUPFAM; SSF57180; SSF57180; 1.
DR PROSITE; PS00562; CBM1_1; 1.
DR PROSITE; PS51164; CBM1_2; 1.
DR PROSITE; PS00655; GLYCOSYL_HYDROL_F6_1; 1.
DR PROSITE; PS00656; GLYCOSYL_HYDROL_F6_2; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cellulose degradation; Disulfide bond;
KW Glycoprotein; Glycosidase; Hydrolase; Polysaccharide degradation; Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..462
FT /note="Putative endoglucanase type B"
FT /id="PRO_0000007910"
FT DOMAIN 25..61
FT /note="CBM1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00597"
FT REGION 64..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 66..99
FT /note="Linker"
FT REGION 100..462
FT /note="Catalytic"
FT ACT_SITE 190
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10056"
FT ACT_SITE 236
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10057"
FT ACT_SITE 416
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10056"
FT CARBOHYD 37
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 223
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 272
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 317
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 33..50
FT /evidence="ECO:0000250"
FT DISULFID 44..60
FT /evidence="ECO:0000250"
FT DISULFID 191..250
FT /evidence="ECO:0000250"
FT DISULFID 383..430
FT /evidence="ECO:0000250"
SQ SEQUENCE 462 AA; 49208 MW; E25B2F5B828B637F CRC64;
MAYKLILAAF AATALAAPVE ERQSCSNGVW AQCGGQNWSG TPCCTSGNKC VKLNDFYSQC
QPGSAEPSST AAGPSSTTAT KTTATGGSST TAGGSVTSAP PAASDNPYAG VDLWANNYYR
SEVMNLAVPK LSGAKATAAA KVADVPSFQW MDTYDHISLM EDTLADIRKA NKAGGKYAGQ
FVVYDLPNRD CAAAASNGEY SLDKDGANKY KAYIAKIKGI LQNYSDTKVI LVIEPDSLAN
LVTNLNVDKC AKAESAYKEL TVYAIKELNL PNVSMYLDAG HGGWLGWPAN IGPAAKLYAQ
IYKDAGKPSR VRGLVTNVSN YNGWKLSTKP DYTESNPNYD EQRYINAFAP LLAQEGWSNV
KFIVDQGRSG KQPTGQKAQG DWCNAKGTGF GLRPSTNTGD ALADAFVWVK PGGESDGTSD
TSAARYDYHC GLDDALKPAP EAGTWFQAYF EQLLDNANPS FL