ID   GUNB_PAELA              Reviewed;         566 AA.
AC   P23550;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Endoglucanase B;
DE            EC=3.2.1.4;
DE   AltName: Full=Cellulase B;
DE   AltName: Full=Endo-1,4-beta-glucanase B;
DE            Short=EG-B;
DE   Flags: Precursor;
GN   Name=celB;
OS   Paenibacillus lautus (Bacillus lautus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=1401;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PL236;
RX   PubMed=2227426; DOI=10.1016/0378-1119(90)90135-e;
RA   Joergensen P.L., Hansen C.K.;
RT   "Multiple endo-beta-1,4-glucanase-encoding genes from Bacillus lautus PL236
RT   and characterization of the celB gene.";
RL   Gene 93:55-60(1990).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000305}.
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DR   EMBL; M33762; AAA22408.1; -; Genomic_DNA.
DR   PIR; JH0218; JH0218.
DR   AlphaFoldDB; P23550; -.
DR   SMR; P23550; -.
DR   CAZy; CBM46; Carbohydrate-Binding Module Family 46.
DR   CAZy; GH5; Glycoside Hydrolase Family 5.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR005102; Carbo-bd_X2.
DR   InterPro; IPR040946; CBM46.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR018087; Glyco_hydro_5_CS.
DR   InterPro; IPR016282; Glyco_hydro_5_endoGlcnase_B.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   Pfam; PF18448; CBM46; 1.
DR   Pfam; PF03442; CBM_X2; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   PIRSF; PIRSF001043; Endoglucanase_B; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cellulose degradation; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Signal.
FT   SIGNAL          1..30
FT   CHAIN           31..566
FT                   /note="Endoglucanase B"
FT                   /id="PRO_0000007835"
FT   ACT_SITE        177
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        299
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   566 AA;  62622 MW;  6426C8D8E29022C9 CRC64;
     MKKRRSSKVI LSLAIVVALL AAVEPNAALA AAPPSAMQSY VEAMQPGWNL GNSLDAVGAD
     ETLARGNPRI TKELIQNIAA QGYKSIRIPV TWDSHIGAAP NYQIEAAYLN RVQEVVQWAL
     DANLYVMINV HHDSWLWISK MESQHDQVLA RYNAIWTQIA NKFKNSPSKL MFESVNEPRF
     TDGGTTDEAK QQKMLDELNV SFFNIVRNSG GQNATRPLVL STLEASPTQE RMTALYNTMT
     KLNDKNLIAT VHFYGFWPFS VNIAGYTKFD AETQNDIITT FDNVYNTFVA KGIPVVVGEY
     GLLGFDKNTG VIEQGEKLKF FEFFAQYVKQ KSISTMLWDN GQHFNRTSFK WSDPDLFNMI
     KASWTGRSST ASSDLIHVKQ GTAVKDTSVQ LNLNGNTLTS LSVNGTTLKS GTDYTLNSSR
     LTFKASQLTK LTSLGKLGVN ATIVTKFNRG ADWKFNVVLY NTPKLSSTTG TTSSFAIPTA
     FNGDQLATME AVYVNGGNAG PHNWTSFKEF ETTFSPAYSE GKIKLQQAFF NEVNDTTVTL
     KFQFWSGEIV NYTIKKSGST VTGTAS