GUNC_FUSOX
ID GUNC_FUSOX Reviewed; 429 AA.
AC P46237;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Endoglucanase type C;
DE EC=3.2.1.4;
DE AltName: Full=Cellulase;
DE AltName: Full=Endo-1,4-beta-glucanase;
DE AltName: Full=Endoglucanase I;
DE Short=EG I;
DE Flags: Precursor;
OS Fusarium oxysporum (Fusarium vascular wilt).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium oxysporum species complex.
OX NCBI_TaxID=5507;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7959045; DOI=10.1016/0378-1119(94)90878-8;
RA Sheppard P.O., Grant F.J., Oort P.J., Sprecher C.A., Foster D.C.,
RA Hagen F.S., Upshall A., McKnight G.L., O'Hara P.J.;
RT "The use of conserved cellulase family-specific sequences to clone
RT cellulase homologue cDNAs from Fusarium oxysporum.";
RL Gene 150:163-167(1994).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), AND PYROGLUTAMATE FORMATION AT
RP GLN-19.
RX PubMed=8952478; DOI=10.1021/bi961946h;
RA Sulzenbacher G., Driguez H., Henrissat B., Schuelein M., Davies G.J.;
RT "Structure of the Fusarium oxysporum endoglucanase I with a nonhydrolyzable
RT substrate analogue: substrate distortion gives rise to the preferred axial
RT orientation for the leaving group.";
RL Biochemistry 35:15280-15287(1996).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RX PubMed=9153432; DOI=10.1021/bi962963+;
RA Sulzenbacher G., Schuelein M., Davies G.J.;
RT "Structure of the endoglucanase I from Fusarium oxysporum: native,
RT cellobiose, and 3,4-epoxybutyl beta-D-cellobioside-inhibited forms, at 2.3-
RT A resolution.";
RL Biochemistry 36:5902-5911(1997).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 7 (cellulase C) family.
CC {ECO:0000305}.
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DR EMBL; L29378; AAA65586.1; -; mRNA.
DR PDB; 1OVW; X-ray; 2.70 A; A/B/C/D=20-416.
DR PDB; 2OVW; X-ray; 2.30 A; A/B/C/D=20-429.
DR PDB; 3OVW; X-ray; 2.30 A; A/B=20-429.
DR PDB; 4OVW; X-ray; 2.30 A; A/B=20-429.
DR PDBsum; 1OVW; -.
DR PDBsum; 2OVW; -.
DR PDBsum; 3OVW; -.
DR PDBsum; 4OVW; -.
DR AlphaFoldDB; P46237; -.
DR SMR; P46237; -.
DR CAZy; GH7; Glycoside Hydrolase Family 7.
DR CLAE; EGL7A_FUSOX; -.
DR VEuPathDB; FungiDB:FOC1_g10015774; -.
DR VEuPathDB; FungiDB:FOC4_g10015049; -.
DR VEuPathDB; FungiDB:FOIG_01874; -.
DR VEuPathDB; FungiDB:FOMG_10993; -.
DR VEuPathDB; FungiDB:FOXG_02912; -.
DR VEuPathDB; FungiDB:FOZG_09209; -.
DR VEuPathDB; FungiDB:HZS61_015031; -.
DR EvolutionaryTrace; P46237; -.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR CDD; cd07999; GH7_CBH_EG; 1.
DR Gene3D; 2.70.100.10; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001722; Glyco_hydro_7.
DR InterPro; IPR037019; Glyco_hydro_7_sf.
DR PANTHER; PTHR33753; PTHR33753; 1.
DR Pfam; PF00840; Glyco_hydro_7; 1.
DR PRINTS; PR00734; GLHYDRLASE7.
DR SUPFAM; SSF49899; SSF49899; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Cellulose degradation;
KW Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Pyrrolidone carboxylic acid; Signal.
FT SIGNAL 1..18
FT CHAIN 19..429
FT /note="Endoglucanase type C"
FT /id="PRO_0000007912"
FT ACT_SITE 215
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 220
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT MOD_RES 19
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:8952478"
FT CARBOHYD 74
FT /note="N-linked (GlcNAc...) asparagine"
FT CARBOHYD 265
FT /note="N-linked (GlcNAc...) asparagine"
FT CARBOHYD 318
FT /note="N-linked (GlcNAc...) asparagine"
FT DISULFID 36..42
FT DISULFID 66..88
FT DISULFID 78..84
FT DISULFID 156..383
FT DISULFID 190..213
FT DISULFID 194..212
FT DISULFID 233..252
FT DISULFID 241..246
FT DISULFID 257..333
FT STRAND 30..37
FT /evidence="ECO:0007829|PDB:2OVW"
FT TURN 38..40
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 41..51
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 53..56
FT /evidence="ECO:0007829|PDB:2OVW"
FT TURN 75..77
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 81..87
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 95..100
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 103..106
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 109..116
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 124..128
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 132..135
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 144..150
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 158..165
FT /evidence="ECO:0007829|PDB:2OVW"
FT TURN 169..172
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 173..175
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 183..185
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 198..200
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 210..212
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 215..221
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 226..231
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 233..235
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 239..241
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 243..246
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 250..252
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 261..264
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 269..273
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 276..279
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 284..292
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 298..307
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 310..313
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 319..322
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 326..328
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 330..335
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 339..343
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 346..356
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 358..366
FT /evidence="ECO:0007829|PDB:2OVW"
FT TURN 368..372
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 373..376
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 378..380
FT /evidence="ECO:0007829|PDB:2OVW"
FT HELIX 390..396
FT /evidence="ECO:0007829|PDB:2OVW"
FT STRAND 401..411
FT /evidence="ECO:0007829|PDB:2OVW"
SQ SEQUENCE 429 AA; 46445 MW; FAE8A880B1F880F9 CRC64;
MKSLSLILSA LAVQVAVAQT PDKAKEQHPK LETYRCTKAS GCKKQTNYIV ADAGIHGIRQ
KNGAGCGDWG QKPNATACPD EASCAKNCIL SGMDSNAYKN AGITTSGNKL RLQQLINNQL
VSPRVYLLEE NKKKYEMLHL TGTEFSFDVE MEKLPCGMNG ALYLSEMPQD GGKSTSRNSK
AGAYYGAGYC DAQCYVTPFI NGVGNIKGQG VCCNELDIWE ANSRATHIAP HPCSKPGLYG
CTGDECGSSG ICDKAGCGWN HNRINVTDFY GRGKQYKVDS TRKFTVTSQF VANKQGDLIE
LHRHYIQDNK VIESAVVNIS GPPKINFIND KYCAATGANE YMRLGGTKQM GDAMSRGMVL
AMSVWWSEGD FMAWLDQGVA GPCDATEGDP KNIVKVQPNP EVTFSNIRIG EIGSTSSVKA
PAYPGPHRL
