GUND_CLOC7
ID GUND_CLOC7 Reviewed; 515 AA.
AC P28623; D9SUH3;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=Endoglucanase D;
DE EC=3.2.1.4;
DE AltName: Full=Cellulase D;
DE AltName: Full=Endo-1,4-beta-glucanase D;
DE Flags: Precursor;
GN Name=engD; OrderedLocusNames=Clocel_3242;
OS Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=573061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 32-44.
RX PubMed=1538700; DOI=10.1007/bf00292718;
RA Hamamoto T., Foong F., Shoseyov O., Doi R.H.;
RT "Analysis of functional domains of endoglucanases from Clostridium
RT cellulovorans by gene cloning, nucleotide sequencing and chimeric protein
RT construction.";
RL Mol. Gen. Genet. 231:472-479(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35296 / DSM 3052 / OCM 3 / 743B;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA Chang Y.-J., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N.,
RA Hemme C.L., Woyke T.;
RT "Complete sequence of Clostridium cellulovorans 743B.";
RL Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Has endoglucanase activity on carboxymethyl-cellulose (CMC),
CC cellobiosidase activity on p-nitrophenyl-cellobioside (p-NPC), and
CC partial hydrolytic activity on crystalline cellulose (Avicel).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000305}.
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DR EMBL; M37434; AAA23233.1; -; Genomic_DNA.
DR EMBL; CP002160; ADL52928.1; -; Genomic_DNA.
DR PIR; S20493; S20493.
DR RefSeq; WP_010073314.1; NC_014393.1.
DR PDB; 3NDY; X-ray; 2.10 A; A/B/C/D=32-376, E/F/G/H=409-515.
DR PDB; 3NDZ; X-ray; 2.08 A; A/B/C/D=32-376, E/F/G/H=409-515.
DR PDBsum; 3NDY; -.
DR PDBsum; 3NDZ; -.
DR AlphaFoldDB; P28623; -.
DR SMR; P28623; -.
DR STRING; 573061.Clocel_3242; -.
DR CAZy; CBM2; Carbohydrate-Binding Module Family 2.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR EnsemblBacteria; ADL52928; ADL52928; Clocel_3242.
DR KEGG; ccb:Clocel_3242; -.
DR eggNOG; COG2730; Bacteria.
DR eggNOG; COG5297; Bacteria.
DR HOGENOM; CLU_018668_2_1_9; -.
DR OMA; YVILNTH; -.
DR OrthoDB; 1395441at2; -.
DR EvolutionaryTrace; P28623; -.
DR Proteomes; UP000002730; Chromosome.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030247; F:polysaccharide binding; IEA:InterPro.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.290; -; 1.
DR InterPro; IPR001919; CBD2.
DR InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR InterPro; IPR012291; CBM2_carb-bd_dom_sf.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR018087; Glyco_hydro_5_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF00553; CBM_2; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SMART; SM00637; CBD_II; 1.
DR SUPFAM; SSF49384; SSF49384; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS51173; CBM2; 1.
DR PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Cellulose degradation;
KW Direct protein sequencing; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000269|PubMed:1538700"
FT CHAIN 32..515
FT /note="Endoglucanase D"
FT /id="PRO_0000007848"
FT DOMAIN 404..515
FT /note="CBM2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01135"
FT REGION 32..376
FT /note="Catalytic"
FT /evidence="ECO:0000250"
FT REGION 372..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 385..402
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 180
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 303
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT CONFLICT 188..189
FT /note="NE -> LQ (in Ref. 1; AAA23233)"
FT /evidence="ECO:0000305"
FT HELIX 43..50
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 52..56
FT /evidence="ECO:0007829|PDB:3NDZ"
FT TURN 57..61
FT /evidence="ECO:0007829|PDB:3NDY"
FT TURN 66..69
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 76..85
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 89..92
FT /evidence="ECO:0007829|PDB:3NDZ"
FT TURN 103..105
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 110..124
FT /evidence="ECO:0007829|PDB:3NDZ"
FT TURN 125..127
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 129..132
FT /evidence="ECO:0007829|PDB:3NDZ"
FT TURN 138..140
FT /evidence="ECO:0007829|PDB:3NDZ"
FT TURN 145..147
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 148..165
FT /evidence="ECO:0007829|PDB:3NDZ"
FT TURN 166..168
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 173..177
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 186..188
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 195..214
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 217..220
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 224..227
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 229..231
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 235..240
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 244..246
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 250..255
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 260..263
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 275..291
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 293..295
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 299..304
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 312..327
FT /evidence="ECO:0007829|PDB:3NDZ"
FT TURN 328..330
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 332..337
FT /evidence="ECO:0007829|PDB:3NDZ"
FT TURN 354..357
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 358..361
FT /evidence="ECO:0007829|PDB:3NDZ"
FT HELIX 362..372
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 411..420
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 422..433
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 435..437
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 439..441
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 443..447
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 452..465
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 468..473
FT /evidence="ECO:0007829|PDB:3NDZ"
FT TURN 482..484
FT /evidence="ECO:0007829|PDB:3NDZ"
FT STRAND 485..495
FT /evidence="ECO:0007829|PDB:3NDZ"
SQ SEQUENCE 515 AA; 55978 MW; 57F15B035856008E CRC64;
MIKHLLSRGK LLLFVSVMAT SSIIAGGNAY GSTAFTGVRD VPAQQIVNEM KVGWNLGNTM
DAIGGETNWG NPMTTHAMIN KIKEAGFNTL RLPVTWDGHM GAAPEYTIDQ TWMKRVEEIA
NYAFDNDMYV IINLHHENEW LKPFYANEAQ VKAQLTKVWT QIANNFKKYG DHLIFETMNE
PRPVGASNEW TGGSYENREV VNRYNLTAVN AIRATGGNNA TRYIMVPTLA ASAMSTTIND
LVIPNNDSKV IVSLHMYSPY FFAMDINGTS SWGSDYDKSS LDSEFDAVYN KFVKNGRAVV
IGEMGSINKN NTAARVTHAE YYAKSAKARG LTPIWWDNGY SVAGKAETFG IFNRSNLTWD
APEVMKAFIK GIGGSSTTTP TTPTTPTTPT TPTTPTTPTT PTTPTTPQSA VEVTYAITNS
WGSGASVNVT IKNNGTTPIN GWTLKWTMPI NQTITNMWSA SFVASGTTLS VTNAGYNGTI
AANGGTQSFG FNINYSGVLS KPTGFTVNGT ECTVK
