ID   GUNE_RUMFL              Reviewed;         320 AA.
AC   Q05622;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Endoglucanase E;
DE            Short=EGE;
DE            EC=3.2.1.4;
DE   AltName: Full=Cellulase;
DE   AltName: Full=Endo-1,4-beta-glucanase E;
DE   Flags: Precursor;
GN   Name=celE;
OS   Ruminococcus flavefaciens.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Ruminococcus.
OX   NCBI_TaxID=1265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=FD-1;
RX   PubMed=8360615; DOI=10.1099/00221287-139-6-1219;
RA   Wang W., Reid S.J., Thomson J.A.;
RT   "Transcriptional regulation of an endoglucanase and a cellodextrinase gene
RT   in Ruminococcus flavefaciens FD-1.";
RL   J. Gen. Microbiol. 139:1219-1226(1993).
CC   -!- FUNCTION: Hydrolyzes both carboxymethylcellulose and xylan. Probably
CC       has a role in hydrolyzing oligosaccharides derived from cellulose,
CC       which are transported across the cell wall.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted.
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DR   EMBL; L03800; AAA26468.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q05622; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0045493; P:xylan catabolic process; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cellulose degradation; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Secreted; Signal; Xylan degradation.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..320
FT                   /note="Endoglucanase E"
FT                   /id="PRO_0000012253"
FT   ACT_SITE        150
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   320 AA;  35938 MW;  DD5C549423405AFD CRC64;
     MRKRITSMIT VISLTLCGTI GTYPHNANAS AKEAFEDISK RSECTKVFDN DNGTYTAYSN
     TAPIHYLNND EWKEIDNTLI EDSDDYYRNK DNSFNIYIPK EYSLGKNIKN PVIMNYDTFS
     LSTSITDIKM PDRPEEETFS EGVYADINNE VYTTIDNSAI AEGMKAALKK SASMATYHSI
     VEDIDLDIAV HNASVSESII INKLESLPET ITYSISVEDA IIKKTEDNRL QLIKDNEAVL
     ILSPFTISDS SENVNVMQVE YDLTESEEGY EVTLYPAETV NRMSSPVMPL SLGSEYSYDR
     PFSTVYNSQA SLHPSIIIIT