GUNH_ACET2
ID GUNH_ACET2 Reviewed; 900 AA.
AC P16218; A3DFH2;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Endoglucanase H;
DE EC=3.2.1.4;
DE AltName: Full=Cellulase H;
DE AltName: Full=Endo-1,4-beta-glucanase H;
DE Short=EgH;
DE Flags: Precursor;
GN Name=celH; OrderedLocusNames=Cthe_1472;
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2197182; DOI=10.1016/0378-1119(90)90206-7;
RA Yaguee E., Beguin P., Aubert J.-P.;
RT "Nucleotide sequence and deletion analysis of the cellulase-encoding gene
RT celH of Clostridium thermocellum.";
RL Gene 89:61-67(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: This enzyme catalyzes the endohydrolysis of 1,4-beta-
CC glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SIMILARITY: In the N-terminal section; belongs to the glycosyl
CC hydrolase 5 (cellulase A) family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the glycosyl
CC hydrolase 26 family. {ECO:0000305}.
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DR EMBL; M31903; AAA23225.1; -; Genomic_DNA.
DR EMBL; CP000568; ABN52701.1; -; Genomic_DNA.
DR PIR; JH0157; JH0157.
DR RefSeq; WP_011838089.1; NC_009012.1.
DR PDB; 1V0A; X-ray; 1.98 A; A=655-821.
DR PDB; 2BV9; X-ray; 1.50 A; A=26-304.
DR PDB; 2BVD; X-ray; 1.60 A; A=26-304.
DR PDB; 2CIP; X-ray; 1.40 A; A=26-304.
DR PDB; 2CIT; X-ray; 1.40 A; A=26-304.
DR PDB; 2LRO; NMR; -; A=655-821.
DR PDB; 2LRP; NMR; -; A=655-821.
DR PDB; 2V3G; X-ray; 1.20 A; A=26-305.
DR PDB; 2VI0; X-ray; 1.51 A; A=26-304.
DR PDB; 4U3A; X-ray; 2.42 A; A/B=290-654.
DR PDB; 4U5I; X-ray; 2.50 A; A/B=290-654.
DR PDB; 4U5K; X-ray; 2.65 A; A/B=290-654.
DR PDB; 5BYW; X-ray; 2.60 A; A/B/C/D/E=290-654.
DR PDB; 6R31; X-ray; 2.60 A; A=655-821.
DR PDB; 6R3M; X-ray; 1.45 A; A=655-821.
DR PDB; 6ZPL; X-ray; 3.94 A; C=31-303.
DR PDBsum; 1V0A; -.
DR PDBsum; 2BV9; -.
DR PDBsum; 2BVD; -.
DR PDBsum; 2CIP; -.
DR PDBsum; 2CIT; -.
DR PDBsum; 2LRO; -.
DR PDBsum; 2LRP; -.
DR PDBsum; 2V3G; -.
DR PDBsum; 2VI0; -.
DR PDBsum; 4U3A; -.
DR PDBsum; 4U5I; -.
DR PDBsum; 4U5K; -.
DR PDBsum; 5BYW; -.
DR PDBsum; 6R31; -.
DR PDBsum; 6R3M; -.
DR PDBsum; 6ZPL; -.
DR AlphaFoldDB; P16218; -.
DR BMRB; P16218; -.
DR SMR; P16218; -.
DR STRING; 203119.Cthe_1472; -.
DR DrugBank; DB08785; 4-Methylcoumarin.
DR CAZy; CBM11; Carbohydrate-Binding Module Family 11.
DR CAZy; GH26; Glycoside Hydrolase Family 26.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR PRIDE; P16218; -.
DR EnsemblBacteria; ABN52701; ABN52701; Cthe_1472.
DR KEGG; cth:Cthe_1472; -.
DR eggNOG; COG2730; Bacteria.
DR eggNOG; COG4124; Bacteria.
DR HOGENOM; CLU_321778_0_0_9; -.
DR OMA; WRINSSP; -.
DR OrthoDB; 1395441at2; -.
DR BioCyc; MetaCyc:MON-16422; -.
DR EvolutionaryTrace; P16218; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0008810; F:cellulase activity; IMP:MENGO.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1330.10; -; 1.
DR InterPro; IPR005087; CBM_fam11.
DR InterPro; IPR002105; Dockerin_1_rpt.
DR InterPro; IPR016134; Dockerin_dom.
DR InterPro; IPR036439; Dockerin_dom_sf.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR022790; GH26_dom.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR018087; Glyco_hydro_5_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF03425; CBM_11; 1.
DR Pfam; PF00150; Cellulase; 1.
DR Pfam; PF00404; Dockerin_1; 1.
DR Pfam; PF02156; Glyco_hydro_26; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51445; SSF51445; 2.
DR SUPFAM; SSF63446; SSF63446; 1.
DR PROSITE; PS00448; CLOS_CELLULOSOME_RPT; 2.
DR PROSITE; PS51766; DOCKERIN; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS51764; GH26; 1.
DR PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Cellulose degradation; Glycosidase;
KW Hydrolase; Polysaccharide degradation; Reference proteome; Signal.
FT SIGNAL 1..44
FT CHAIN 45..900
FT /note="Endoglucanase H"
FT /id="PRO_0000007854"
FT DOMAIN 45..298
FT /note="GH26"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01100"
FT DOMAIN 655..900
FT /note="CBM11"
FT DOMAIN 827..900
FT /note="Dockerin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01102"
FT REGION 300..630
FT /note="Catalytic"
FT /evidence="ECO:0000250"
FT REGION 303..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 131
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01100"
FT ACT_SITE 244
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01100"
FT ACT_SITE 460
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 565
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT STRAND 32..36
FT /evidence="ECO:0007829|PDB:2V3G"
FT HELIX 43..53
FT /evidence="ECO:0007829|PDB:2V3G"
FT STRAND 58..65
FT /evidence="ECO:0007829|PDB:2V3G"
FT HELIX 70..82
FT /evidence="ECO:0007829|PDB:2V3G"
FT STRAND 86..92
FT /evidence="ECO:0007829|PDB:2V3G"
FT HELIX 98..102
FT /evidence="ECO:0007829|PDB:2V3G"
FT TURN 103..106
FT /evidence="ECO:0007829|PDB:2V3G"
FT HELIX 107..120
FT /evidence="ECO:0007829|PDB:2V3G"
FT STRAND 124..129
FT /evidence="ECO:0007829|PDB:2V3G"
FT STRAND 134..137
FT /evidence="ECO:0007829|PDB:2V3G"
FT HELIX 150..166
FT /evidence="ECO:0007829|PDB:2V3G"
FT STRAND 172..175
FT /evidence="ECO:0007829|PDB:2V3G"
FT STRAND 178..181
FT /evidence="ECO:0007829|PDB:2V3G"
FT HELIX 196..198
FT /evidence="ECO:0007829|PDB:2V3G"
FT STRAND 200..208
FT /evidence="ECO:0007829|PDB:2V3G"
FT TURN 214..216
FT /evidence="ECO:0007829|PDB:2V3G"
FT HELIX 222..233
FT /evidence="ECO:0007829|PDB:2V3G"
FT STRAND 236..238
FT /evidence="ECO:0007829|PDB:2V3G"
FT STRAND 240..247
FT /evidence="ECO:0007829|PDB:2V3G"
FT HELIX 254..268
FT /evidence="ECO:0007829|PDB:2V3G"
FT STRAND 272..278
FT /evidence="ECO:0007829|PDB:2V3G"
FT STRAND 281..285
FT /evidence="ECO:0007829|PDB:2V3G"
FT HELIX 292..301
FT /evidence="ECO:0007829|PDB:2V3G"
FT HELIX 329..336
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 338..341
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 348..350
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 353..355
FT /evidence="ECO:0007829|PDB:4U3A"
FT HELIX 360..369
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 373..376
FT /evidence="ECO:0007829|PDB:4U3A"
FT HELIX 381..383
FT /evidence="ECO:0007829|PDB:4U3A"
FT HELIX 394..409
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 413..417
FT /evidence="ECO:0007829|PDB:4U3A"
FT HELIX 423..426
FT /evidence="ECO:0007829|PDB:4U3A"
FT HELIX 428..445
FT /evidence="ECO:0007829|PDB:4U3A"
FT TURN 446..448
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 453..456
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 463..465
FT /evidence="ECO:0007829|PDB:4U3A"
FT HELIX 467..484
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 490..497
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 502..504
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 512..520
FT /evidence="ECO:0007829|PDB:4U3A"
FT HELIX 524..527
FT /evidence="ECO:0007829|PDB:5BYW"
FT HELIX 537..557
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 561..566
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 571..573
FT /evidence="ECO:0007829|PDB:4U3A"
FT HELIX 574..590
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 594..597
FT /evidence="ECO:0007829|PDB:4U3A"
FT HELIX 606..608
FT /evidence="ECO:0007829|PDB:5BYW"
FT STRAND 612..614
FT /evidence="ECO:0007829|PDB:4U3A"
FT TURN 615..618
FT /evidence="ECO:0007829|PDB:4U3A"
FT HELIX 622..628
FT /evidence="ECO:0007829|PDB:4U3A"
FT STRAND 656..662
FT /evidence="ECO:0007829|PDB:6R3M"
FT STRAND 664..666
FT /evidence="ECO:0007829|PDB:6R3M"
FT STRAND 671..675
FT /evidence="ECO:0007829|PDB:6R3M"
FT STRAND 679..686
FT /evidence="ECO:0007829|PDB:6R3M"
FT STRAND 688..698
FT /evidence="ECO:0007829|PDB:6R3M"
FT STRAND 704..710
FT /evidence="ECO:0007829|PDB:6R3M"
FT STRAND 721..728
FT /evidence="ECO:0007829|PDB:6R3M"
FT STRAND 730..732
FT /evidence="ECO:0007829|PDB:2LRP"
FT STRAND 736..743
FT /evidence="ECO:0007829|PDB:6R3M"
FT STRAND 745..758
FT /evidence="ECO:0007829|PDB:6R3M"
FT STRAND 765..770
FT /evidence="ECO:0007829|PDB:6R3M"
FT HELIX 771..773
FT /evidence="ECO:0007829|PDB:6R3M"
FT STRAND 788..790
FT /evidence="ECO:0007829|PDB:2LRP"
FT STRAND 795..806
FT /evidence="ECO:0007829|PDB:6R3M"
FT STRAND 808..819
FT /evidence="ECO:0007829|PDB:6R3M"
SQ SEQUENCE 900 AA; 102416 MW; 973AFB1954FC246B CRC64;
MKKRLLVSFL VLSIIVGLLS FQSLGNYNSG LKIGAWVGTQ PSESAIKSFQ ELQGRKLDIV
HQFINWSTDF SWVRPYADAV YNNGSILMIT WEPWEYNTVD IKNGKADAYI TRMAQDMKAY
GKEIWLRPLH EANGDWYPWA IGYSSRVNTN ETYIAAFRHI VDIFRANGAT NVKWVFNVNC
DNVGNGTSYL GHYPGDNYVD YTSIDGYNWG TTQSWGSQWQ SFDQVFSRAY QALASINKPI
IIAEFASAEI GGNKARWITE AYNSIRTSYN KVIAAVWFHE NKETDWRINS SPEALAAYRE
AIGAGSSNPT PTPTWTSTPP SSSPKAVDPF EMVRKMGMGT NLGNTLEAPY EGSWSKSAME
YYFDDFKAAG YKNVRIPVRW DNHTMRTYPY TIDKAFLDRV EQVVDWSLSR GFVTIINSHH
DDWIKEDYNG NIERFEKIWE QIAERFKNKS ENLLFEIMNE PFGNITDEQI DDMNSRILKI
IRKTNPTRIV IIGGGYWNSY NTLVNIKIPD DPYLIGTFHY YDPYEFTHKW RGTWGTQEDM
DTVVRVFDFV KSWSDRNNIP VYFGEFAVMA YADRTSRVKW YDFISDAALE RGFACSVWDN
GVFGSLDNDM AIYNRDTRTF DTEILNALFN PGTYPSYSPK PSPTPRPTKP PVTPAVGEKM
LDDFEGVLNW GSYSGEGAKV STKIVSGKTG NGMEVSYTGT TDGYWGTVYS LPDGDWSKWL
KISFDIKSVD GSANEIRFMI AEKSINGVGD GEHWVYSITP DSSWKTIEIP FSSFRRRLDY
QPPGQDMSGT LDLDNIDSIH FMYANNKSGK FVVDNIKLIG ATSDPTPSIK HGDLNFDNAV
NSTDLLMLKR YILKSLELGT SEQEEKFKKA ADLNRDNKVD STDLTILKRY LLKAISEIPI
