ID   GUNS_PECPM              Reviewed;         264 AA.
AC   P16630; K4FJ91;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Endoglucanase S;
DE            EC=3.2.1.4;
DE   AltName: Full=Cellulase S;
DE   AltName: Full=Endo-1,4-beta-glucanase S;
DE   Flags: Precursor;
GN   Name=celS; OrderedLocusNames=W5S_2949;
OS   Pectobacterium parmentieri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=1905730;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=SCC3193;
RX   PubMed=2379837; DOI=10.1016/0378-1119(90)90433-r;
RA   Saarilahti H.T., Henrissat B., Palva E.T.;
RT   "CelS: a novel endoglucanase identified from Erwinia carotovora subsp.
RT   carotovora.";
RL   Gene 90:9-14(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SCC3193;
RX   PubMed=23045508; DOI=10.1128/jb.00681-12;
RA   Koskinen J.P., Laine P., Niemi O., Nykyri J., Harjunpaa H., Auvinen P.,
RA   Paulin L., Pirhonen M., Palva T., Holm L.;
RT   "Genome sequence of Pectobacterium sp. strain SCC3193.";
RL   J. Bacteriol. 194:6004-6004(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 12 (cellulase H) family.
CC       {ECO:0000305}.
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DR   EMBL; M32399; AAA24817.1; -; Genomic_DNA.
DR   EMBL; CP003415; AFI91032.1; -; Genomic_DNA.
DR   PIR; JU0328; JU0328.
DR   RefSeq; WP_014700567.1; NC_017845.1.
DR   AlphaFoldDB; P16630; -.
DR   SMR; P16630; -.
DR   STRING; 1905730.W5S_2949; -.
DR   CAZy; GH12; Glycoside Hydrolase Family 12.
DR   EnsemblBacteria; AFI91032; AFI91032; W5S_2949.
DR   KEGG; pec:W5S_2949; -.
DR   PATRIC; fig|1166016.3.peg.2995; -.
DR   eggNOG; COG5297; Bacteria.
DR   HOGENOM; CLU_051064_1_2_6; -.
DR   OrthoDB; 797310at2; -.
DR   Proteomes; UP000008044; Chromosome.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.180; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR013319; GH11/12.
DR   InterPro; IPR002594; GH12.
DR   PANTHER; PTHR34002; PTHR34002; 1.
DR   Pfam; PF01670; Glyco_hydro_12; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Cellulose degradation; Direct protein sequencing;
KW   Glycosidase; Hydrolase; Polysaccharide degradation; Signal.
FT   SIGNAL          1..32
FT   CHAIN           33..264
FT                   /note="Endoglucanase S"
FT                   /id="PRO_0000008020"
SQ   SEQUENCE   264 AA;  29757 MW;  E6D61388950C77AA CRC64;
     MQTVNTQPHR IFRVLLPAVF SSLLLSSLTV SAASSSNDAD KLYFGNNKYY LFNNVWGKDE
     IKGWQQTIFY NSPISMGWNW HWPSSTHSVK AYPSLVSGWH WTAGYTENSG LPIQLSSNKS
     ITSNVTYSIK ATGTYNAAYD IWFHTTDKAN WDSSPTDELM IWLNDTNAGP AGDYIETVFL
     GDSSWNVFKG WINADNGGGW NVFSFVHTSG TNSASLNIRH FTDYLVQTKQ WMSDEKYISS
     VEFGTEIFGG DGQIDITEWR VDVK