ID   GUNY_DICD3              Reviewed;         332 AA.
AC   P27032; E0SES8;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Minor endoglucanase Y;
DE            EC=3.2.1.4;
DE   AltName: Full=Cellulase Y;
DE   AltName: Full=Endo-1,4-beta-glucanase Y;
DE            Short=EGY;
DE   Flags: Precursor;
GN   Name=celY; OrderedLocusNames=Dda3937_01994;
OS   Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Dickeya.
OX   NCBI_TaxID=198628;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 24-33.
RC   STRAIN=3937;
RX   PubMed=1937031; DOI=10.1016/0378-1119(91)90573-t;
RA   Guiseppi A., Aymeric J.-L., Cami B., Barras F., Creuzet N.;
RT   "Sequence analysis of the cellulase-encoding celY gene of Erwinia
RT   chrysanthemi: a possible case of interspecies gene transfer.";
RL   Gene 106:109-114(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=3937;
RX   PubMed=21217001; DOI=10.1128/jb.01513-10;
RA   Glasner J.D., Yang C.H., Reverchon S., Hugouvieux-Cotte-Pattat N.,
RA   Condemine G., Bohin J.P., Van Gijsegem F., Yang S., Franza T., Expert D.,
RA   Plunkett G. III, San Francisco M.J., Charkowski A.O., Py B., Bell K.,
RA   Rauscher L., Rodriguez-Palenzuela P., Toussaint A., Holeva M.C., He S.Y.,
RA   Douet V., Boccara M., Blanco C., Toth I., Anderson B.D., Biehl B.S.,
RA   Mau B., Flynn S.M., Barras F., Lindeberg M., Birch P.R., Tsuyumu S.,
RA   Shi X., Hibbing M., Yap M.N., Carpentier M., Dassa E., Umehara M.,
RA   Kim J.F., Rusch M., Soni P., Mayhew G.F., Fouts D.E., Gill S.R.,
RA   Blattner F.R., Keen N.T., Perna N.T.;
RT   "Genome sequence of the plant-pathogenic bacterium Dickeya dadantii 3937.";
RL   J. Bacteriol. 193:2076-2077(2011).
CC   -!- FUNCTION: Represents only 3% of the global cellulase activity.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 8 (cellulase D) family.
CC       {ECO:0000305}.
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DR   EMBL; M74044; AAA24818.1; -; Genomic_DNA.
DR   EMBL; CP002038; ADM96331.1; -; Genomic_DNA.
DR   PIR; JT0585; JT0585.
DR   RefSeq; WP_013315820.1; NC_014500.1.
DR   AlphaFoldDB; P27032; -.
DR   SMR; P27032; -.
DR   STRING; 198628.Dda3937_01994; -.
DR   CAZy; GH8; Glycoside Hydrolase Family 8.
DR   EnsemblBacteria; ADM96331; ADM96331; Dda3937_01994.
DR   GeneID; 9731500; -.
DR   KEGG; ddd:Dda3937_01994; -.
DR   PATRIC; fig|198628.6.peg.121; -.
DR   eggNOG; COG3405; Bacteria.
DR   HOGENOM; CLU_037297_1_0_6; -.
DR   OMA; EGQGFAM; -.
DR   OrthoDB; 636673at2; -.
DR   BioCyc; DDAD198628:DDA3937_RS00550-MON; -.
DR   Proteomes; UP000006859; Chromosome.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR002037; Glyco_hydro_8.
DR   InterPro; IPR019834; Glyco_hydro_8_CS.
DR   Pfam; PF01270; Glyco_hydro_8; 1.
DR   PRINTS; PR00735; GLHYDRLASE8.
DR   SUPFAM; SSF48208; SSF48208; 1.
DR   PROSITE; PS00812; GLYCOSYL_HYDROL_F8; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Cellulose degradation; Direct protein sequencing;
KW   Glycosidase; Hydrolase; Polysaccharide degradation; Reference proteome;
KW   Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000269|PubMed:1937031"
FT   CHAIN           24..332
FT                   /note="Minor endoglucanase Y"
FT                   /id="PRO_0000007937"
FT   ACT_SITE        53
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        110
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10058"
SQ   SEQUENCE   332 AA;  37593 MW;  6EA760A2B8227079 CRC64;
     MGKPMWRCWA LMLMVWFSAS ATAANGWEIY KSRFMTTDGR IQDTGNKNVS HTEGQGFAML
     MAVHYDDRIA FDNLWNWTQS HLRNTTSGLF YWRYDPSAAN PVVDKNNASD GDVLIAWALL
     KAGNKWQDNR YLQASDSIQK AIIASNIIQF AGRTVMLPGA YGFNKNSYVI LNPSYFLFPA
     WRDFANRSHL QVWRQLIDDS LSLVGEMRFG QVGLPTDWAA LNADGSMAPA TAWPSRFSYD
     AIRIPLYLYW YDAKTTALVP FQLYWRNYPR LTTPAWVDVL SSNTATYNMQ GGLLAVRDLT
     MGNLDGLSDL PGASEDYYSS SLRLLVMLAR GK