ID   GUN_MAREN               Reviewed;         349 AA.
AC   A0A023VXA2;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   09-JUL-2014, sequence version 1.
DT   03-AUG-2022, entry version 23.
DE   RecName: Full=Endoglucanase {ECO:0000303|PubMed:33523417};
DE            EC=3.2.1.4 {ECO:0000269|PubMed:33523417};
DE   Flags: Precursor;
GN   Name=cel5A {ECO:0000303|PubMed:33523417};
GN   ORFNames=TM49_09170 {ECO:0000312|EMBL:AJY45818.1};
OS   Martelella endophytica.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Aurantimonadaceae; Martelella.
OX   NCBI_TaxID=1486262;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ACTIVITY
RP   REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=NBRC 109149 / KCCM 43011 / YC6887;
RX   PubMed=33523417; DOI=10.1007/s12033-020-00295-3;
RA   Khan A., Khan H., Faheem M., Zeb A., Badshah M., Chung Y.R.;
RT   "Isolation and characterization of an acidic, salt-tolerant endoglucanase
RT   Cel5A from a bacterial strain Martelella endophytica YC6887 genome.";
RL   Mol. Biotechnol. 63:305-315(2021).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 109149 / KCCM 43011 / YC6887;
RX   PubMed=25953177; DOI=10.1128/genomea.00366-15;
RA   Khan A., Khan H., Chung E.J., Hossain M.T., Chung Y.R.;
RT   "Complete genome sequence of Martelella endophytica YC6887, which has
RT   antifungal activity associated with a halophyte.";
RL   Genome Announc. 3:e00366-e00366(2015).
CC   -!- FUNCTION: Shows highest activity on carboxymethyl cellulose (CMC).
CC       Shows weak activity on xylan, and no activity against avicel, laminarin
CC       and chitin. {ECO:0000269|PubMed:33523417}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC         Evidence={ECO:0000269|PubMed:33523417};
CC   -!- ACTIVITY REGULATION: Is salt tolerant and retains more than 50%
CC       residual activity up to 15% NaCl. {ECO:0000269|PubMed:33523417}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 4.5 with CMC as substrate.
CC         {ECO:0000269|PubMed:33523417};
CC       Temperature dependence:
CC         Optimum temperature is 50 degrees Celsius with CMC as substrate.
CC         Retains 50% of its activity at temperatures ranging from 30 to 60
CC         degrees Celsius. {ECO:0000269|PubMed:33523417};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000305}.
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DR   EMBL; KJ469384; AHY20451.1; -; Genomic_DNA.
DR   EMBL; CP010803; AJY45818.1; -; Genomic_DNA.
DR   SMR; A0A023VXA2; -.
DR   STRING; 1486262.TM49_09170; -.
DR   EnsemblBacteria; AJY45818; AJY45818; TM49_09170.
DR   KEGG; mey:TM49_09170; -.
DR   PATRIC; fig|1486262.3.peg.1897; -.
DR   HOGENOM; CLU_029718_0_0_5; -.
DR   Proteomes; UP000032611; Chromosome.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR018087; Glyco_hydro_5_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF00150; Cellulase; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Cellulose degradation; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..349
FT                   /note="Endoglucanase"
FT                   /id="PRO_5007368315"
FT   ACT_SITE        158
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O85465"
FT   ACT_SITE        270
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O85465"
SQ   SEQUENCE   349 AA;  37840 MW;  8CFA1D930783C9E5 CRC64;
     MPRMLAASAA IIATTLAPLS AQAAGCEMTL HGINLSGAEF GQPGDPYGQG YIYPSESTIK
     AFADDGFNAV RLPFLWERLQ PTLNGTLDAT ELSRIKDTVE TLRDNGMVVI LDVHNYARYH
     GEMIGTPNVP VAAFADFWKR LSAVFANDDD VIFGLMNEPH DISAPAWLAA ANAAIDAIRT
     IGAGNLVLVP GTAWTGAHSW SQTFYGPSNA SVMAQVVDSS NNFAYEVHQY TDDDFSGKNA
     DCSKIDDAVS ALNDFTSWLN ANDVQGFLGE FGTTEQIQCL RGLKQMVDVV QQNPRAWLGW
     AYWAGGDWWP KDSPMIIHSN PRDGGTQQLR TLQPVLGSNT TRASCNTKS