GUN_RALSO
ID GUN_RALSO Reviewed; 424 AA.
AC P58599;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Endoglucanase;
DE EC=3.2.1.4;
DE AltName: Full=Cellulase;
DE AltName: Full=Endo-1,4-beta-glucanase;
DE Flags: Precursor;
GN Name=egl; OrderedLocusNames=RSp0162; ORFNames=RS05516;
OS Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OG Plasmid megaplasmid Rsp.
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=267608;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GMI1000;
RX PubMed=11823852; DOI=10.1038/415497a;
RA Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL Nature 415:497-502(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAD17313.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL646053; CAD17313.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; P58599; -.
DR SMR; P58599; -.
DR STRING; 267608.RSp0162; -.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR EnsemblBacteria; CAD17313; CAD17313; RSp0162.
DR KEGG; rso:RSp0162; -.
DR eggNOG; COG2730; Bacteria.
DR HOGENOM; CLU_029718_0_0_4; -.
DR OMA; NEPHDIK; -.
DR Proteomes; UP000001436; Plasmid megaplasmid Rsp.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR018087; Glyco_hydro_5_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Cell membrane; Cellulose degradation; Glycosidase;
KW Hydrolase; Lipoprotein; Membrane; Palmitate; Plasmid;
KW Polysaccharide degradation; Reference proteome; Signal; Zymogen.
FT SIGNAL 1..19
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT PROPEP 20..43
FT /evidence="ECO:0000250"
FT /id="PRO_0000007867"
FT CHAIN 44..424
FT /note="Endoglucanase"
FT /id="PRO_0000007868"
FT ACT_SITE 247
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 359
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT LIPID 20
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 20
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 424 AA; 45081 MW; 5BF0D161AB7C87EF CRC64;
MHRCMPLVAA SMAALMLAGC GGGDGDTTLS TAAATDTTTL KTAATTSISP LWLTIAKDSA
AFTVSGTRTV RYGAGSAWVA KSMSGTGQCT AAFFGKDPAA GVAKVCQVAQ GTGTLLWRGV
SLAGAEFGEG SLPGTYGSNY IYPSADSATY YKNKGMNLVR LPFRWERLQP TLNQALDANE
LSRLTGFVNA VTAAGQTVLL DPHNYARYYG NVIGSSAVPN SAYADFWRRV ATQFKGNARV
IFGLMNEPNS MPTEQWLSGA NAALAAIRSA NASNVVFVPG NAWTGAHSWN QNWYGTPNGT
VMKGINDPGR NLVFEVHQYL DGDSSGQSAS CVSATIGAER LQDFTNWLRS NGYRGFLGEF
GAASNDTCNQ AVANMLTFVK NNADVWTGWA WWAGGPWWGG YMYSIEPSNG VDKPQMSVLA
PYLK
