ID   GUN_SALTI               Reviewed;         369 AA.
AC   Q8Z289;
DT   26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=Endoglucanase;
DE            EC=3.2.1.4;
DE   AltName: Full=Carboxymethylcellulase;
DE            Short=CMCase;
DE   AltName: Full=Cellulase;
DE   AltName: Full=Endo-1,4-beta-glucanase;
DE   Flags: Precursor;
GN   Name=bcsZ; OrderedLocusNames=STY4183, t3900;
OS   Salmonella typhi.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=90370;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CT18;
RX   PubMed=11677608; DOI=10.1038/35101607;
RA   Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J.,
RA   Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M.,
RA   Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A.,
RA   Davis P., Davies R.M., Dowd L., White N., Farrar J., Feltwell T.,
RA   Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A.,
RA   Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A.,
RA   Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S.,
RA   Barrell B.G.;
RT   "Complete genome sequence of a multiple drug resistant Salmonella enterica
RT   serovar Typhi CT18.";
RL   Nature 413:848-852(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700931 / Ty2;
RX   PubMed=12644504; DOI=10.1128/jb.185.7.2330-2337.2003;
RA   Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V.,
RA   Kodoyianni V., Schwartz D.C., Blattner F.R.;
RT   "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and
RT   CT18.";
RL   J. Bacteriol. 185:2330-2337(2003).
CC   -!- FUNCTION: Hydrolyzes carboxymethylcellulose.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- PATHWAY: Glycan metabolism; bacterial cellulose biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 8 (cellulase D) family.
CC       {ECO:0000305}.
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DR   EMBL; AL513382; CAD08008.1; -; Genomic_DNA.
DR   EMBL; AE014613; AAO71375.1; -; Genomic_DNA.
DR   RefSeq; NP_458303.1; NC_003198.1.
DR   RefSeq; WP_000988805.1; NZ_WSUR01000001.1.
DR   AlphaFoldDB; Q8Z289; -.
DR   SMR; Q8Z289; -.
DR   STRING; 220341.16504992; -.
DR   PRIDE; Q8Z289; -.
DR   EnsemblBacteria; AAO71375; AAO71375; t3900.
DR   KEGG; stt:t3900; -.
DR   KEGG; sty:STY4183; -.
DR   PATRIC; fig|220341.7.peg.4272; -.
DR   eggNOG; COG3405; Bacteria.
DR   HOGENOM; CLU_037297_0_0_6; -.
DR   OMA; IRVYLWV; -.
DR   UniPathway; UPA00694; -.
DR   Proteomes; UP000000541; Chromosome.
DR   Proteomes; UP000002670; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR002037; Glyco_hydro_8.
DR   InterPro; IPR019834; Glyco_hydro_8_CS.
DR   Pfam; PF01270; Glyco_hydro_8; 1.
DR   PRINTS; PR00735; GLHYDRLASE8.
DR   SUPFAM; SSF48208; SSF48208; 1.
DR   PROSITE; PS00812; GLYCOSYL_HYDROL_F8; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cellulose degradation; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..369
FT                   /note="Endoglucanase"
FT                   /id="PRO_0000007940"
FT   ACT_SITE        56
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        117
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10058"
SQ   SEQUENCE   369 AA;  41703 MW;  09954697C007F742 CRC64;
     MMTMLRGWIT MIVMLTAINA QAACSWPAWE QFKKDYISQQ GRVIDPGDAR KITTSEGQSY
     AMFFALAAND RPAFAQLFNW TQNNLAQGSL REHLPAWLWG QKDPDTWSVL DSNSASDGDI
     WMAWSLLEAG RLWKETRYTE VGTALLKRIA REEVVNVPGL GSMLLPGKIG FAEANSWRFN
     PSYLPPQLAQ YFSRFGAPWS TLRETNLRLL LETSPKGFSP DWVRYESKQG WQLKAEKTLI
     SSYDAIRVYL WTGMMHDGDP QKARLLARFK PMATLTMKNG VPPEKVDVVS GNAQGTGPVG
     FSAALLPFLQ NRGAQAVQRQ RVADHFPGSD AYYNYVLTLF GQGWDQHRFR FTVKGELLPD
     WGQECVSSR