GUX1_HUMGT
ID GUX1_HUMGT Reviewed; 525 AA.
AC P15828;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Exoglucanase 1;
DE EC=3.2.1.91;
DE AltName: Full=1,4-beta-cellobiohydrolase;
DE AltName: Full=Beta-glucancellobiohydrolase;
DE AltName: Full=Exocellobiohydrolase I;
DE AltName: Full=Exoglucanase I;
DE Flags: Precursor;
GN Name=CBH-1;
OS Humicola grisea var. thermoidea.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Chaetomiaceae; Trichocladium;
OC Trichocladium griseum.
OX NCBI_TaxID=5528;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2308855; DOI=10.1093/nar/18.3.668;
RA de Oliviera Alzevedo M., Radford A.;
RT "Sequence of cbh-1 gene of Humicola grisea var. thermoidea.";
RL Nucleic Acids Res. 18:668-668(1990).
CC -!- FUNCTION: The biological conversion of cellulose to glucose generally
CC requires three types of hydrolytic enzymes: (1) Endoglucanases which
CC cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that
CC cut the disaccharide cellobiose from the non-reducing end of the
CC cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the
CC cellobiose and other short cello-oligosaccharides to glucose.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose
CC and cellotetraose, releasing cellobiose from the non-reducing ends of
CC the chains.; EC=3.2.1.91;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 7 (cellulase C) family.
CC {ECO:0000305}.
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DR EMBL; X17258; CAA35159.1; -; Genomic_DNA.
DR PIR; S38794; S38794.
DR PDB; 4CSI; X-ray; 1.80 A; A/B=20-457.
DR PDBsum; 4CSI; -.
DR AlphaFoldDB; P15828; -.
DR SMR; P15828; -.
DR CAZy; CBM1; Carbohydrate-Binding Module Family 1.
DR CAZy; GH7; Glycoside Hydrolase Family 7.
DR BRENDA; 3.2.1.176; 8357.
DR GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR GO; GO:0016162; F:cellulose 1,4-beta-cellobiosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030248; F:cellulose binding; IEA:InterPro.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR CDD; cd07999; GH7_CBH_EG; 1.
DR Gene3D; 2.70.100.10; -; 1.
DR InterPro; IPR035971; CBD_sf.
DR InterPro; IPR000254; Cellulose-bd_dom_fun.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001722; Glyco_hydro_7.
DR InterPro; IPR037019; Glyco_hydro_7_sf.
DR PANTHER; PTHR33753; PTHR33753; 1.
DR Pfam; PF00734; CBM_1; 1.
DR Pfam; PF00840; Glyco_hydro_7; 1.
DR PRINTS; PR00734; GLHYDRLASE7.
DR SMART; SM00236; fCBD; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF57180; SSF57180; 1.
DR PROSITE; PS00562; CBM1_1; 1.
DR PROSITE; PS51164; CBM1_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Cellulose degradation;
KW Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..525
FT /note="Exoglucanase 1"
FT /id="PRO_0000007921"
FT DOMAIN 489..525
FT /note="CBM1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00597"
FT REGION 19..467
FT /note="Catalytic"
FT REGION 454..492
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 468..489
FT /note="Linker"
FT COMPBIAS 465..490
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 231
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 236
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT CARBOHYD 289
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 497..514
FT /evidence="ECO:0000250"
FT DISULFID 508..524
FT /evidence="ECO:0000250"
FT STRAND 31..38
FT /evidence="ECO:0007829|PDB:4CSI"
FT TURN 39..41
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 42..52
FT /evidence="ECO:0007829|PDB:4CSI"
FT HELIX 54..56
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 59..65
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 67..70
FT /evidence="ECO:0007829|PDB:4CSI"
FT TURN 76..78
FT /evidence="ECO:0007829|PDB:4CSI"
FT HELIX 82..88
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 89..91
FT /evidence="ECO:0007829|PDB:4CSI"
FT HELIX 96..100
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 102..105
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 108..116
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 121..123
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 125..136
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 144..151
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 159..166
FT /evidence="ECO:0007829|PDB:4CSI"
FT TURN 170..176
FT /evidence="ECO:0007829|PDB:4CSI"
FT HELIX 184..186
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 200..202
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 224..228
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 231..237
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 242..247
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 249..251
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 255..258
FT /evidence="ECO:0007829|PDB:4CSI"
FT HELIX 259..262
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 265..267
FT /evidence="ECO:0007829|PDB:4CSI"
FT TURN 268..271
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 273..275
FT /evidence="ECO:0007829|PDB:4CSI"
FT TURN 284..288
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 292..294
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 297..300
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 305..313
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 317..328
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 331..334
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 346..348
FT /evidence="ECO:0007829|PDB:4CSI"
FT HELIX 350..360
FT /evidence="ECO:0007829|PDB:4CSI"
FT HELIX 365..368
FT /evidence="ECO:0007829|PDB:4CSI"
FT HELIX 371..380
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 383..391
FT /evidence="ECO:0007829|PDB:4CSI"
FT TURN 393..397
FT /evidence="ECO:0007829|PDB:4CSI"
FT HELIX 398..401
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 402..405
FT /evidence="ECO:0007829|PDB:4CSI"
FT HELIX 406..408
FT /evidence="ECO:0007829|PDB:4CSI"
FT TURN 412..414
FT /evidence="ECO:0007829|PDB:4CSI"
FT HELIX 426..432
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 437..447
FT /evidence="ECO:0007829|PDB:4CSI"
FT STRAND 450..452
FT /evidence="ECO:0007829|PDB:4CSI"
SQ SEQUENCE 525 AA; 55694 MW; A6684D4CF881E090 CRC64;
MRTAKFATLA ALVASAAAQQ ACSLTTERHP SLSWNKCTAG GQCQTVQASI TLDSNWRWTH
QVSGSTNCYT GNKWDTSICT DAKSCAQNCC VDGADYTSTY GITTNGDSLS LKFVTKGQHS
TNVGSRTYLM DGEDKYQTFE LLGNEFTFDV DVSNIGCGLN GALYFVSMDA DGGLSRYPGN
KAGAKYGTGY CDAQCPRDIK FINGEANIEG WTGSTNDPNA GAGRYGTCCS EMDIWEANNM
ATAFTPHPCT IIGQSRCEGD SCGGTYSNER YAGVCDPDGC DFNSYRQGNK TFYGKGMTVD
TTKKITVVTQ FLKDANGDLG EIKRFYVQDG KIIPNSESTI PGVEGNSITQ DWCDRQKVAF
GDIDDFNRKG GMKQMGKALA GPMVLVMSIW DDHASNMLWL DSTFPVDAAG KPGAERGACP
TTSGVPAEVE AEAPNSNVVF SNIRFGPIGS TVAGLPGAGN GGNNGGNPPP PTTTTSSAPA
TTTTASAGPK AGRWQQCGGI GFTGPTQCEE PYICTKLNDW YSQCL
