AMTN_RAT
ID AMTN_RAT Reviewed; 212 AA.
AC Q3HS82;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Amelotin;
DE Flags: Precursor;
GN Name=Amtn {ECO:0000312|EMBL:CAJ77764.1}; ORFNames=EO-017;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), GLYCOSYLATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND FUNCTION.
RC STRAIN=Wistar {ECO:0000269|PubMed:16787391};
RC TISSUE=Enamel organ {ECO:0000269|PubMed:16787391};
RX PubMed=16787391; DOI=10.1042/bj20060662;
RA Moffatt P., Smith C.E., St Arnaud R., Simmons D., Wright J.T., Nanci A.;
RT "Cloning of rat amelotin and localization of the protein to the basal
RT lamina of maturation stage ameloblasts and junctional epithelium.";
RL Biochem. J. 399:37-46(2006).
RN [2] {ECO:0000305, ECO:0000312|EMBL:CAJ77764.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=Wistar {ECO:0000312|EMBL:CAJ77764.1};
RC TISSUE=Incisor {ECO:0000312|EMBL:CAJ77764.1};
RX PubMed=17143547;
RA Trueb B., Taeschler S., Schild C., Lang N.P.;
RT "Expression of phosphoproteins and amelotin in teeth.";
RL Int. J. Mol. Med. 19:49-54(2007).
CC -!- FUNCTION: Is a promoter of calcium phosphate mineralization, playing a
CC critical role in the formation of the compact, mineralized, aprismatic
CC enamel surface layer during the maturation stage of amelogenesis.
CC {ECO:0000250|UniProtKB:Q9D3J8, ECO:0000269|PubMed:16787391}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9D3J8}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1 {ECO:0000269|PubMed:16787391};
CC IsoId=Q3HS82-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|PubMed:16787391};
CC IsoId=Q3HS82-2; Sequence=VSP_052355;
CC Name=3 {ECO:0000269|PubMed:16787391};
CC IsoId=Q3HS82-3; Sequence=VSP_052354;
CC -!- TISSUE SPECIFICITY: Highest expression in the mandible. Found in the
CC basal lamina of maturation stage ameloblasts of incisors and unerupted
CC molars. Also found in the internal basal lamina of junctional
CC epithelium in molars. {ECO:0000269|PubMed:16787391}.
CC -!- DEVELOPMENTAL STAGE: Expressed in ameloblasts as they undergo post-
CC secretory transition. Expression decreases as ameloblasts progress
CC through maturation. {ECO:0000269|PubMed:16787391}.
CC -!- PTM: O-glycosylated. {ECO:0000305|PubMed:16787391}.
CC -!- PTM: Phosphorylated by FAM20C in vitro. {ECO:0000250|UniProtKB:Q6UX39}.
CC -!- SIMILARITY: Belongs to the amelotin family. {ECO:0000305}.
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DR EMBL; DQ198381; ABA54405.1; -; mRNA.
DR EMBL; AM231717; CAJ77764.1; -; mRNA.
DR RefSeq; NP_001037761.1; NM_001044296.1. [Q3HS82-1]
DR RefSeq; XP_017454872.1; XM_017599383.1. [Q3HS82-1]
DR AlphaFoldDB; Q3HS82; -.
DR STRING; 10116.ENSRNOP00000058805; -.
DR PaxDb; Q3HS82; -.
DR Ensembl; ENSRNOT00000065778; ENSRNOP00000058805; ENSRNOG00000003776. [Q3HS82-1]
DR GeneID; 689404; -.
DR KEGG; rno:689404; -.
DR UCSC; RGD:1592356; rat. [Q3HS82-1]
DR CTD; 401138; -.
DR RGD; 1592356; Amtn.
DR eggNOG; ENOG502SFV7; Eukaryota.
DR GeneTree; ENSGT00390000006715; -.
DR HOGENOM; CLU_082745_0_0_1; -.
DR InParanoid; Q3HS82; -.
DR OMA; PQMLPIF; -.
DR OrthoDB; 1353258at2759; -.
DR PhylomeDB; Q3HS82; -.
DR TreeFam; TF337677; -.
DR Reactome; R-RNO-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR Reactome; R-RNO-8957275; Post-translational protein phosphorylation.
DR PRO; PR:Q3HS82; -.
DR Proteomes; UP000002494; Chromosome 14.
DR Bgee; ENSRNOG00000003776; Expressed in lung and 4 other tissues.
DR GO; GO:0005604; C:basement membrane; IDA:HGNC-UCL.
DR GO; GO:0005911; C:cell-cell junction; IDA:HGNC-UCL.
DR GO; GO:0031012; C:extracellular matrix; IDA:HGNC-UCL.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0031214; P:biomineral tissue development; IEA:UniProtKB-KW.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; IEP:HGNC-UCL.
DR GO; GO:0070169; P:positive regulation of biomineral tissue development; ISO:RGD.
DR GO; GO:0070175; P:positive regulation of enamel mineralization; ISS:UniProtKB.
DR InterPro; IPR031501; Amelotin.
DR PANTHER; PTHR36858; PTHR36858; 1.
DR Pfam; PF15757; Amelotin; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Biomineralization; Cell adhesion; Glycoprotein;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000250|UniProtKB:Q6UX39"
FT CHAIN 17..212
FT /note="Amelotin"
FT /evidence="ECO:0000250|UniProtKB:Q6UX39"
FT /id="PRO_5000140668"
FT REGION 23..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 141..212
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..177
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 192..212
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 19..119
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16787391"
FT /id="VSP_052354"
FT VAR_SEQ 111..119
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16787391"
FT /id="VSP_052355"
SQ SEQUENCE 212 AA; 22080 MW; ED94D55BC1B849EE CRC64;
MKTVVLLLCL LGSAQSLPRQ LSPALGAPAT KPTPGQVTPL TQQQPNQVFP SISLIPLTQL
LTLGSDLPLF NPATMPHGTQ TLPFTLGPLN GQQQLQPQML PIIVAQLGAQ GALLSSEELP
LASQIFTGLL IHPLFPGAIQ PSGQTGAKPD VQNGALPTRQ AGASPANQAT TPGHTTPAVT
DDDDYEMSTP AGLQRATHTT EGTTMDPPNR TK
