ID   AMTR1_ALTAL             Reviewed;         770 AA.
AC   C9K7C2;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Transcription activator AMTR1 {ECO:0000303|PubMed:17990954};
DE   AltName: Full=AM-toxin biosynthesis regulator 1 {ECO:0000303|PubMed:17990954};
GN   Name=AMTR1 {ECO:0000303|PubMed:17990954};
OS   Alternaria alternata (Alternaria rot fungus) (Torula alternata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Alternaria;
OC   Alternaria sect. Alternaria; Alternaria alternata complex.
OX   NCBI_TaxID=5599;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=NBRC 8984;
RX   PubMed=17990954; DOI=10.1094/mpmi-20-12-1463;
RA   Harimoto Y., Hatta R., Kodama M., Yamamoto M., Otani H., Tsuge T.;
RT   "Expression profiles of genes encoded by the supernumerary chromosome
RT   controlling AM-toxin biosynthesis and pathogenicity in the apple pathotype
RT   of Alternaria alternata.";
RL   Mol. Plant Microbe Interact. 20:1463-1476(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NBRC 8984;
RA   Harimoto Y., Kodama M., Yamamoto M., Otani H., Tsuge T.;
RT   "A Zn(II)2Cys6 transcription regulator encoded by the AMT gene cluster
RT   negatively controls AM-toxin production in the apple pathotype of
RT   Alternaria alternata.";
RL   Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   REVIEW ON HOST-SELECTIVE TOXINS.
RX   PubMed=22846083; DOI=10.1111/j.1574-6976.2012.00350.x;
RA   Tsuge T., Harimoto Y., Akimitsu K., Ohtani K., Kodama M., Akagi Y.,
RA   Egusa M., Yamamoto M., Otani H.;
RT   "Host-selective toxins produced by the plant pathogenic fungus Alternaria
RT   alternata.";
RL   FEMS Microbiol. Rev. 37:44-66(2013).
CC   -!- FUNCTION: Transcription factor that regulates the expression of the
CC       gene clusters that mediate the biosynthesis of AM-toxins, host-
CC       selective toxins (HSTs) causing Alternaria blotch on apple, a worldwide
CC       distributed disease (Probable). AM-toxins have two target sites for
CC       affecting susceptible apple cells; they cause invagination of the
CC       plasma membrane and electrolyte loss and chloroplast disorganization
CC       (PubMed:22846083). {ECO:0000303|PubMed:22846083,
CC       ECO:0000305|PubMed:17990954}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- INDUCTION: Expression is up-regulated more than 10 fold in toxin
CC       producing cultures. {ECO:0000269|PubMed:17990954}.
CC   -!- MISCELLANEOUS: Gene clusters encoding host-selective toxins (HSTs) are
CC       localized on conditionally dispensable chromosomes (CDCs), also called
CC       supernumerary chromosomes, where they are present in multiple copies
CC       (PubMed:17990954). The CDCs are not essential for saprophytic growth
CC       but controls host-selective pathogenicity (PubMed:17990954).
CC       {ECO:0000269|PubMed:17990954}.
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DR   EMBL; AB525198; BAI44746.1; -; Genomic_DNA.
DR   AlphaFoldDB; C9K7C2; -.
DR   SMR; C9K7C2; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR021858; Fun_TF.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF11951; Fungal_trans_2; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Metal-binding; Nucleus; Transcription;
KW   Transcription regulation; Virulence; Zinc.
FT   CHAIN           1..770
FT                   /note="Transcription activator AMTR1"
FT                   /id="PRO_0000444837"
FT   DNA_BIND        7..34
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
SQ   SEQUENCE   770 AA;  86573 MW;  2980F0E533DB0C03 CRC64;
     MRSRTGCLTC RQRKLKCDEK KPVCRQCAKA SRECIPSPGI VFRHQQNASM NGEDSTAEKS
     LKGFYAYKNT FDKDAMWVDI PKCITFVTTT NLYLDSGVLE LDTMLATSME SPKPFEPHCL
     ASWRTQGDRH PIGNVPSSLR SELAPSSVSC CLTQDVKALS PQIHSSPGSV IERPVSPPMS
     LFNPHIGLIM NLTTSSAMPS TDSFFSLPFD STDYMPRPVS RPRLRWRPPR PNASILTQDE
     HEIACLLQWF SEGPGYWMDL FITGTYFASH VPVEAVENPL LKYAIAACAA KAFARVQDQK
     PAMGGSSTRQ AGMKHYPNVP LVDWEHKTAV YYNTTVSMLL QALNGKVASS PNGSKCELRQ
     RNGDPACAYN VSAPKPRRIS QNTSFVPSTE ELLVVIAILC FYEFLDTSIS EWEKHLHGAK
     SFLVLSQQHI RSLRLPNLAS PMFPTSSKFA SKAWRAVFWN IARQDMLAAF INKTSTRLDT
     DDLTLWREAG LILDEQACIM PSNAAVYGYL EEGDAMIKEE LICNTLVWLM VKLVNFMAVG
     SVIPSRSGAA WDGVVHRTRF CQWFSLRKQF QVWHEGLPVT FRPSARVAPS HTSGQVSNDD
     SASMFSEAWH SMPMCASTMQ TYYMSQILLF MNKPHESTLE CHTELIRMSY HQSVLAACQI
     HSRRIIGISL AQPDKAVRVH SVQPLFTAGQ CLSDNRECQI VLRLLRDIES DTGWATDYRV
     QQLVEQWQRN EPDSQALWSD SRCAIPHENK YPSPTFNTVR LQHGSDLRSN