ID   AMT_AQUAE               Reviewed;         423 AA.
AC   O66515;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Ammonium transporter {ECO:0000250|UniProtKB:P69681};
DE   AltName: Full=Ammonia channel {ECO:0000250|UniProtKB:P69681};
DE   AltName: Full=Ammonium channel {ECO:0000250|UniProtKB:P69681};
DE   Flags: Precursor;
GN   Name=amt; Synonyms=amtB; OrderedLocusNames=aq_112;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
RN   [2]
RP   PROTEIN SEQUENCE OF N-TERMINUS, AND CRYSTALLIZATION.
RX   PubMed=15361618; DOI=10.1126/science.1101952;
RA   Khademi S., O'Connell J.D. III, Remis J., Robles-Colmenares Y.,
RA   Miercke L.J., Stroud R.M.;
RT   "Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35
RT   A.";
RL   Science 305:1587-1594(2004).
CC   -!- FUNCTION: Involved in the uptake of ammonium/ammonia (NH(4)(+)/NH(3)).
CC       {ECO:0000250|UniProtKB:P69681}.
CC   -!- SUBUNIT: Homotrimer. {ECO:0000250|UniProtKB:P69681}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC       family. {ECO:0000305}.
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DR   EMBL; AE000657; AAC06478.1; -; Genomic_DNA.
DR   PIR; H70310; H70310.
DR   RefSeq; NP_213075.1; NC_000918.1.
DR   RefSeq; WP_010880013.1; NC_000918.1.
DR   AlphaFoldDB; O66515; -.
DR   SMR; O66515; -.
DR   STRING; 224324.aq_112; -.
DR   TCDB; 1.A.11.1.5; the ammonium transporter channel (amt) family.
DR   EnsemblBacteria; AAC06478; AAC06478; aq_112.
DR   KEGG; aae:aq_112; -.
DR   PATRIC; fig|224324.8.peg.96; -.
DR   eggNOG; COG0004; Bacteria.
DR   HOGENOM; CLU_000445_33_0_0; -.
DR   InParanoid; O66515; -.
DR   OMA; MACWNSN; -.
DR   OrthoDB; 562287at2; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0008519; F:ammonium transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0072488; P:ammonium transmembrane transport; IBA:GO_Central.
DR   Gene3D; 1.10.3430.10; -; 1.
DR   InterPro; IPR029020; Ammonium/urea_transptr.
DR   InterPro; IPR001905; Ammonium_transpt.
DR   InterPro; IPR018047; Ammonium_transpt_CS.
DR   InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR   PANTHER; PTHR43029; PTHR43029; 1.
DR   Pfam; PF00909; Ammonium_transp; 1.
DR   TIGRFAMs; TIGR00836; amt; 1.
DR   PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE   1: Evidence at protein level;
KW   Ammonia transport; Cell membrane; Direct protein sequencing; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000269|PubMed:15361618"
FT   CHAIN           21..423
FT                   /note="Ammonium transporter"
FT                   /id="PRO_0000001306"
FT   TRANSMEM        31..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        183..203
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        217..237
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        278..298
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        301..321
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        331..351
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        374..394
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   423 AA;  44323 MW;  166203BBB2A8A15B CRC64;
     MRALGFIGII LSIFSSFAYA SEAKLDTGNT AWMLVASALV VFMTVPGLAL FYGGLDKSKS
     ILNTIAMSFS AFAVVTLTWI FVGYSVAYGD DIFGFIGNPF QYVLGKGISG INSDTGYPAL
     LDLMFQLTFA TITTALISGS FVGRMKFSAW ILFAILWSVF VYPPVAHWVW GGGFLANDGA
     LDFAGGTVVH INAGIAGLVG ALILGRRKDT SLIPNNVPLV ALGAGILWFG WFGFNAGSAL
     GANESAAWAM INTTVATSTA ALAWMFTEWL HVGKPTVVGI SSGIVAGLVA ITPAAGFVNL
     IGSIFIGAIA SVCAYFMVAL VKPKFGYDDA LDVFGIHGVR GIVGAVLTGV FADPNVGGTP
     GLLYGNPKQV LIQIEGVIAT ILYSAILTAV ILLVLKAVVG LRVSEEEELE LDSSLHGEKA
     YNL