GW6A_ORYSJ
ID GW6A_ORYSJ Reviewed; 419 AA.
AC Q67UR2; A0A0A8JXA3;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Acetyl transferase GW6a {ECO:0000305};
DE EC=2.3.1.- {ECO:0000269|PubMed:25535376};
DE AltName: Full=GNAT-like histone acetyltransferase 1 {ECO:0000303|PubMed:25535376};
DE Short=OsglHAT1 {ECO:0000303|PubMed:25535376};
DE AltName: Full=Histone H4 acetyltransferase {ECO:0000305};
DE AltName: Full=Protein GRAIN WEIGHT ON CHROMOSOME 6 {ECO:0000303|PubMed:25535376};
GN Name=GW6A {ECO:0000303|PubMed:25535376};
GN Synonyms=GNAT1 {ECO:0000303|PubMed:33970916},
GN HAT1 {ECO:0000303|PubMed:25535376};
GN OrderedLocusNames=Os06g0650300 {ECO:0000312|EMBL:BAS98897.1},
GN LOC_Os06g44100 {ECO:0000305};
GN ORFNames=P0453H04.20 {ECO:0000312|EMBL:BAD38107.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF ARG-146.
RX PubMed=25535376; DOI=10.1073/pnas.1421127112;
RA Song X.J., Kuroha T., Ayano M., Furuta T., Nagai K., Komeda N., Segami S.,
RA Miura K., Ogawa D., Kamura T., Suzuki T., Higashiyama T., Yamasaki M.,
RA Mori H., Inukai Y., Wu J., Kitano H., Sakakibara H., Jacobsen S.E.,
RA Ashikari M.;
RT "Rare allele of a previously unidentified histone H4 acetyltransferase
RT enhances grain weight, yield, and plant biomass in rice.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:76-81(2015).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=33970916; DOI=10.1371/journal.pgen.1009292;
RA Hibara K.I., Miya M., Benvenuto S.A., Hibara-Matsuo N., Mimura M.,
RA Yoshikawa T., Suzuki M., Kusaba M., Taketa S., Itoh J.I.;
RT "Regulation of the plastochron by three many-noded dwarf genes in barley.";
RL PLoS Genet. 17:E1009292-E1009292(2021).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP FUNCTION, INTERACTION WITH HDR3, AND UBIQUITINATION AT LYS-63.
RX PubMed=34323980; DOI=10.1093/plcell/koab194;
RA Gao Q., Zhang N., Wang W.Q., Shen S.Y., Bai C., Song X.J.;
RT "The ubiquitin-interacting motif-type ubiquitin receptor HDR3 interacts
RT with and stabilizes the histone acetyltransferase GW6a to control the grain
RT size in rice.";
RL Plant Cell 33:3331-3347(2021).
CC -!- FUNCTION: Possesses intrinsic histone acetyltransferase activity and
CC acts as positive regulator of grain weight, hull size, yield, and plant
CC biomass (PubMed:25535376, PubMed:34323980). Regulates postitively grain
CC weight and yield by enlarging spikelet hulls via increasing cell number
CC and accelerating grain filling (PubMed:25535376). In vitro, catalyzes
CC the acetylation of histone H4 at Lys-6 (H4K5ac), Lys-13 (H4K12ac) and
CC Lys-17 (H4K16ac) (PubMed:25535376). Involved in the regulation of
CC plastochron (the time interval between leaf initiation event)
CC (PubMed:33970916). {ECO:0000269|PubMed:25535376,
CC ECO:0000269|PubMed:33970916, ECO:0000269|PubMed:34323980}.
CC -!- SUBUNIT: Interacts (via C-terminus) with HDR3 (via N-terminus).
CC {ECO:0000269|PubMed:34323980}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25535376}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaf blades, leaf sheaths,
CC shoot apical meristem and young panicles.
CC {ECO:0000269|PubMed:25535376}.
CC -!- DEVELOPMENTAL STAGE: Expressed at the basal part of the abaxial side of
CC leaves in the vegetative phase (PubMed:25535376). In the reproductive
CC phase, expressed in the bracts of initiating branches
CC (PubMed:25535376). {ECO:0000269|PubMed:25535376}.
CC -!- PTM: Ubiquitinated at Lys-63 by HDR3 (PubMed:34323980).
CC Polyubiquitination of GW6A delays its degradation by the 26S proteasome
CC and enhances GW6A histone acetyltransferase activity (PubMed:34323980).
CC {ECO:0000269|PubMed:34323980}.
CC -!- MISCELLANEOUS: Over-expression of GWA6A enhances grain weight and yield
CC by enlarging spikelet hulls via increasing cell number and accelerating
CC grain filling, and increases global acetylation levels of histone H4.
CC {ECO:0000269|PubMed:25535376}.
CC -!- SIMILARITY: Belongs to the acetyltransferase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LC003015; BAQ15250.1; -; mRNA.
DR EMBL; LC003016; BAQ15251.1; -; Genomic_DNA.
DR EMBL; LC593242; BCN13394.1; -; mRNA.
DR EMBL; AP005453; BAD38107.1; -; Genomic_DNA.
DR EMBL; AP014962; BAS98897.1; -; Genomic_DNA.
DR RefSeq; XP_015642751.1; XM_015787265.1.
DR AlphaFoldDB; Q67UR2; -.
DR STRING; 4530.OS06T0650300-00; -.
DR PaxDb; Q67UR2; -.
DR PRIDE; Q67UR2; -.
DR EnsemblPlants; Os06t0650300-01; Os06t0650300-01; Os06g0650300.
DR GeneID; 107275998; -.
DR Gramene; Os06t0650300-01; Os06t0650300-01; Os06g0650300.
DR KEGG; osa:107275998; -.
DR eggNOG; ENOG502RFA7; Eukaryota.
DR HOGENOM; CLU_057213_0_0_1; -.
DR InParanoid; Q67UR2; -.
DR OrthoDB; 567615at2759; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR GO; GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR InterPro; IPR000182; GNAT_dom.
DR Pfam; PF00583; Acetyltransf_1; 1.
DR SUPFAM; SSF55729; SSF55729; 1.
DR PROSITE; PS51186; GNAT; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Nucleus; Reference proteome; Transferase; Ubl conjugation.
FT CHAIN 1..419
FT /note="Acetyl transferase GW6a"
FT /id="PRO_0000454922"
FT DOMAIN 12..210
FT /note="N-acetyltransferase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
FT REGION 44..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..59
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 146
FT /note="R->W: Abolishes histone acetyltransferase activity
FT in vitro."
FT /evidence="ECO:0000269|PubMed:25535376"
SQ SEQUENCE 419 AA; 45696 MW; B125377DF01D82C3 CRC64;
MVETTTMMKV LVRVREFDVE KDLPAVEELE RRCQVGLSGD MAAVHDHADD GDGAAAKEKK
KTKTKTKKKK ASMSLCVEQI GDPLARVRHA PEHVMLVAEY GEEEEKKKVV GVIKACVKTV
SRGGKQEKPF VKVANLLGLR VSPSHRRLGI GTALVRRAEE WCVARGAEHA TMATTESNAA
SLALFTGRFG YAPFRRPEFI GHPVHAHRLP VARGHRVFQL PPEVAAAAYA RLLPPQDAEF
LPADMPALLA HKLTLGTFVA VAADGASFAV LSVWDSTRSL SLRVSGAPAL LRASLAALRA
LDRGAPWLHL PSIPDIFRPF GAYLLYGLRM SGPDGPALLR SLCHHAHNVA RKNPACAVVA
ADISPDDPAA AAVPRWRRFC CDEDVWCIKN LNPDEHDADD WAAPPPPPGR HLFVDPREF
