GXCB_DICDI
ID GXCB_DICDI Reviewed; 1198 AA.
AC Q55E26; Q2MCX4;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Rac guanine nucleotide exchange factor B;
GN Name=gxcB; Synonyms=Trix; ORFNames=DDB_G0269424;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=AX2;
RX PubMed=16781009; DOI=10.1016/j.ejcb.2006.05.005;
RA Strehle A., Schleicher M., Faix J.;
RT "Trix, a novel Rac guanine-nucleotide exchange factor from Dictyostelium
RT discoideum is an actin-binding protein and accumulates at endosomes.";
RL Eur. J. Cell Biol. 85:1035-1045(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Involved in the regulation of the late steps of the endocytic
CC pathway. {ECO:0000269|PubMed:16781009}.
CC -!- SUBUNIT: Binds to F-actin.
CC -!- SUBCELLULAR LOCATION: Late endosome {ECO:0000269|PubMed:16781009}.
CC -!- DEVELOPMENTAL STAGE: Expressed during vegetative growth and early
CC stages of development. {ECO:0000269|PubMed:16781009}.
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DR EMBL; AM181592; CAJ57479.1; -; mRNA.
DR EMBL; AAFI02000005; EAL72062.1; -; Genomic_DNA.
DR RefSeq; XP_645955.1; XM_640863.1.
DR AlphaFoldDB; Q55E26; -.
DR SMR; Q55E26; -.
DR STRING; 44689.DDB0233173; -.
DR PaxDb; Q55E26; -.
DR PRIDE; Q55E26; -.
DR EnsemblProtists; EAL72062; EAL72062; DDB_G0269424.
DR GeneID; 8616899; -.
DR KEGG; ddi:DDB_G0269424; -.
DR dictyBase; DDB_G0269424; gxcB.
DR eggNOG; KOG2046; Eukaryota.
DR eggNOG; KOG3522; Eukaryota.
DR HOGENOM; CLU_271096_0_0_1; -.
DR InParanoid; Q55E26; -.
DR OMA; LYSYLIM; -.
DR PRO; PR:Q55E26; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005884; C:actin filament; IBA:GO_Central.
DR GO; GO:0005938; C:cell cortex; IDA:dictyBase.
DR GO; GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0051015; F:actin filament binding; IDA:dictyBase.
DR GO; GO:0008093; F:cytoskeletal anchor activity; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0051764; P:actin crosslink formation; IDA:dictyBase.
DR GO; GO:0051017; P:actin filament bundle assembly; IDA:dictyBase.
DR GO; GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
DR GO; GO:0006887; P:exocytosis; IMP:dictyBase.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR CDD; cd00014; CH; 2.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 1.10.418.10; -; 3.
DR Gene3D; 1.20.900.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR001331; GDS_CDC24_CS.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR003096; SM22_calponin.
DR Pfam; PF00307; CH; 3.
DR Pfam; PF00621; RhoGEF; 1.
DR PRINTS; PR00888; SM22CALPONIN.
DR SMART; SM00033; CH; 3.
DR SMART; SM00233; PH; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SUPFAM; SSF47576; SSF47576; 2.
DR SUPFAM; SSF48065; SSF48065; 1.
DR PROSITE; PS50021; CH; 3.
DR PROSITE; PS00741; DH_1; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Endocytosis; Endosome; Guanine-nucleotide releasing factor;
KW Reference proteome; Repeat.
FT CHAIN 1..1198
FT /note="Rac guanine nucleotide exchange factor B"
FT /id="PRO_0000328627"
FT DOMAIN 126..232
FT /note="Calponin-homology (CH) 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT DOMAIN 341..449
FT /note="Calponin-homology (CH) 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT DOMAIN 511..619
FT /note="Calponin-homology (CH) 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT DOMAIN 632..846
FT /note="DH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00062"
FT DOMAIN 876..1026
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 1..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 260..284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 940..989
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1076..1198
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..21
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..104
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 940..954
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 963..989
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1076..1122
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1123..1157
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1198 AA; 134479 MW; FF31F4B5E94F0FEB CRC64;
MFSNFFGSSK RNTIASSSSS SKKDKDNGKD ESSKLKNSGS STLPKPITNN ESGNNFITSP
SVSSPLISPL SSSPSPLLSS SSNSIQSTSH QQQQQHQGVI TSLQMKPSCT SLTDDIEKKK
QAKYDSSLEQ TARKWVCDVL EIQLEDDKTF YELFKNGVLL CRLINKLRGG TIKRINESTI
SFKQLENIEN YLKACKTLGL QSVNLFNSID LHENKDISLV ITNIVVLGKH ASKIEGYNGI
HLAGERKIIK ITTTPVSPLF GGNHNNNNNN NNNNNTSNGD LSPVSITSAS GGSSNYNSYS
NNPLNKSSNG KKAKWRKSVK IPAVSSLNSD IRTKEAFKFS PELQKAAQDW IEEVTKEKFK
LPSFSSSLKD GILLCRVINT IIPNTILYIN NGNSSFKKME NIGNYLKGCL VVGLKKTDLF
DTPDLFEEKN INFVISNIHV LGNHVNKMYS HLKLPLIKNI GNGGNGGNGG IGGGGGSDGS
PLKMYSSIIH SKFGNGSSNT SSGNGGVIIP PEDMKDLKDW INHHLRAHHS LQIGSDMSND
LRNGVTLLTL LEELTLQKVG IFAREPVLPW HFMQNICLLL NFLRENSVHN VSDISPHDLF
NGDIHSLCMI TRLIRENFDT DHQMKSIPMD TRRQKVIEEI IATEQSYVKS LSTVYNLLIV
PLLNSLDTNS PILSNDEISS IFGNWEHLLR SHINLLKEFK LKLNLPFNDL TIDDSNQNQN
HNNIFGDSIF DSNITIGDVF LEKCEFLKDN YTNYINNYDN SYQRVKRLKK SNSNFEELVN
TFEIFQDTHN GLDLYSYLIM PIQRIVRYIL LLKEVIKYTP STHPDYQMLQ NAKENIKRVA
DHVNESKMAV ENKRKILSIQ DSIQNLQFNL MDKERTYIRE GFLEIEDTFK KDSYFFLFSD
LLLFVKYKPS EETGKEFKYK EVFYLDQVVD VSDILSDDEG EACVDGDDDG GEYEGGNGDA
TSGSADPEDQ TLRRSCNSNN NNNSNSNKSN TVYSFEIETC EFSLVLLAES HTEKIEWMED
LRSCLQHQLI KEEDQLSSLS LSDNDDDNDA DADVESSLNS ISSPLLLLNN NNIINNNNNN
NNNNNNNNNN NNNNNNCKNS NININSSIDN NSDNNNNDYD SKINSEENGD SSDEENKTSN
RRSVSFKTHK RLESDETISD TESDDYELVC GRSKKSQPPP VPPRKITFSD TIKNIDNQ
