GYP6_YEAST
ID GYP6_YEAST Reviewed; 458 AA.
AC P32806; D6VWE0;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=GTPase-activating protein GYP6;
DE AltName: Full=GAP for YPT6;
GN Name=GYP6; OrderedLocusNames=YJL044C; ORFNames=J1202;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8441469; DOI=10.1038/361736a0;
RA Strom M., Vollmer P., Tan T.J., Gallwitz D.;
RT "A yeast GTPase-activating protein that interacts specifically with a
RT member of the Ypt/Rab family.";
RL Nature 361:736-739(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-436, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Stimulates specifically the GTPase activity of YPT6.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 2990 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X68506; CAA48518.1; -; Genomic_DNA.
DR EMBL; Z49319; CAA89335.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08756.1; -; Genomic_DNA.
DR PIR; S56816; S56816.
DR RefSeq; NP_012491.3; NM_001181477.3.
DR AlphaFoldDB; P32806; -.
DR SMR; P32806; -.
DR BioGRID; 33714; 125.
DR DIP; DIP-3973N; -.
DR IntAct; P32806; 6.
DR MINT; P32806; -.
DR STRING; 4932.YJL044C; -.
DR iPTMnet; P32806; -.
DR SWISS-2DPAGE; P32806; -.
DR MaxQB; P32806; -.
DR PaxDb; P32806; -.
DR PRIDE; P32806; -.
DR EnsemblFungi; YJL044C_mRNA; YJL044C; YJL044C.
DR GeneID; 853406; -.
DR KEGG; sce:YJL044C; -.
DR SGD; S000003580; GYP6.
DR VEuPathDB; FungiDB:YJL044C; -.
DR eggNOG; KOG1091; Eukaryota.
DR HOGENOM; CLU_033840_0_0_1; -.
DR InParanoid; P32806; -.
DR OMA; IWLIRWT; -.
DR BioCyc; YEAST:G3O-31509-MON; -.
DR PRO; PR:P32806; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P32806; protein.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0005768; C:endosome; IDA:SGD.
DR GO; GO:0005802; C:trans-Golgi network; IDA:SGD.
DR GO; GO:0005096; F:GTPase activator activity; IDA:SGD.
DR GO; GO:0090630; P:activation of GTPase activity; IDA:SGD.
DR InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF47923; SSF47923; 2.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; GTPase activation; Phosphoprotein; Reference proteome.
FT CHAIN 1..458
FT /note="GTPase-activating protein GYP6"
FT /id="PRO_0000208013"
FT DOMAIN 33..316
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT REGION 116..136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 436
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT CONFLICT 413
FT /note="F -> L (in Ref. 1; CAA48518)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 458 AA; 53815 MW; DDC21820E00EE06C CRC64;
MKDVLQWAID HYETRDQLVQ KGIWKGELYH DSIFKENSRG WLWKALLLCD ENNNCLLTDN
FKGLDLNQFG LVPVPMLADG DNYDENHNAN VPKRVLHSNV SSSVGIRRLT PVEAVEKHPL
SDDNDKTKGS LSKGSDEKPL TLRETLEIID LDLSRIMLDD IFQEPKVHAQ MRQLLYNYLL
IHQSEHLQYK QGFHEILSVI YLQLYHGTDL DNTDLQNVLI IFNKLMNQIE PIFYNEENLI
NWDKRVFTKI FRICLPDLFS KVFYQPPKTG SGKKKNVDHL IHSNLIWLIR WTRLLFLREL
PLKYVLIVWD HVLTFNYPLD IFIACTIITL LLSIYDELHE LVSQGDYEHT NNNDEFVELI
LHFKKIFEKE DASKDDEKFL DLCKVTGNLC ELWYGKNYDD MRLICDTFIN AKFGIKTSDV
LSMETAKLTI DPNRQSLENK LRERVRQTIL KNKKKISQ
