ID   GYP8_YEAST              Reviewed;         497 AA.
AC   P43570; D6VTK3;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 146.
DE   RecName: Full=GTPase-activating protein GYP8;
GN   Name=GYP8; OrderedLocusNames=YFL027C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7670463; DOI=10.1038/ng0795-261;
RA   Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA   Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA   Eki T.;
RT   "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT   cerevisiae.";
RL   Nat. Genet. 10:261-268(1995).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- MISCELLANEOUS: Present with 573 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; D50617; BAA09211.1; -; Genomic_DNA.
DR   EMBL; AY693137; AAT93156.1; -; Genomic_DNA.
DR   EMBL; BK006940; DAA12413.1; -; Genomic_DNA.
DR   PIR; S56227; S56227.
DR   RefSeq; NP_444296.1; NM_001179939.1.
DR   AlphaFoldDB; P43570; -.
DR   SMR; P43570; -.
DR   BioGRID; 31119; 91.
DR   DIP; DIP-5431N; -.
DR   IntAct; P43570; 1.
DR   STRING; 4932.YFL027C; -.
DR   MaxQB; P43570; -.
DR   PaxDb; P43570; -.
DR   PRIDE; P43570; -.
DR   EnsemblFungi; YFL027C_mRNA; YFL027C; YFL027C.
DR   GeneID; 850517; -.
DR   KEGG; sce:YFL027C; -.
DR   SGD; S000001867; GYP8.
DR   VEuPathDB; FungiDB:YFL027C; -.
DR   eggNOG; KOG2595; Eukaryota.
DR   GeneTree; ENSGT00390000014944; -.
DR   HOGENOM; CLU_039465_3_0_1; -.
DR   InParanoid; P43570; -.
DR   OMA; YLRDFMM; -.
DR   BioCyc; YEAST:G3O-30433-MON; -.
DR   Reactome; R-SCE-204005; COPII-mediated vesicle transport.
DR   PRO; PR:P43570; -.
DR   Proteomes; UP000002311; Chromosome VI.
DR   RNAct; P43570; protein.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0005096; F:GTPase activator activity; IDA:SGD.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IEA:InterPro.
DR   GO; GO:0016192; P:vesicle-mediated transport; IMP:SGD.
DR   InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR   InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR   InterPro; IPR045913; TBC20/Gyp8-like.
DR   PANTHER; PTHR20913; PTHR20913; 1.
DR   Pfam; PF00566; RabGAP-TBC; 1.
DR   SUPFAM; SSF47923; SSF47923; 1.
DR   PROSITE; PS50086; TBC_RABGAP; 1.
PE   1: Evidence at protein level;
KW   GTPase activation; Reference proteome.
FT   CHAIN           1..497
FT                   /note="GTPase-activating protein GYP8"
FT                   /id="PRO_0000208018"
FT   DOMAIN          69..281
FT                   /note="Rab-GAP TBC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
SQ   SEQUENCE   497 AA;  57619 MW;  C93C57821B50F1E5 CRC64;
     MPLRSLFHTN QSSHDKDALT RGGYNAYLES LSRCDSGKAE EQKGKVISKL LEKKDVRALR
     YIGLGPLGFV NNSLRKDCWY ELLASQLLID DATEYITPVE KHKDEGQVIL DAERSFGGIV
     DKNLKLQLRK LLVELITRVL RKYPTLNYYQ GYHDIVSVFI MCFSWNVTKE NGLELENLSL
     QEEIDMERLF YCIEAFTLLY LRDFMMNSLD FSFEQLRVIS SLIKESNMKF YNLFKFDENE
     PLFAIGSILT IFAHNLKPID SGDNNLHKIL FQIFDMTISM QSMRLPLIIY KNLLLQNASE
     ISKQIEANSD FFENDFDLRH GAIQTVLQKK LYDEALWEEV LQITRKDATT ASKKALKRVS
     LNKYSALLNT ACGKPGCFDM STIIFYLSEQ TKMNEHYKEE KYHGVAARSK TRALVQRLGH
     FLPSKYNRWS KISLLIGIVA ILYQLRTTRS LSLVLNLRYM ISTKLKDLSH ININLHHVSH
     IWVDPIRDIL KLGHPTR