ID   AMY1_CAPAA              Reviewed;          14 AA.
AC   P86072;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 14.
DE   RecName: Full=Alpha-amylase 1 {ECO:0000250|UniProtKB:P04063};
DE            EC=3.2.1.1;
DE   AltName: Full=1,4-alpha-D-glucan glucanohydrolase {ECO:0000250|UniProtKB:P04063};
DE   Flags: Fragment;
OS   Capsicum annuum var. annuum (Red pepper).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Capsiceae; Capsicum.
OX   NCBI_TaxID=40321;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE.
RA   Almagro L., Sabater Jara A.B., Pedreno M.A.;
RL   Submitted (JUL-2008) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in
CC         polysaccharides containing three or more (1->4)-alpha-linked D-
CC         glucose units.; EC=3.2.1.1;
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000250|UniProtKB:P04063};
CC       Note=Binds 3 Ca(2+) ions per subunit. {ECO:0000250|UniProtKB:P04063};
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:P04063}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. {ECO:0000255}.
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DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Calcium; Carbohydrate metabolism; Direct protein sequencing; Glycosidase;
KW   Hydrolase; Metal-binding.
FT   CHAIN           <1..>14
FT                   /note="Alpha-amylase 1"
FT                   /id="PRO_0000362982"
FT   BINDING         7
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P04063"
FT   BINDING         10
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P04063"
FT   BINDING         12
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P04063"
FT   UNSURE          2
FT                   /note="I or L"
FT   UNSURE          5
FT                   /note="I or L"
FT   UNSURE          6
FT                   /note="F or M"
FT   NON_TER         1
FT   NON_TER         14
SQ   SEQUENCE   14 AA;  1543 MW;  638ED57656D2E37B CRC64;
     GIYCIFEGGT PDDR