ID   AMY1_CYTFI              Reviewed;          10 AA.
AC   C0HJE4;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2013, sequence version 1.
DT   25-MAY-2022, entry version 8.
DE   RecName: Full=Alpha-amylase {ECO:0000303|Ref.1};
DE            EC=3.2.1.1 {ECO:0000269|Ref.1};
DE   AltName: Full=1,4-alpha-D-glucan glucanohydrolase {ECO:0000250|UniProtKB:P00691};
DE   Flags: Fragment;
OS   Cytobacillus firmus (Bacillus firmus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Cytobacillus.
OX   NCBI_TaxID=1399;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=KIBGE-IB28 {ECO:0000269|Ref.1};
RA   Zohra R.R., Nawaz A., Bibi Z., Aman A., Qader S.A.;
RT   "Purification and characterization of thermostable alpha-amylase from
RT   Bacillus firmus KIBGE-IB28.";
RL   Submitted (AUG-2013) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in
CC         polysaccharides containing three or more (1->4)-alpha-linked D-
CC         glucose units.; EC=3.2.1.1; Evidence={ECO:0000269|Ref.1};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         Note=Because of the indeterminate molecular weight of starch, an
CC         approximate KM value of 0.975 mg/ml was determined.
CC         {ECO:0000269|Ref.1};
CC       pH dependence:
CC         Optimum pH is 5.0. {ECO:0000269|Ref.1};
CC       Temperature dependence:
CC         Optimum temperature is 60 degrees Celsius. {ECO:0000269|Ref.1};
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is: 5.0,
CC       its MW is: 155 kDa. {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. {ECO:0000305}.
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DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Direct protein sequencing; Glycosidase; Hydrolase;
KW   Polysaccharide degradation.
FT   CHAIN           1..>10
FT                   /note="Alpha-amylase"
FT                   /id="PRO_0000424176"
FT   NON_TER         10
FT                   /evidence="ECO:0000303|Ref.1"
SQ   SEQUENCE   10 AA;  1150 MW;  D9F28FC2C3333862 CRC64;
     MLVGKKVQTF