GYRA_STRR6
ID GYRA_STRR6 Reviewed; 822 AA.
AC Q8DPM2;
DT 16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=DNA gyrase subunit A {ECO:0000255|HAMAP-Rule:MF_01897};
DE EC=5.6.2.2 {ECO:0000255|HAMAP-Rule:MF_01897};
GN Name=gyrA {ECO:0000255|HAMAP-Rule:MF_01897}; OrderedLocusNames=spr1099;
OS Streptococcus pneumoniae (strain ATCC BAA-255 / R6).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=171101;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9603872; DOI=10.1128/jb.180.11.2854-2861.1998;
RA Balas D., Fernandez-Moreira E., de la Campa A.G.;
RT "Molecular characterization of the gene encoding the DNA gyrase A subunit
RT of Streptococcus pneumoniae.";
RL J. Bacteriol. 180:2854-2861(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-255 / R6;
RX PubMed=11544234; DOI=10.1128/jb.183.19.5709-5717.2001;
RA Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J.,
RA Lee L.N., Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M.,
RA McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B.,
RA Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y.,
RA Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R.,
RA Rosteck P.R. Jr., Skatrud P.L., Glass J.I.;
RT "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL J. Bacteriol. 183:5709-5717(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 44-145.
RA Janoir C.;
RL Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: A type II topoisomerase that negatively supercoils closed
CC circular double-stranded (ds) DNA in an ATP-dependent manner to
CC modulate DNA topology and maintain chromosomes in an underwound state.
CC Negative supercoiling favors strand separation, and DNA replication,
CC transcription, recombination and repair, all of which involve strand
CC separation. Also able to catalyze the interconversion of other
CC topological isomers of dsDNA rings, including catenanes and knotted
CC rings. Type II topoisomerases break and join 2 DNA strands
CC simultaneously in an ATP-dependent manner. {ECO:0000255|HAMAP-
CC Rule:MF_01897}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01897};
CC -!- SUBUNIT: Heterotetramer, composed of two GyrA and two GyrB chains. In
CC the heterotetramer, GyrA contains the active site tyrosine that forms a
CC transient covalent intermediate with DNA, while GyrB binds cofactors
CC and catalyzes ATP hydrolysis. {ECO:0000255|HAMAP-Rule:MF_01897}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01897}.
CC -!- MISCELLANEOUS: Few gyrases are as efficient as E.coli at forming
CC negative supercoils. Not all organisms have 2 type II topoisomerases;
CC in organisms with a single type II topoisomerase this enzyme also has
CC to decatenate newly replicated chromosomes. {ECO:0000255|HAMAP-
CC Rule:MF_01897}.
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC family. {ECO:0000255|HAMAP-Rule:MF_01897}.
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DR EMBL; AF053121; AAC23563.1; -; Genomic_DNA.
DR EMBL; AE007317; AAK99902.1; -; Genomic_DNA.
DR EMBL; Y07845; CAA69173.1; -; Genomic_DNA.
DR PIR; B98009; B98009.
DR RefSeq; NP_358692.1; NC_003098.1.
DR RefSeq; WP_001152994.1; NC_003098.1.
DR PDB; 4Z2C; X-ray; 3.19 A; A/B=1-493.
DR PDB; 4Z2D; X-ray; 3.38 A; A/B=1-493.
DR PDB; 4Z2E; X-ray; 3.46 A; A/B=1-493.
DR PDB; 6N1P; EM; 6.35 A; A/B/C/D/E/F/G/H=1-487.
DR PDB; 6N1Q; EM; 5.16 A; A/B/C/D/E/F/G/H=1-487.
DR PDB; 6N1R; EM; 4.00 A; A/B/C/D/E/F/G/H/I/J/K/L=1-487.
DR PDBsum; 4Z2C; -.
DR PDBsum; 4Z2D; -.
DR PDBsum; 4Z2E; -.
DR PDBsum; 6N1P; -.
DR PDBsum; 6N1Q; -.
DR PDBsum; 6N1R; -.
DR AlphaFoldDB; Q8DPM2; -.
DR SMR; Q8DPM2; -.
DR STRING; 171101.spr1099; -.
DR EnsemblBacteria; AAK99902; AAK99902; spr1099.
DR GeneID; 60233881; -.
DR KEGG; spr:spr1099; -.
DR PATRIC; fig|171101.6.peg.1194; -.
DR eggNOG; COG0188; Bacteria.
DR HOGENOM; CLU_002977_6_1_9; -.
DR OMA; THHWLLF; -.
DR Proteomes; UP000000586; Chromosome.
DR GO; GO:0005694; C:chromosome; IEA:InterPro.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0009330; C:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0034335; F:DNA negative supercoiling activity; IEA:UniProt.
DR GO; GO:0006265; P:DNA topological change; IBA:GO_Central.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd00187; TOP4c; 1.
DR Gene3D; 1.10.268.10; -; 1.
DR Gene3D; 2.120.10.90; -; 1.
DR Gene3D; 3.90.199.10; -; 1.
DR HAMAP; MF_01897; GyrA; 1.
DR InterPro; IPR005743; GyrA.
DR InterPro; IPR006691; GyrA/parC_rep.
DR InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR013758; Topo_IIA_A/C_ab.
DR InterPro; IPR013757; Topo_IIA_A_a_sf.
DR InterPro; IPR002205; Topo_IIA_dom_A.
DR Pfam; PF03989; DNA_gyraseA_C; 6.
DR Pfam; PF00521; DNA_topoisoIV; 1.
DR SMART; SM00434; TOP4c; 1.
DR SUPFAM; SSF101904; SSF101904; 1.
DR SUPFAM; SSF56719; SSF56719; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cytoplasm; DNA-binding; Isomerase;
KW Nucleotide-binding; Reference proteome; Topoisomerase.
FT CHAIN 1..822
FT /note="DNA gyrase subunit A"
FT /id="PRO_0000145263"
FT MOTIF 524..530
FT /note="GyrA-box"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01897"
FT ACT_SITE 120
FT /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01897"
FT CONFLICT 537
FT /note="K -> E (in Ref. 1; AAC23563)"
FT /evidence="ECO:0000305"
FT CONFLICT 618..620
FT /note="GIV -> VLL (in Ref. 1; AAC23563)"
FT /evidence="ECO:0000305"
FT STRAND 6..10
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 11..29
FT /evidence="ECO:0007829|PDB:4Z2C"
FT TURN 35..38
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 41..53
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 57..59
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 64..74
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 80..89
FT /evidence="ECO:0007829|PDB:4Z2C"
FT TURN 93..95
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 100..105
FT /evidence="ECO:0007829|PDB:4Z2C"
FT TURN 118..120
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 122..125
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 129..133
FT /evidence="ECO:0007829|PDB:4Z2C"
FT TURN 134..139
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 143..145
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 149..156
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 163..167
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 169..172
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 177..180
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 185..197
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 203..207
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 220..222
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 225..233
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 234..241
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 243..248
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 250..252
FT /evidence="ECO:0007829|PDB:4Z2E"
FT STRAND 254..260
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 267..278
FT /evidence="ECO:0007829|PDB:4Z2C"
FT TURN 279..281
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 286..291
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 300..304
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 310..320
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 324..330
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 332..335
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 338..341
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 344..385
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 387..395
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 400..411
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 415..422
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 426..429
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 433..456
FT /evidence="ECO:0007829|PDB:4Z2C"
FT HELIX 458..476
FT /evidence="ECO:0007829|PDB:4Z2C"
FT STRAND 482..485
FT /evidence="ECO:0007829|PDB:4Z2C"
SQ SEQUENCE 822 AA; 92039 MW; 130307B8B5160EAD CRC64;
MQDKNLVNVN LTKEMKASFI DYAMSVIVAR ALPDVRDGLK PVHRRILYGM NELGVTPDKP
HKKSARITGD VMGKYHPHGD SSIYEAMVRM AQWWSYRYML VDGHGNFGSM DGDSAAAQRY
TEARMSKIAL EMLRDINKNT VDFVDNYDAN EREPLVLPAR FPNLLVNGAT GIAVGMATNI
PPHNLGETID AVKLVMDNPE VTTKDLMEVL PGPDFPTGAL VMGKSGIHKA YETGKGSIVL
RSRTEIETTK TGRERIVVTE FPYMVNKTKV HEHIVRLVQE KRIEGITAVR DESNREGVRF
VIEVKRDASA NVILNNLFKM TQMQTNFGFN MLAIQNGIPK ILSLRQILDA YIEHQKEVVV
RRTRFDKEKA EARAHILEGL LIALDHIDEV IRIIRASETD AEAQAELMSK FKLSERQSQA
ILDMRLRRLT GLERDKIQSE YDDLLALIAD LADILAKPER VSQIIKDELD EVKRKFSDKR
RTELMVGQVL SLEDEDLIEE SDVLITLSNR GYIKRLDQDE FTAQKRGGRG VQGTGVKDDD
FVRELVSTST HDHLLFFTNK GRVYRLKGYE IPEYGRTAKG LPVVNLLKLD EDESIQTVIN
VESDRSDDAY LFFTTRHGIV KRTSVKEFAN IRQNGLKALN LKDEDELINV LLAEGDMDII
IGTKFGYAVR FNQSAVRGMS RIATGVKGVN LREGDTVVGA SLITDQDEVL IITEKGYGKR
TVATEYPTKG RGGKGMQTAK ITEKNGLLAG LMTVQGDEDL MIITDTGVMI RTNLANISQT
GRATMGVKVM RLDQDAQIVT FTTVAVAEKE EVGTENETEG EA
