GYS_DICDI
ID GYS_DICDI Reviewed; 878 AA.
AC Q55GH4;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Glycogen [starch] synthase;
DE EC=2.4.1.11 {ECO:0000269|PubMed:9023987};
GN Name=glcS; ORFNames=DDB_G0267674;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP PRELIMINARY NUCLEOTIDE SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND
RP BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=9023987;
RX DOI=10.1002/(sici)1520-6408(1996)19:4<350::aid-dvg8>3.0.co;2-8;
RA Williamson B.D., Favis R., Brickey D.A., Rutherford C.L.;
RT "Isolation and characterization of glycogen synthase in Dictyostelium
RT discoideum.";
RL Dev. Genet. 19:350-364(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Catalyzes the formation of apha-1,4 glycosidic bonds adding
CC glucose residue from UDPG to the growing chain of glycogen.
CC {ECO:0000269|PubMed:9023987}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->4)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1->4)-
CC alpha-D-glucosyl](n+1) + H(+) + UDP; Xref=Rhea:RHEA:18549, Rhea:RHEA-
CC COMP:9584, Rhea:RHEA-COMP:9587, ChEBI:CHEBI:15378, ChEBI:CHEBI:15444,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885; EC=2.4.1.11;
CC Evidence={ECO:0000269|PubMed:9023987};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18550;
CC Evidence={ECO:0000269|PubMed:9023987};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is between 7.5 and 8.0. {ECO:0000269|PubMed:9023987};
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC {ECO:0000305|PubMed:9023987}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 3 family. {ECO:0000305}.
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DR EMBL; AAFI02000003; EAL73290.1; -; Genomic_DNA.
DR RefSeq; XP_647210.1; XM_642118.1.
DR AlphaFoldDB; Q55GH4; -.
DR SMR; Q55GH4; -.
DR STRING; 44689.DDB0231962; -.
DR PaxDb; Q55GH4; -.
DR EnsemblProtists; EAL73290; EAL73290; DDB_G0267674.
DR GeneID; 8616014; -.
DR KEGG; ddi:DDB_G0267674; -.
DR dictyBase; DDB_G0267674; glcS.
DR eggNOG; KOG3742; Eukaryota.
DR HOGENOM; CLU_015910_1_0_1; -.
DR InParanoid; Q55GH4; -.
DR OMA; WEACGVG; -.
DR PhylomeDB; Q55GH4; -.
DR Reactome; R-DDI-3322077; Glycogen synthesis.
DR UniPathway; UPA00164; -.
DR PRO; PR:Q55GH4; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004373; F:glycogen (starch) synthase activity; IDA:dictyBase.
DR GO; GO:0000045; P:autophagosome assembly; IMP:dictyBase.
DR GO; GO:0005978; P:glycogen biosynthetic process; IDA:dictyBase.
DR GO; GO:0031288; P:sorocarp morphogenesis; IMP:dictyBase.
DR InterPro; IPR008631; Glycogen_synth.
DR PANTHER; PTHR10176; PTHR10176; 1.
DR Pfam; PF05693; Glycogen_syn; 1.
PE 1: Evidence at protein level;
KW Glycogen biosynthesis; Glycosyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..878
FT /note="Glycogen [starch] synthase"
FT /id="PRO_0000355639"
FT REGION 637..721
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 746..878
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 646..721
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 61
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
SQ SEQUENCE 878 AA; 97515 MW; CA7F313E52021AB3 CRC64;
MIFGTPNSTF LENRLGSSTG MPFSSATSLN SGVDQMMNEL NIQQPTSHTV LFDLSWEVAK
KVGGIYTVLK TKAPVTVEEY KSRYALIGPY NASTAPTEFE PLIPGPLSSP IIENMMKKYG
IHVHFGKWLV EGYPKVFLID LHSSMHKLGE WRWDLMSGFE QAGDNETNET IVFGYQSALL
LKEFAEANPN DKYIAHFHEW QASVGLILLK KWKVPVSTIF TTHATLLGRY LAAGGVDLYN
QMQVLNMDFE ASKRGIYHRH WIEKKSAADS HVFTTVSEIT GYESEHILMR KPDVILPNGL
KLDKFTALHE FQNLHAKYKG VLNEFVRGHF YGHYSDFDLD NTLYVFTAGR HEYFNKGVDM
FLDSLAGLNK LLQQSGSKMT VVAFIIMPAA TNNFNVESLK GHSYLKDMRR TCNTIVEAMG
ERLFEATSRG KMISPEELLS QEDLVMLKRR IFALKQKSSG PPVVTHNMIN DNDEILQHIR
RIKLFNSQED RVKVIYHPEF LTSTNPLIPL DYTEFVRGCH LGIFPSYYEP FGMTPAECCA
SGCPSITSNL TGFANYMSRA LQDTDSKGIF IVDRRFKSSR ETVDQMTQYL WKFTQLDRRQ
RIELRNATEK LSELLDWRTL GKFYKTARAL ALERAFPPKP ISRSPSPSPS SSLKLSTGLS
NQIELQQQQQ QQQPQPIGTT INLIPPSSNV SVTPTTTPTT TTTATTATTA PITTPKPNVI
PINTGKENIT LLSPNSMSSL LSDSLNEFKK QQQQQQQSKT PTTPTTTSTT TTTPSTTAAA
TNKSVLSNPT PTPSPNTSSF IPTNKGSTAT TTTTTATPTP TPSNNTNGKP FNPIEALTKS
NSSSNFATTS TASVNTNNGG TNSPVSKSIP IPSSKSLK
