GZF1_MOUSE
ID GZF1_MOUSE Reviewed; 706 AA.
AC Q4VBD9; Q8BGY0;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=GDNF-inducible zinc finger protein 1;
DE AltName: Full=Zinc finger protein 336;
GN Name=Gzf1 {ECO:0000312|MGI:MGI:1921783}; Synonyms=Zfp336;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=14522971; DOI=10.1074/jbc.m309629200;
RA Fukuda N., Ichihara M., Morinaga T., Kawai K., Hayashi H., Murakumo Y.,
RA Matsuo S., Takahashi M.;
RT "Identification of a novel glial cell line-derived neurotrophic factor-
RT inducible gene required for renal branching morphogenesis.";
RL J. Biol. Chem. 278:50386-50392(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, Eye, and Mammary gland;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryonic brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-611, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Pancreas, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=28475863; DOI=10.1016/j.ajhg.2017.04.008;
RA Patel N., Shamseldin H.E., Sakati N., Khan A.O., Softa A., Al-Fadhli F.M.,
RA Hashem M., Abdulwahab F.M., Alshidi T., Alomar R., Alobeid E., Wakil S.M.,
RA Colak D., Alkuraya F.S.;
RT "GZF1 Mutations Expand the Genetic Heterogeneity of Larsen Syndrome.";
RL Am. J. Hum. Genet. 100:831-836(2017).
CC -!- FUNCTION: Transcriptional repressor that binds the GZF1 responsive
CC element (GRE) (consensus: 5'-TGCGCN[TG][CA]TATA-3'). May be regulating
CC VSX2/HOX10 expression. {ECO:0000250|UniProtKB:Q9H116}.
CC -!- SUBUNIT: Interacts with NCL. {ECO:0000250|UniProtKB:Q9H116}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14522971,
CC ECO:0000269|PubMed:28475863}. Cytoplasm {ECO:0000269|PubMed:14522971,
CC ECO:0000269|PubMed:28475863}. Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q9H116}. Note=Predominantly nuclear.
CC {ECO:0000269|PubMed:14522971}.
CC -!- TISSUE SPECIFICITY: Expressed in several tissues, with highest levels
CC in liver. Also expressed in embryos from 7 to 17 dpc.
CC {ECO:0000269|PubMed:14522971}.
CC -!- DEVELOPMENTAL STAGE: At 13.5-14.5 dpc, strong expression in ureteric
CC buds (at protein level). Expression decreases in the kidney after birth
CC (at protein level). Expressed in the eyes and limbs during development
CC (at protein level). {ECO:0000269|PubMed:14522971,
CC ECO:0000269|PubMed:28475863}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AB100266; BAC98465.1; -; mRNA.
DR EMBL; AK052820; BAC35160.1; -; mRNA.
DR EMBL; AK053262; BAC35327.1; -; mRNA.
DR EMBL; AK141153; BAE24571.1; -; mRNA.
DR EMBL; AL928638; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466519; EDL28537.1; -; Genomic_DNA.
DR EMBL; CH466519; EDL28538.1; -; Genomic_DNA.
DR EMBL; BC096015; AAH96015.1; -; mRNA.
DR CCDS; CCDS16841.1; -.
DR RefSeq; NP_083262.1; NM_028986.3.
DR RefSeq; XP_006500367.1; XM_006500304.3.
DR RefSeq; XP_006500368.1; XM_006500305.3.
DR AlphaFoldDB; Q4VBD9; -.
DR SMR; Q4VBD9; -.
DR BioGRID; 216825; 31.
DR STRING; 10090.ENSMUSP00000028928; -.
DR iPTMnet; Q4VBD9; -.
DR PhosphoSitePlus; Q4VBD9; -.
DR EPD; Q4VBD9; -.
DR jPOST; Q4VBD9; -.
DR MaxQB; Q4VBD9; -.
DR PaxDb; Q4VBD9; -.
DR PeptideAtlas; Q4VBD9; -.
DR PRIDE; Q4VBD9; -.
DR ProteomicsDB; 271354; -.
DR Antibodypedia; 9733; 59 antibodies from 20 providers.
DR DNASU; 74533; -.
DR Ensembl; ENSMUST00000028928; ENSMUSP00000028928; ENSMUSG00000027439.
DR GeneID; 74533; -.
DR KEGG; mmu:74533; -.
DR UCSC; uc008mtg.2; mouse.
DR CTD; 64412; -.
DR MGI; MGI:1921783; Gzf1.
DR VEuPathDB; HostDB:ENSMUSG00000027439; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00870000136554; -.
DR HOGENOM; CLU_018348_1_0_1; -.
DR InParanoid; Q4VBD9; -.
DR OMA; VVYRCET; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q4VBD9; -.
DR TreeFam; TF350965; -.
DR BioGRID-ORCS; 74533; 6 hits in 73 CRISPR screens.
DR ChiTaRS; Gzf1; mouse.
DR PRO; PR:Q4VBD9; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q4VBD9; protein.
DR Bgee; ENSMUSG00000027439; Expressed in animal zygote and 227 other tissues.
DR ExpressionAtlas; Q4VBD9; baseline and differential.
DR Genevisible; Q4VBD9; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0001658; P:branching involved in ureteric bud morphogenesis; IMP:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF00096; zf-C2H2; 6.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00355; ZnF_C2H2; 10.
DR SUPFAM; SSF54695; SSF54695; 1.
DR SUPFAM; SSF57667; SSF57667; 6.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 10.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 10.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..706
FT /note="GDNF-inducible zinc finger protein 1"
FT /id="PRO_0000409660"
FT DOMAIN 31..103
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT ZN_FING 315..337
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 346..369
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 375..398
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 405..427
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 433..455
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 461..483
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 489..511
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 517..539
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 545..567
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 573..595
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 149..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 242..308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 149..169
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 197..216
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..276
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 291..308
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 611
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 187
FT /note="R -> T (in Ref. 5; AAH96015)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 706 AA; 79533 MW; 63E082642FC0956C CRC64;
MESGTVLLES KSSPLNLLHE MHELRLLGHL CDVTVSIENQ GVHEDFMAHK AVLAATSKFF
KEVFLNEKSA DGTRTNVYLN EVQAVDFASF LEFVYTAKVR VEEDRVQQML EVAEKLKCLD
LSETCLQLKK QMLESVLLEL QNFSESQEVE ASSGPQVSVT PSSKASVPGE DAHSNGLVDS
SDYPIERLGN GLSPETPSKK CKEKLDKKKD VAKPPFPKIR RASGRLAGKK VFVEIPKKKY
TRRLREQQKS AEEAAENDKC PQDQSPDNER METEPAAKSE ACPASVELEE SLQKVEGEKE
EEEGKDGEEK KKSNFQCTVC DKAFLYEKSF LKHIKYHHGV ATEVVYRCDT CGQTFANRCN
LKSHQRHVHS SERHFPCEMC AKKFKRKKDV KRHVLQVHEG GGERHRCGQC GKGLSSKTAL
RLHERTHTGD KPYGCTKCDA KFSQPSALKT HLRVHTGERP FVCDECGARF TQNHMLIYHK
RCHTGERPFM CETCGKSFAS KEYLKHHNRI HTGSKPFKCE VCLRTFAQRN SLYQHIKVHT
GERPYCCDQC GKQFTQVNAL QRHHRIHTGE KPYMCNACGR TFTDKSTLRR HTSIHDKNTP
WKSFLVIVDG SPKNDEGHKT EQPDDEYASP KLSDRLLSFG ENSHFNNLLE VQGNVPAVQE
NSSTGAACKA VVSQDALLTT SISALGELTP QAVSMPAHLP SLTNME
