G_BPG4
ID G_BPG4 Reviewed; 177 AA.
AC P03644;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 23-FEB-2022, entry version 126.
DE RecName: Full=Major spike protein G;
DE AltName: Full=G protein;
DE AltName: Full=GPG;
GN Name=G;
OS Escherichia phage G4 (Bacteriophage G4).
OC Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes;
OC Petitvirales; Microviridae; Bullavirinae; Gequatrovirus.
OX NCBI_TaxID=10843;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=714153; DOI=10.1038/276236a0;
RA Godson G.N., Barrell B.G., Staden R., Fiddes J.C.;
RT "Nucleotide sequence of bacteriophage G4 DNA.";
RL Nature 276:236-247(1978).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-81.
RX PubMed=277911; DOI=10.1073/pnas.75.7.3094;
RA Sims J., Dressler D.;
RT "Site-specific initiation of a DNA fragment: nucleotide sequence of the
RT bacteriophage G4 negative-strand initiation site.";
RL Proc. Natl. Acad. Sci. U.S.A. 75:3094-3098(1978).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
RX PubMed=8642594; DOI=10.1006/jmbi.1996.0121;
RA McKenna R., Bowman B.R., Iiag L.L., Rossmann M.G., Fane B.A.;
RT "Atomic structure of the degraded procapsid particle of the bacteriophage
RT G4: induced structural changes in the presence of calcium ions and
RT functional implications.";
RL J. Mol. Biol. 256:736-750(1996).
CC -!- FUNCTION: Attaches the circulating virion to the bacterial
CC lipopolysaccharides which serve as receptor for the virus. Determines
CC the phage host-range. Probably triggers with protein H the injection of
CC the phage DNA into the host cytoplasm upon conformational changes
CC induced by the interaction with host lipopolysaccharides (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: The virion is composed of 60 copies each of the F, G, and J
CC proteins, and 12 copies of the H protein. There are 12 spikes which are
CC each composed of 5 G and one H proteins.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the microvirus G protein family. {ECO:0000305}.
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DR EMBL; V00657; CAA24020.1; -; Genomic_DNA.
DR EMBL; M25080; AAA32328.1; -; Genomic_DNA.
DR PIR; A04252; ZGBPG4.
DR PDB; 1GFF; X-ray; 3.00 A; 2=1-177.
DR PDBsum; 1GFF; -.
DR SMR; P03644; -.
DR DIP; DIP-6178N; -.
DR MINT; P03644; -.
DR EvolutionaryTrace; P03644; -.
DR Proteomes; UP000002140; Genome.
DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0019048; P:modulation by virus of host process; IEA:InterPro.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.20; -; 1.
DR InterPro; IPR016184; Capsid/spike_ssDNA_virus.
DR InterPro; IPR003515; Spike_G.
DR InterPro; IPR029053; Viral_coat.
DR Pfam; PF02306; Phage_G; 1.
DR PIRSF; PIRSF004159; Spike_G; 1.
DR SUPFAM; SSF88645; SSF88645; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Capsid protein; Host-virus interaction; Reference proteome;
KW Viral attachment to host cell;
KW Viral genome ejection through host cell envelope;
KW Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT CHAIN 1..177
FT /note="Major spike protein G"
FT /id="PRO_0000164893"
FT CONFLICT 27
FT /note="A -> T (in Ref. 2; AAA32328)"
FT /evidence="ECO:0000305"
FT STRAND 19..22
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 29..32
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 36..41
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 44..47
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 50..53
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 55..61
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 69..81
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 87..96
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 109..111
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 115..117
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 122..124
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 126..131
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 139..148
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 150..152
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 154..157
FT /evidence="ECO:0007829|PDB:1GFF"
FT STRAND 159..168
FT /evidence="ECO:0007829|PDB:1GFF"
SQ SEQUENCE 177 AA; 18820 MW; 40ED2E63CA9EC384 CRC64;
MFQKFISKHN APINSTQLAA TKTPAVAAPV LSVPNLSRST ILINATTTAV TTHSGLCHVV
RIDETNPTNH HALSIAGSLS NVPADMIAFA IRFEVADGVV PTAVPALYDV YPIETFNNGK
AISFKDAVTI DSHPRTVGND VYAGIMLWSN AWTASTISGV LSVNQVNREA TVLQPLK
