ID   G_LAMBD                 Reviewed;         140 AA.
AC   P03734;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   02-JUN-2021, entry version 80.
DE   RecName: Full=Tail assembly protein G {ECO:0000255|HAMAP-Rule:MF_04134};
DE   AltName: Full=Gene product G {ECO:0000255|HAMAP-Rule:MF_04134};
DE            Short=gpG {ECO:0000255|HAMAP-Rule:MF_04134};
DE   AltName: Full=Minor tail protein G {ECO:0000255|HAMAP-Rule:MF_04134};
DE   AltName: Full=Tail assembly chaperone {ECO:0000255|HAMAP-Rule:MF_04134};
DE            Short=TAC {ECO:0000255|HAMAP-Rule:MF_04134};
GN   Name=G; OrderedLocusNames=lambdap14;
OS   Escherichia phage lambda (Bacteriophage lambda).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Lambdavirus.
OX   NCBI_TaxID=10710;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=6221115; DOI=10.1016/0022-2836(82)90546-0;
RA   Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B.;
RT   "Nucleotide sequence of bacteriophage lambda DNA.";
RL   J. Mol. Biol. 162:729-773(1982).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 117-140.
RX   PubMed=8230192; DOI=10.1006/jmbi.1993.1568;
RA   Levin M.E., Hendrix R.W., Casjens S.R.;
RT   "A programmed translational frameshift is required for the synthesis of a
RT   bacteriophage lambda tail assembly protein.";
RL   J. Mol. Biol. 234:124-139(1993).
RN   [3]
RP   FUNCTION.
RX   PubMed=23851014; DOI=10.1016/j.jmb.2013.07.002;
RA   Xu J., Hendrix R.W., Duda R.L.;
RT   "A balanced ratio of proteins from gene G and frameshift-extended gene GT
RT   is required for phage lambda tail assembly.";
RL   J. Mol. Biol. 425:3476-3487(2013).
RN   [4]
RP   FUNCTION, INTERACTION WITH THE TAIL ASSEMBLY PROTEIN GT, AND INTERACTION
RP   WITH TAPE MEASURE PROTEIN.
RX   PubMed=23911548; DOI=10.1016/j.jmb.2013.06.040;
RA   Xu J., Hendrix R.W., Duda R.L.;
RT   "Chaperone-protein interactions that mediate assembly of the bacteriophage
RT   lambda tail to the correct length.";
RL   J. Mol. Biol. 426:1004-1018(2014).
RN   [5]
RP   REVIEW.
RX   PubMed=15469818; DOI=10.1016/j.molcel.2004.09.006;
RA   Xu J., Hendrix R.W., Duda R.L.;
RT   "Conserved translational frameshift in dsDNA bacteriophage tail assembly
RT   genes.";
RL   Mol. Cell 16:11-21(2004).
CC   -!- FUNCTION: Promotes tail assembly by creating a scaffold for the tail
CC       tube proteins. Tail assembly proteins G and GT probably wrap the linear
CC       tape measure protein to create a tail assembly scaffold. This allows
CC       the polymerization of the tail tube protein, during which G and GT are
CC       released, therefore they are absent in the mature virion. The tail
CC       assembly protein GT is produced by a rare -1 ribosomal frameshift. The
CC       ratio of translated G/GT is about 20, and this ratio is important for
CC       proper tail assembly. {ECO:0000255|HAMAP-Rule:MF_04134,
CC       ECO:0000269|PubMed:23851014, ECO:0000269|PubMed:23911548}.
CC   -!- SUBUNIT: Interacts with the tail assembly protein GT and the tape
CC       measure protein. {ECO:0000255|HAMAP-Rule:MF_04134,
CC       ECO:0000269|PubMed:23911548}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04134}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Ribosomal frameshifting; Named isoforms=2;
CC         Comment=The tail assembly protein GT is produced by a rare -1
CC         ribosomal frameshift. This expression strategy assures a fixed ration
CC         of G/GT.;
CC       Name=Tail assembly protein G;
CC         IsoId=P03734-1; Sequence=Displayed;
CC       Name=Tail assembly protein GT;
CC         IsoId=P03735-1; Sequence=External;
CC   -!- MISCELLANEOUS: [Isoform Tail assembly protein G]: Main product of the
CC       GT gene, representing 96% of the translated protein.
CC   -!- SIMILARITY: Belongs to the lambda-like tail assembly protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_04134}.
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DR   EMBL; J02459; AAA96546.1; -; Genomic_DNA.
DR   EMBL; S66722; AAB28723.1; -; Genomic_DNA.
DR   PIR; C43014; TLBPTL.
DR   PIR; E43008; TLBPGL.
DR   RefSeq; NP_040593.1; NC_001416.1. [P03734-1]
DR   IntAct; P03734; 13.
DR   GeneID; 2703488; -.
DR   KEGG; vg:2703488; -.
DR   Proteomes; UP000001711; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0098003; P:viral tail assembly; IDA:UniProtKB.
DR   HAMAP; MF_04134; GT_LAMBD; 1.
DR   InterPro; IPR010027; Tail_assembly_G.
DR   InterPro; IPR043704; Tail_assembly_GT.
DR   Pfam; PF06894; Phage_TAC_2; 1.
DR   TIGRFAMs; TIGR01674; phage_lambda_G; 1.
PE   1: Evidence at protein level;
KW   Host cytoplasm; Late protein; Reference proteome; Ribosomal frameshifting;
KW   Viral release from host cell; Viral tail assembly.
FT   CHAIN           1..140
FT                   /note="Tail assembly protein G"
FT                   /id="PRO_0000077665"
FT   REGION          121..140
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   140 AA;  15608 MW;  C50DA2AFD77D2313 CRC64;
     MFLKTESFEH NGVTVTLSEL SALQRIEHLA LMKRQAEQAE SDSNRKFTVE DAIRTGAFLV
     AMSLWHNHPQ KTQMPSMNEA VKQIEQEVLT TWPTEAISHA ENVVYRLSGM YEFVVNNAPE
     QTEDAGPAEP VSAGKCSTVS