ID   H10C1_CYRHA             Reviewed;          65 AA.
AC   D2Y2R4;
DT   02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   02-MAR-2010, sequence version 1.
DT   25-MAY-2022, entry version 23.
DE   RecName: Full=Hainantoxin-X-3;
DE            Short=HNTX-X-3;
DE   Flags: Precursor;
OS   Cyriopagopus hainanus (Chinese bird spider) (Haplopelma hainanum).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Haplopelma.
OX   NCBI_TaxID=209901;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   TISSUE=Venom gland;
RX   PubMed=20192277; DOI=10.1021/pr1000016;
RA   Tang X., Zhang Y., Hu W., Xu D., Tao H., Yang X., Li Y., Jiang L.,
RA   Liang S.;
RT   "Molecular diversification of peptide toxins from the tarantula Haplopelma
RT   hainanum (Ornithoctonus hainana) venom based on transcriptomic, peptidomic,
RT   and genomic analyses.";
RL   J. Proteome Res. 9:2550-2564(2010).
CC   -!- FUNCTION: Reversibly blocks N-type calcium channels (Cav2.2/CACNA1B) in
CC       rat dorsal root ganglion cells. Elicits no toxic symptoms in either
CC       vertebrates or invertebrates during a period of 48 hours post-
CC       injection, when it was assayed in vivo by direct injection into mice
CC       and cockroaches (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 36 family. 02 subfamily.
CC   -!- CAUTION: While it is structurally defined as a knottin it lacks the
CC       conserved Cys residue in position 39. {ECO:0000305}.
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DR   EMBL; GU293141; ADB56957.1; -; Genomic_DNA.
DR   AlphaFoldDB; D2Y2R4; -.
DR   SMR; D2Y2R4; -.
DR   ArachnoServer; AS001672; omega-theraphotoxin-Hhn2c.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Calcium channel impairing toxin; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Signal; Toxin;
KW   Voltage-gated calcium channel impairing toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..37
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000400976"
FT   PEPTIDE         38..65
FT                   /note="Hainantoxin-X-3"
FT                   /id="PRO_0000400977"
FT   DISULFID        46..59
FT                   /evidence="ECO:0000250"
FT   DISULFID        55..64
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   65 AA;  7264 MW;  05AC7C0104DD1918 CRC64;
     MNMKILVLVA VLCLVVSTHA ERHSKTDMED SPMIQERKYL PPGKPCYEAT QKIPCCGVCS
     HNNCT