H17B6_BOVIN
ID H17B6_BOVIN Reviewed; 317 AA.
AC Q3T001;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=17-beta-hydroxysteroid dehydrogenase type 6;
DE Short=17-beta-HSD 6;
DE Short=17-beta-HSD6;
DE EC=1.1.1.105 {ECO:0000250|UniProtKB:Q9R092};
DE EC=1.1.1.209 {ECO:0000250|UniProtKB:Q9R092};
DE EC=1.1.1.239 {ECO:0000250|UniProtKB:Q9R092};
DE EC=1.1.1.53 {ECO:0000250|UniProtKB:Q9R092};
DE EC=1.1.1.62 {ECO:0000250|UniProtKB:Q9R092};
DE AltName: Full=3-alpha->beta-hydroxysteroid epimerase;
DE Short=3-alpha->beta-HSE;
DE AltName: Full=Oxidative 3-alpha hydroxysteroid dehydrogenase;
DE Flags: Precursor;
GN Name=HSD17B6;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Testis;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: NAD-dependent oxidoreductase with broad substrate specificity
CC that shows both oxidative and reductive activity (in vitro). Has 17-
CC beta-hydroxysteroid dehydrogenase activity towards various steroids (in
CC vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone
CC and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid
CC dehydrogenase activity towards androsterone (in vitro). Has retinol
CC dehydrogenase activity towards all-trans-retinol (in vitro). Can
CC convert androsterone to epi-androsterone. Androsterone is first
CC oxidized to 5-alpha-androstane-3,17-dione and then reduced to epi-
CC andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids (By
CC similarity). {ECO:0000250|UniProtKB:Q9R092}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-retinol--[retinol-binding protein] + NAD(+) = all-
CC trans-retinal--[retinol-binding protein] + H(+) + NADH;
CC Xref=Rhea:RHEA:48488, Rhea:RHEA-COMP:14428, Rhea:RHEA-COMP:14430,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17336, ChEBI:CHEBI:17898,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:83228;
CC EC=1.1.1.105; Evidence={ECO:0000250|UniProtKB:Q9R092};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-retinol + NAD(+) = all-trans-retinal + H(+) + NADH;
CC Xref=Rhea:RHEA:21284, ChEBI:CHEBI:15378, ChEBI:CHEBI:17336,
CC ChEBI:CHEBI:17898, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC EC=1.1.1.105; Evidence={ECO:0000250|UniProtKB:Q9R092};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:21285;
CC Evidence={ECO:0000250|UniProtKB:Q9R092};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3alpha-hydroxy-5alpha-androstan-17-one + NAD(+) = 5alpha-
CC androstan-3,17-dione + H(+) + NADH; Xref=Rhea:RHEA:20381,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15994, ChEBI:CHEBI:16032,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.209;
CC Evidence={ECO:0000250|UniProtKB:Q9R092};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20382;
CC Evidence={ECO:0000250|UniProtKB:Q9R092};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:20383;
CC Evidence={ECO:0000250|UniProtKB:Q9R092};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + testosterone = androst-4-ene-3,17-dione + H(+) +
CC NADH; Xref=Rhea:RHEA:14929, ChEBI:CHEBI:15378, ChEBI:CHEBI:16422,
CC ChEBI:CHEBI:17347, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC EC=1.1.1.239; Evidence={ECO:0000250|UniProtKB:Q9R092};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5alpha-androstane-3alpha,17beta-diol + NAD(+) = 17beta-
CC hydroxy-5alpha-androstan-3-one + H(+) + NADH; Xref=Rhea:RHEA:42004,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16330, ChEBI:CHEBI:36713,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.53;
CC Evidence={ECO:0000250|UniProtKB:Q9R092};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:42006;
CC Evidence={ECO:0000250|UniProtKB:Q9R092};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=17beta-estradiol + NAD(+) = estrone + H(+) + NADH;
CC Xref=Rhea:RHEA:24612, ChEBI:CHEBI:15378, ChEBI:CHEBI:16469,
CC ChEBI:CHEBI:17263, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.62;
CC Evidence={ECO:0000250|UniProtKB:Q9R092};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=17beta-estradiol + NADP(+) = estrone + H(+) + NADPH;
CC Xref=Rhea:RHEA:24616, ChEBI:CHEBI:15378, ChEBI:CHEBI:16469,
CC ChEBI:CHEBI:17263, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.62;
CC Evidence={ECO:0000250|UniProtKB:Q9R092};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3alpha-hydroxy-5alpha-pregnan-20-one + NAD(+) = 5alpha-
CC pregnane-3,20-dione + H(+) + NADH; Xref=Rhea:RHEA:41980,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:28952, ChEBI:CHEBI:50169,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC Evidence={ECO:0000250|UniProtKB:O14756};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41981;
CC Evidence={ECO:0000250|UniProtKB:O14756};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5alpha-androstane-3beta,17beta-diol + NAD(+) = 17beta-hydroxy-
CC 5alpha-androstan-3-one + H(+) + NADH; Xref=Rhea:RHEA:42184,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16330, ChEBI:CHEBI:18329,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC Evidence={ECO:0000250|UniProtKB:O14756};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:42186;
CC Evidence={ECO:0000250|UniProtKB:O14756};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3beta-hydroxy-5alpha-androstan-17-one + NAD(+) = 5alpha-
CC androstan-3,17-dione + H(+) + NADH; Xref=Rhea:RHEA:42188,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15994, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:541975;
CC Evidence={ECO:0000250|UniProtKB:O14756};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:42190;
CC Evidence={ECO:0000250|UniProtKB:O14756};
CC -!- SUBCELLULAR LOCATION: Microsome membrane
CC {ECO:0000250|UniProtKB:O14756}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:O14756}; Lumenal side
CC {ECO:0000250|UniProtKB:O14756}. Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:O14756}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:O14756}; Lumenal side
CC {ECO:0000250|UniProtKB:O14756}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BC102626; AAI02627.1; -; mRNA.
DR RefSeq; NP_001030472.1; NM_001035395.2.
DR AlphaFoldDB; Q3T001; -.
DR SMR; Q3T001; -.
DR STRING; 9913.ENSBTAP00000015564; -.
DR PaxDb; Q3T001; -.
DR PeptideAtlas; Q3T001; -.
DR GeneID; 533086; -.
DR KEGG; bta:533086; -.
DR CTD; 8630; -.
DR eggNOG; KOG1610; Eukaryota.
DR InParanoid; Q3T001; -.
DR OrthoDB; 1390068at2759; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044594; F:17-beta-hydroxysteroid dehydrogenase (NAD+) activity; IEA:RHEA.
DR GO; GO:0047024; F:5alpha-androstane-3beta,17beta-diol dehydrogenase activity; ISS:UniProtKB.
DR GO; GO:0047044; F:androstan-3-alpha,17-beta-diol dehydrogenase activity; ISS:UniProtKB.
DR GO; GO:0047023; F:androsterone dehydrogenase activity; ISS:UniProtKB.
DR GO; GO:0004303; F:estradiol 17-beta-dehydrogenase activity; ISS:UniProtKB.
DR GO; GO:0004745; F:NAD-retinol dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0047045; F:testosterone 17-beta-dehydrogenase (NADP+) activity; ISS:UniProtKB.
DR GO; GO:0047035; F:testosterone dehydrogenase (NAD+) activity; ISS:UniProtKB.
DR GO; GO:0062175; P:brexanolone catabolic process; ISS:UniProtKB.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycoprotein; Lipid metabolism; Membrane; Microsome;
KW NAD; Oxidoreductase; Reference proteome; Signal; Steroid metabolism.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..317
FT /note="17-beta-hydroxysteroid dehydrogenase type 6"
FT /id="PRO_0000303210"
FT ACT_SITE 176
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 33..57
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 164
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT CARBOHYD 71
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 161
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 317 AA; 36198 MW; 4B1A2ED7906FBB12 CRC64;
MWLYLAVLLG LYYLLRWFRE RQVVSHLQDK FVFITGCDSG FGNQLARQLD LRGLRVLAGC
LTEQGAEQLR NQTSDRLQTV ILDVTKTESI AAATEWVKEC VGDRGLWGLV NNAGIFHSHG
YAEWIKIETY RDTLRVNLIG VIEVTLSMLP LVRKAQGRIV NVSSILGRIA FFGAVYSCSK
YGVEAFSDIL RRELQHFGVK VSMVEPGYFR TAMTDWQKFS EIMKQIWKET PAHIKETYGQ
KFFDAYHDLM KQGLLSCSTN LNLVTDCMEH ALTSVHPRTR YSAGWDAQFF FVPLSYLPTS
LADYILTRSW PKPAQAV
