H1FNT_HUMAN
ID H1FNT_HUMAN Reviewed; 255 AA.
AC Q75WM6; Q147U8; Q5GKZ5; Q7Z694;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 3.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Testis-specific H1 histone;
DE AltName: Full=Haploid germ cell-specific nuclear protein 1;
DE AltName: Full=Histone H1.7 {ECO:0000312|HGNC:HGNC:24893};
DE AltName: Full=Histone H1t2;
GN Name=H1-7 {ECO:0000312|HGNC:HGNC:24893};
GN Synonyms=H1FNT {ECO:0000312|HGNC:HGNC:24893}, HANP1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Huang C.Q., Shan Y.X., Wu S.L., Cheng Z.;
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLN-174.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 17-255, FUNCTION, TISSUE SPECIFICITY, AND
RP VARIANTS GLY-84; PRO-108; SER-136; GLN-174 AND PHE-237.
RC TISSUE=Testis;
RX PubMed=16533358; DOI=10.1111/j.1365-2605.2005.00600.x;
RA Tanaka H., Matsuoka Y., Onishi M., Kitamura K., Miyagawa Y., Nishimura H.,
RA Tsujimura A., Okuyama A., Nishimune Y.;
RT "Expression profiles and single-nucleotide polymorphism analysis of human
RT HANP1/H1T2 encoding a histone H1-like protein.";
RL Int. J. Androl. 29:353-359(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-255.
RX PubMed=15710904; DOI=10.1073/pnas.0406060102;
RA Martianov I., Brancorsini S., Catena R., Gansmuller A., Kotaja N.,
RA Parvinen M., Sassone-Corsi P., Davidson I.;
RT "Polar nuclear localization of H1T2, a histone H1 variant, required for
RT spermatid elongation and DNA condensation during spermiogenesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:2808-2813(2005).
CC -!- FUNCTION: Essential for normal spermatogenesis and male fertility
CC (PubMed:16533358). Required for proper cell restructuring and DNA
CC condensation during the elongation phase of spermiogenesis. Involved in
CC the histone-protamine transition of sperm chromatin and the subsequent
CC production of functional sperm. Binds both double-stranded and single-
CC stranded DNA, ATP and protamine-1. {ECO:0000250|UniProtKB:Q8CJI4,
CC ECO:0000269|PubMed:16533358}.
CC -!- INTERACTION:
CC Q75WM6; U3KQK0: H2BC15; NbExp=3; IntAct=EBI-12383084, EBI-12142839;
CC Q75WM6; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-12383084, EBI-16439278;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8CJI4}.
CC Chromosome {ECO:0000250|UniProtKB:Q8CJI4}. Note=In round and elongating
CC spermatids, specifically localizes to a chromatin domain at the apical
CC pole. {ECO:0000250|UniProtKB:Q8CJI4}.
CC -!- TISSUE SPECIFICITY: Testis-specific. {ECO:0000269|PubMed:16533358}.
CC -!- SIMILARITY: Belongs to the histone H1/H5 family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-22 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD13382.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AY302593; AAP60022.1; -; mRNA.
DR EMBL; AC024257; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC118635; AAI18636.1; -; mRNA.
DR EMBL; BC119787; AAI19788.1; -; mRNA.
DR EMBL; AB121028; BAD13382.2; ALT_INIT; mRNA.
DR EMBL; AY496854; AAS49492.1; -; Genomic_DNA.
DR CCDS; CCDS8762.1; -.
DR RefSeq; NP_861453.1; NM_181788.1.
DR AlphaFoldDB; Q75WM6; -.
DR SMR; Q75WM6; -.
DR BioGRID; 131143; 245.
DR IntAct; Q75WM6; 4.
DR STRING; 9606.ENSP00000334805; -.
DR iPTMnet; Q75WM6; -.
DR PhosphoSitePlus; Q75WM6; -.
DR BioMuta; H1FNT; -.
DR DMDM; 215274115; -.
DR MassIVE; Q75WM6; -.
DR PaxDb; Q75WM6; -.
DR PeptideAtlas; Q75WM6; -.
DR PRIDE; Q75WM6; -.
DR ProteomicsDB; 68656; -.
DR Antibodypedia; 55622; 31 antibodies from 13 providers.
DR DNASU; 341567; -.
DR Ensembl; ENST00000335017.1; ENSP00000334805.1; ENSG00000187166.1.
DR GeneID; 341567; -.
DR KEGG; hsa:341567; -.
DR MANE-Select; ENST00000335017.1; ENSP00000334805.1; NM_181788.1; NP_861453.1.
DR UCSC; uc001rrm.3; human.
DR CTD; 341567; -.
DR DisGeNET; 341567; -.
DR GeneCards; H1-7; -.
DR HGNC; HGNC:24893; H1-7.
DR HPA; ENSG00000187166; Tissue enriched (testis).
DR MIM; 618565; gene.
DR neXtProt; NX_Q75WM6; -.
DR OpenTargets; ENSG00000187166; -.
DR VEuPathDB; HostDB:ENSG00000187166; -.
DR eggNOG; ENOG502S7R2; Eukaryota.
DR GeneTree; ENSGT00730000111596; -.
DR HOGENOM; CLU_094439_0_0_1; -.
DR InParanoid; Q75WM6; -.
DR OMA; KEPPCAR; -.
DR OrthoDB; 1487385at2759; -.
DR PhylomeDB; Q75WM6; -.
DR TreeFam; TF338403; -.
DR PathwayCommons; Q75WM6; -.
DR SignaLink; Q75WM6; -.
DR BioGRID-ORCS; 341567; 12 hits in 1071 CRISPR screens.
DR GenomeRNAi; 341567; -.
DR Pharos; Q75WM6; Tdark.
DR PRO; PR:Q75WM6; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q75WM6; protein.
DR Bgee; ENSG00000187166; Expressed in left testis and 95 other tissues.
DR ExpressionAtlas; Q75WM6; baseline and differential.
DR Genevisible; Q75WM6; HS.
DR GO; GO:0000785; C:chromatin; ISS:HGNC.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR GO; GO:0035092; P:sperm DNA condensation; ISS:HGNC.
DR GO; GO:0007290; P:spermatid nucleus elongation; ISS:HGNC.
PE 1: Evidence at protein level;
KW ATP-binding; Chromosome; Developmental protein; Differentiation;
KW DNA condensation; DNA-binding; Nucleotide-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Spermatogenesis.
FT CHAIN 1..255
FT /note="Testis-specific H1 histone"
FT /id="PRO_0000343414"
FT REGION 1..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 124..255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 151..194
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 195..249
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 56
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5RKG3"
FT VARIANT 84
FT /note="R -> G (in dbSNP:rs2732441)"
FT /evidence="ECO:0000269|PubMed:16533358"
FT /id="VAR_044371"
FT VARIANT 108
FT /note="L -> P (in dbSNP:rs766280749)"
FT /evidence="ECO:0000269|PubMed:16533358"
FT /id="VAR_044372"
FT VARIANT 136
FT /note="R -> S (in dbSNP:rs752698412)"
FT /evidence="ECO:0000269|PubMed:16533358"
FT /id="VAR_044373"
FT VARIANT 174
FT /note="R -> Q (in dbSNP:rs1471997)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:16533358"
FT /id="VAR_044374"
FT VARIANT 195
FT /note="R -> Q (in dbSNP:rs1471997)"
FT /id="VAR_047359"
FT VARIANT 237
FT /note="S -> F (in dbSNP:rs2291483)"
FT /evidence="ECO:0000269|PubMed:16533358"
FT /id="VAR_044375"
FT CONFLICT 231
FT /note="K -> N (in Ref. 3; AAI18636)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 255 AA; 28116 MW; F5BA8AB6A4D4E912 CRC64;
MEQALTGEAQ SRWPRRGGSG AMAEAPGPSG ESRGHSATQL PAEKTVGGPS RGCSSSVLRV
SQLVLQAIST HKGLTLAALK KELRNAGYEV RRKSGRHEAP RGQAKATLLR VSGSDAAGYF
RVWKVPKPRR KPGRARQEEG TRAPWRTPAA PRSSRRRRQP LRKAARKARE VWRRNARAKA
KANARARRTR RARPRAKEPP CARAKEEAGA TAADEGRGQA VKEDTTPRSG KDKRRSSKPR
EEKQEPKKPA QRTIQ
