H2A09_CYRHA
ID H2A09_CYRHA Reviewed; 85 AA.
AC D2Y212;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 02-MAR-2010, sequence version 1.
DT 25-MAY-2022, entry version 26.
DE RecName: Full=U4-theraphotoxin-Hhn1a;
DE Short=U4-TRTX-Hhn1a;
DE AltName: Full=Hainantoxin-II.9;
DE Short=HNTX-II.9;
DE AltName: Full=Peptide F8-20.15;
DE Flags: Precursor;
OS Cyriopagopus hainanus (Chinese bird spider) (Haplopelma hainanum).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Mygalomorphae; Theraphosidae; Haplopelma.
OX NCBI_TaxID=209901;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], PROTEIN SEQUENCE OF 49-85, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Venom, and Venom gland;
RX PubMed=20192277; DOI=10.1021/pr1000016;
RA Tang X., Zhang Y., Hu W., Xu D., Tao H., Yang X., Li Y., Jiang L.,
RA Liang S.;
RT "Molecular diversification of peptide toxins from the tarantula Haplopelma
RT hainanum (Ornithoctonus hainana) venom based on transcriptomic, peptidomic,
RT and genomic analyses.";
RL J. Proteome Res. 9:2550-2564(2010).
RN [2]
RP PROTEIN SEQUENCE OF 49-85, FUNCTION, MASS SPECTROMETRY, AND TOXIC DOSE.
RC TISSUE=Venom;
RX PubMed=21174344; DOI=10.3724/sp.j.1141.2010.06570;
RA Pan J.Y., Yu Z.Q.;
RT "Isolation and characterization of Hainantoxin-II, a new neurotoxic peptide
RT from the Chinese bird spider (Haplopelma hainanum).";
RL Dong Wu Xue Yan Jiu 31:570-574(2010).
CC -!- FUNCTION: Neurotoxin active on both insects and mammals.
CC {ECO:0000269|PubMed:21174344}.
CC -!- SUBUNIT: Monomer.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC -!- MASS SPECTROMETRY: Mass=4253.38; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:21174344};
CC -!- TOXIC DOSE: LD(50) is 1.41 mg/kg by intracerebroventricular injection
CC into mice. {ECO:0000269|PubMed:21174344}.
CC -!- TOXIC DOSE: PD(50) is 16 mg/kg in cockroaches.
CC {ECO:0000269|PubMed:21174344}.
CC -!- SIMILARITY: Belongs to the neurotoxin 12 (Hwtx-2) family. 02 (Hwtx-2)
CC subfamily. {ECO:0000305}.
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DR EMBL; GU292889; ADB56705.1; -; mRNA.
DR AlphaFoldDB; D2Y212; -.
DR SMR; D2Y212; -.
DR ArachnoServer; AS001783; U4-theraphotoxin-Hhn1a.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0035792; C:host cell postsynaptic membrane; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR012625; Toxin_20.
DR Pfam; PF08089; Toxin_20; 1.
DR PROSITE; PS60022; HWTX_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Neurotoxin;
KW Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..48
FT /evidence="ECO:0000269|PubMed:20192277,
FT ECO:0000269|PubMed:21174344"
FT /id="PRO_0000400733"
FT PEPTIDE 49..85
FT /note="U4-theraphotoxin-Hhn1a"
FT /id="PRO_0000400734"
FT DISULFID 52..66
FT /evidence="ECO:0000250"
FT DISULFID 56..77
FT /evidence="ECO:0000250"
FT DISULFID 71..82
FT /evidence="ECO:0000250"
SQ SEQUENCE 85 AA; 9417 MW; DF2AD26FF86EE0CB CRC64;
MKVTLIAILT CAAVLVLRTT AAEELEAESQ LMEVGMPDTE LAAVDEERLF ECSVSCEIEK
EGNKDCKKKK CKGGWKCKFN MCVKV