ID   H2A21_CYRHA             Reviewed;          85 AA.
AC   D2Y2K8;
DT   02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   02-MAR-2010, sequence version 1.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=U4-theraphotoxin-Hhn1a;
DE            Short=U4-TRTX-Hhn1a;
DE   AltName: Full=Hainantoxin-II.21;
DE            Short=HNTX-II.21;
DE   AltName: Full=Peptide F8-20.15;
DE   Flags: Precursor;
OS   Cyriopagopus hainanus (Chinese bird spider) (Haplopelma hainanum).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Haplopelma.
OX   NCBI_TaxID=209901;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], PROTEIN SEQUENCE OF 49-85,
RP   AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=20192277; DOI=10.1021/pr1000016;
RA   Tang X., Zhang Y., Hu W., Xu D., Tao H., Yang X., Li Y., Jiang L.,
RA   Liang S.;
RT   "Molecular diversification of peptide toxins from the tarantula Haplopelma
RT   hainanum (Ornithoctonus hainana) venom based on transcriptomic, peptidomic,
RT   and genomic analyses.";
RL   J. Proteome Res. 9:2550-2564(2010).
RN   [2]
RP   PROTEIN SEQUENCE OF 49-85, FUNCTION, MASS SPECTROMETRY, AND TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=21174344; DOI=10.3724/sp.j.1141.2010.06570;
RA   Pan J.Y., Yu Z.Q.;
RT   "Isolation and characterization of Hainantoxin-II, a new neurotoxic peptide
RT   from the Chinese bird spider (Haplopelma hainanum).";
RL   Dong Wu Xue Yan Jiu 31:570-574(2010).
CC   -!- FUNCTION: Neurotoxin active on both insects and mammals.
CC       {ECO:0000269|PubMed:21174344}.
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- MASS SPECTROMETRY: Mass=4253.38; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:21174344};
CC   -!- TOXIC DOSE: LD(50) is 1.41 mg/kg by intracerebroventricular injection
CC       into mice. {ECO:0000269|PubMed:21174344}.
CC   -!- TOXIC DOSE: PD(50) is 16 mg/kg in cockroaches.
CC       {ECO:0000269|PubMed:21174344}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 12 (Hwtx-2) family. 02 (Hwtx-2)
CC       subfamily. {ECO:0000305}.
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DR   EMBL; GU293085; ADB56901.1; -; Genomic_DNA.
DR   AlphaFoldDB; D2Y2K8; -.
DR   SMR; D2Y2K8; -.
DR   ArachnoServer; AS001783; U4-theraphotoxin-Hhn1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0035792; C:host cell postsynaptic membrane; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012625; Toxin_20.
DR   Pfam; PF08089; Toxin_20; 1.
DR   PROSITE; PS60022; HWTX_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..48
FT                   /evidence="ECO:0000269|PubMed:20192277,
FT                   ECO:0000269|PubMed:21174344"
FT                   /id="PRO_0000400757"
FT   PEPTIDE         49..85
FT                   /note="U4-theraphotoxin-Hhn1a"
FT                   /id="PRO_0000400758"
FT   DISULFID        52..66
FT                   /evidence="ECO:0000250"
FT   DISULFID        56..77
FT                   /evidence="ECO:0000250"
FT   DISULFID        71..82
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   85 AA;  9430 MW;  462AC36A1C8FE52F CRC64;
     MKMTLIAILT CAAVLVLHTT AAEELEAESQ LMEVGMPDTE LAAVDEERLF ECSVSCEIEK
     EGNKDCKKKK CKGGWKCKFN MCVKV