ID   H2A_MELV                Reviewed;         168 AA.
AC   A0A097I1R9;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   07-JAN-2015, sequence version 1.
DT   03-AUG-2022, entry version 31.
DE   RecName: Full=Histone doublet miniH2B-H2A {ECO:0000303|PubMed:34297924};
DE   AltName: Full=Histone miniH2B-H2A fusion protein {ECO:0000303|PubMed:34297924};
DE   AltName: Full=MV-miniH2B-H2A {ECO:0000303|PubMed:34297924};
GN   ORFNames=MEL_149 {ECO:0000312|EMBL:AIT54762.1};
OS   Melbournevirus (MelV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes;
OC   Pimascovirales; Marseilleviridae; Marseillevirus;
OC   unclassified Marseillevirus.
OX   NCBI_TaxID=1560514;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=25275139; DOI=10.1128/jvi.02414-14;
RA   Doutre G., Philippe N., Abergel C., Claverie J.M.;
RT   "Genome analysis of the first Marseilleviridae representative from
RT   Australia indicates that most of its genes contribute to virus fitness.";
RL   J. Virol. 88:14340-14349(2014).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX   PubMed=34297924; DOI=10.1016/j.cell.2021.06.032;
RA   Liu Y., Bisio H., Toner C.M., Jeudy S., Philippe N., Zhou K., Bowerman S.,
RA   White A., Edwards G., Abergel C., Luger K.;
RT   "Virus-encoded histone doublets are essential and form nucleosome-like
RT   structures.";
RL   Cell 184:4237-4250.e19(2021).
CC   -!- FUNCTION: Histone-like protein that is recruited to viral factories
CC       during viral replication and participates in viral DNA packaging and
CC       virion production probably by forming unstable nucleosome-like
CC       particles (PubMed:34297924). May compact the viral DNA
CC       (PubMed:34297924). {ECO:0000269|PubMed:34297924}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:34297924}. Host
CC       cytoplasm {ECO:0000269|PubMed:34297924}. Virion
CC       {ECO:0000269|PubMed:34297924}. Note=Localize to cytoplasmic viral
CC       factories after virus infection (PubMed:34297924). Also present in the
CC       nucleus but at much lower concentration (PubMed:34297924). The viral
CC       histones that localize in the nucleus apparently do not interact with
CC       host genomic DNA and can leave the nucleus to associate with the viral
CC       factory (PubMed:34297924). {ECO:0000269|PubMed:34297924}.
CC   -!- INDUCTION: Expression of viral histones begins 2-4 hpi and continues
CC       along the infectious cycle. {ECO:0000269|PubMed:34297924}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; KM275475; AIT54762.1; -; Genomic_DNA.
DR   RefSeq; YP_009094650.1; NC_025412.1.
DR   GeneID; 21012170; -.
DR   KEGG; vg:21012170; -.
DR   Proteomes; UP000207668; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.20.10; -; 1.
DR   InterPro; IPR009072; Histone-fold.
DR   InterPro; IPR007125; Histone_H2A/H2B/H3.
DR   Pfam; PF00125; Histone; 1.
DR   SUPFAM; SSF47113; SSF47113; 1.
PE   2: Evidence at transcript level;
KW   DNA condensation; Host cytoplasm; Host nucleus; Virion.
FT   CHAIN           1..168
FT                   /note="Histone doublet miniH2B-H2A"
FT                   /id="PRO_0000454839"
SQ   SEQUENCE   168 AA;  19137 MW;  DAA8F5E230C146DF CRC64;
     MDRVGKYGLF IKRISPKDAD ITKESLETVN NMLVFLAEKL TKQANIIIDQ KTLRHDAFLW
     LLTDIQGELG KHSQDFANSV LYGEKELVFP TKRTENLMRK NTCLRISQSA VKTLTAILEY
     FCGQIMEASF SQAKKSKRKR IRPIDIEAAI SQDKELHSMF GKGVISGR