H2B2_CAEEL
ID H2B2_CAEEL Reviewed; 123 AA.
AC Q27894; Q95NR2; Q9U373;
DT 15-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Histone H2B 2;
GN Name=his-4; ORFNames=T10C6.11;
GN and
GN Name=his-8; ORFNames=F45F2.12;
GN and
GN Name=his-20; ORFNames=K06C4.4;
GN and
GN Name=his-22; ORFNames=K06C4.12;
GN and
GN Name=his-52; ORFNames=F07B7.4;
GN and
GN Name=his-54; ORFNames=F07B7.11;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP PROTEIN SEQUENCE OF 2-123.
RX PubMed=3753445; DOI=10.1042/bj2350769;
RA Vanfleteren J.R., van Bun S.M., Delcambe L.L., van Beeumen J.J.;
RT "Multiple forms of histone H2B from the nematode Caenorhabditis elegans.";
RL Biochem. J. 235:769-773(1986).
CC -!- FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact
CC DNA into chromatin, limiting DNA accessibility to the cellular
CC machineries which require DNA as a template. Histones thereby play a
CC central role in transcription regulation, DNA repair, DNA replication
CC and chromosomal stability. DNA accessibility is regulated via a complex
CC set of post-translational modifications of histones, also called
CC histone code, and nucleosome remodeling.
CC -!- SUBUNIT: The nucleosome is a histone octamer containing two molecules
CC each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and
CC two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of
CC DNA.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome.
CC -!- PTM: Monoubiquitination of Lys-118 gives a specific tag for epigenetic
CC transcriptional activation and is also prerequisite for histone H3
CC 'Lys-4' and 'Lys-79' methylation. {ECO:0000250}.
CC -!- PTM: GlcNAcylation at Ser-110 promotes monoubiquitination of Lys-118.
CC It fluctuates in response to extracellular glucose, and associates with
CC transcribed genes (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the histone H2B family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CCD68873.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; FO081059; CCD68867.2; -; Genomic_DNA.
DR EMBL; FO081059; CCD68873.1; ALT_INIT; Genomic_DNA.
DR EMBL; FO081551; CCD72362.1; -; Genomic_DNA.
DR EMBL; FO081551; CCD72372.1; -; Genomic_DNA.
DR EMBL; FO081223; CCD70026.1; -; Genomic_DNA.
DR EMBL; Z93388; CAB07654.2; -; Genomic_DNA.
DR PIR; T24788; T24788.
DR PIR; T30054; HSKW22.
DR RefSeq; NP_505197.1; NM_072796.1.
DR RefSeq; NP_505278.2; NM_072877.2.
DR RefSeq; NP_505294.1; NM_072893.1.
DR RefSeq; NP_505295.1; NM_072894.1.
DR RefSeq; NP_507031.2; NM_074630.3.
DR PDB; 6K0C; X-ray; 2.28 A; D=31-123.
DR PDBsum; 6K0C; -.
DR AlphaFoldDB; Q27894; -.
DR SMR; Q27894; -.
DR BioGRID; 44300; 1.
DR BioGRID; 44304; 1.
DR BioGRID; 45063; 4.
DR BioGRID; 48916; 1.
DR BioGRID; 56171; 2.
DR BioGRID; 56175; 1.
DR DIP; DIP-26826N; -.
DR STRING; 6239.F07B7.11; -.
DR EPD; Q27894; -.
DR PaxDb; Q27894; -.
DR PeptideAtlas; Q27894; -.
DR PRIDE; Q27894; -.
DR EnsemblMetazoa; F07B7.11.1; F07B7.11.1; WBGene00001928.
DR EnsemblMetazoa; F07B7.4.1; F07B7.4.1; WBGene00001926.
DR EnsemblMetazoa; F45F2.12.1; F45F2.12.1; WBGene00001882.
DR EnsemblMetazoa; K06C4.12.1; K06C4.12.1; WBGene00001896.
DR EnsemblMetazoa; K06C4.4.1; K06C4.4.1; WBGene00001894.
DR EnsemblMetazoa; T10C6.11.1; T10C6.11.1; WBGene00001878.
DR GeneID; 179262; -.
DR GeneID; 179266; -.
DR GeneID; 180072; -.
DR GeneID; 191670; -.
DR GeneID; 191675; -.
DR KEGG; cel:CELE_F07B7.4; -.
DR KEGG; cel:CELE_F45F2.12; -.
DR KEGG; cel:CELE_K06C4.12; -.
DR KEGG; cel:CELE_K06C4.4; -.
DR KEGG; cel:CELE_T10C6.11; -.
DR UCSC; F45F2.12; c. elegans.
DR CTD; 179262; -.
DR CTD; 179266; -.
DR CTD; 180072; -.
DR CTD; 191670; -.
DR CTD; 191675; -.
DR WormBase; F07B7.11; CE10538; WBGene00001928; his-54.
DR WormBase; F07B7.4; CE10538; WBGene00001926; his-52.
DR WormBase; F45F2.12; CE10538; WBGene00001882; his-8.
DR WormBase; K06C4.12; CE10538; WBGene00001896; his-22.
DR WormBase; K06C4.4; CE10538; WBGene00001894; his-20.
DR WormBase; T10C6.11; CE10538; WBGene00001878; his-4.
DR eggNOG; KOG1744; Eukaryota.
DR GeneTree; ENSGT01050000244943; -.
DR HOGENOM; CLU_075666_2_1_1; -.
DR InParanoid; Q27894; -.
DR OMA; KYTSSRE; -.
DR OrthoDB; 1536672at2759; -.
DR PhylomeDB; Q27894; -.
DR Reactome; R-CEL-201722; Formation of the beta-catenin:TCF transactivating complex.
DR Reactome; R-CEL-212300; PRC2 methylates histones and DNA.
DR Reactome; R-CEL-2299718; Condensation of Prophase Chromosomes.
DR Reactome; R-CEL-2559580; Oxidative Stress Induced Senescence.
DR Reactome; R-CEL-3214815; HDACs deacetylate histones.
DR Reactome; R-CEL-3214847; HATs acetylate histones.
DR Reactome; R-CEL-427359; SIRT1 negatively regulates rRNA expression.
DR Reactome; R-CEL-427413; NoRC negatively regulates rRNA expression.
DR Reactome; R-CEL-5578749; Transcriptional regulation by small RNAs.
DR Reactome; R-CEL-5625886; Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3.
DR Reactome; R-CEL-5689880; Ub-specific processing proteases.
DR Reactome; R-CEL-5693565; Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
DR Reactome; R-CEL-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-CEL-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-CEL-8936459; RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function.
DR Reactome; R-CEL-9018519; Estrogen-dependent gene expression.
DR PRO; PR:Q27894; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00001878; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0044877; F:protein-containing complex binding; ISS:UniProtKB.
DR GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR GO; GO:0006334; P:nucleosome assembly; IBA:GO_Central.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR007125; Histone_H2A/H2B/H3.
DR InterPro; IPR000558; Histone_H2B.
DR PANTHER; PTHR23428; PTHR23428; 1.
DR Pfam; PF00125; Histone; 1.
DR PRINTS; PR00621; HISTONEH2B.
DR SMART; SM00427; H2B; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
DR PROSITE; PS00357; HISTONE_H2B; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chromosome; Direct protein sequencing; DNA-binding;
KW Glycoprotein; Isopeptide bond; Nucleosome core; Nucleus;
KW Reference proteome; Ubl conjugation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:3753445"
FT CHAIN 2..123
FT /note="Histone H2B 2"
FT /id="PRO_0000071868"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 110
FT /note="O-linked (GlcNAc) serine"
FT /evidence="ECO:0000250"
FT CROSSLNK 118
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250"
FT CONFLICT 28..29
FT /note="RH -> KK (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 36..46
FT /evidence="ECO:0007829|PDB:6K0C"
FT HELIX 54..79
FT /evidence="ECO:0007829|PDB:6K0C"
FT HELIX 89..99
FT /evidence="ECO:0007829|PDB:6K0C"
FT HELIX 103..120
FT /evidence="ECO:0007829|PDB:6K0C"
SQ SEQUENCE 123 AA; 13572 MW; A1260A21A632940D CRC64;
MAPPKPSAKG AKKAAKTVTK PKDGKKRRHA RKESYSVYIY RVLKQVHPDT GVSSKAMSIM
NSFVNDVFER IAAEASRLAH YNKRSTISSR EIQTAVRLIL PGELAKHAVS EGTKAVTKYT
SSK
