ID   AMYG_BACLI              Reviewed;          10 AA.
AC   C0HJE2;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2013, sequence version 1.
DT   11-DEC-2019, entry version 5.
DE   RecName: Full=Glucoamylase {ECO:0000303|Ref.1};
DE            EC=3.2.1.3 {ECO:0000269|Ref.1};
DE   AltName: Full=1,4-alpha-D-glucan glucohydrolase {ECO:0000250|UniProtKB:P29761};
DE   AltName: Full=Glucan 1,4-alpha-glucosidase {ECO:0000250|UniProtKB:P29761};
DE   Flags: Fragment;
OS   Bacillus licheniformis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1402;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=KIBGE-IB3 {ECO:0000269|Ref.1};
RA   Ghani M., Rehman H.U., Siddiqui N.N., Aman A., Qader S.A.;
RT   "Isolation, purification and characterization of glucoamylase from wild
RT   strain of Bacillus licheniformis KIBGE-IB3.";
RL   Submitted (AUG-2013) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues
CC         successively from non-reducing ends of the chains with release of
CC         beta-D-glucose.; EC=3.2.1.3; Evidence={ECO:0000269|Ref.1};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         Note=Because of the indeterminate molecular weight of starch, an
CC         approximate KM value of 0.2178 mg/ml was determined.
CC         {ECO:0000269|Ref.1};
CC       pH dependence:
CC         Optimum pH is 5.0. {ECO:0000269|Ref.1};
CC       Temperature dependence:
CC         Optimum temperature is 50 degrees Celsius. {ECO:0000269|Ref.1};
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is: 5.0,
CC       its MW is: 185 kDa. {ECO:0000269|Ref.1}.
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DR   GO; GO:0004339; F:glucan 1,4-alpha-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Direct protein sequencing; Glycosidase; Hydrolase;
KW   Polysaccharide degradation.
FT   CHAIN           1..>10
FT                   /note="Glucoamylase"
FT                   /id="PRO_0000424174"
FT   NON_TER         10
FT                   /evidence="ECO:0000303|Ref.1"
SQ   SEQUENCE   10 AA;  1080 MW;  18FABAA1B1A72334 CRC64;
     SSNKLTTSWG