H2BS2_LYTPI
ID H2BS2_LYTPI Reviewed; 143 AA.
AC P06146;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Histone H2B.2, sperm;
OS Lytechinus pictus (Painted sea urchin).
OC Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC Euechinoidea; Echinacea; Temnopleuroida; Toxopneustidae; Lytechinus.
OX NCBI_TaxID=7653;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Testis;
RX PubMed=3763394; DOI=10.1093/nar/14.17.6845;
RA Lai Z.-C., Childs G.J.;
RT "Isolation and characterization of the gene encoding the testis specific
RT histone protein H2B-2 from the sea urchin Lytechinus pictus.";
RL Nucleic Acids Res. 14:6845-6856(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 59-143, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=3785204; DOI=10.1128/mcb.6.7.2602-2612.1986;
RA Lieber T., Weisser K., Childs G.;
RT "Analysis of histone gene expression in adult tissues of the sea urchins
RT Strongylocentrotus purpuratus and Lytechinus pictus: tissue-specific
RT expression of sperm histone genes.";
RL Mol. Cell. Biol. 6:2602-2612(1986).
CC -!- FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact
CC DNA into chromatin, limiting DNA accessibility to the cellular
CC machineries which require DNA as a template. Histones thereby play a
CC central role in transcription regulation, DNA repair, DNA replication
CC and chromosomal stability. DNA accessibility is regulated via a complex
CC set of post-translational modifications of histones, also called
CC histone code, and nucleosome remodeling.
CC -!- SUBUNIT: The nucleosome is a histone octamer containing two molecules
CC each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and
CC two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of
CC DNA.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome.
CC -!- TISSUE SPECIFICITY: Testis-specific. {ECO:0000269|PubMed:3785204}.
CC -!- DOMAIN: Contains 5 SPKK motifs which may interact with the minor groove
CC of A/T-rich DNA sites. Phosphorylation of this motif may regulate DNA
CC binding. This motif is reiterated in both termini of histone H1 and in
CC the C-terminus of plant H2A, but its presence in the N-terminus seems
CC to be unique to sea urchin histones H2B.
CC -!- PTM: Monoubiquitination of Lys-138 gives a specific tag for epigenetic
CC transcriptional activation and is also prerequisite for histone H3
CC 'Lys-4' and 'Lys-79' methylation. {ECO:0000250}.
CC -!- PTM: Phosphorylated on SPKK motifs 3, 4 and 5; which may regulate DNA
CC binding. Dephosphorylated during maturation of spermatids to mature
CC sperm and rephosphorylated at fertilization (By similarity).
CC {ECO:0000250}.
CC -!- PTM: GlcNAcylation at Ser-130 promotes monoubiquitination of Lys-138.
CC It fluctuates in response to extracellular glucose, and associates with
CC transcribed genes (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the histone H2B family. {ECO:0000305}.
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DR EMBL; X04384; CAA27971.1; -; Genomic_DNA.
DR EMBL; M13635; AAA30001.1; -; mRNA.
DR PIR; A24329; HSURB2.
DR AlphaFoldDB; P06146; -.
DR SMR; P06146; -.
DR iPTMnet; P06146; -.
DR GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR007125; Histone_H2A/H2B/H3.
DR InterPro; IPR000558; Histone_H2B.
DR PANTHER; PTHR23428; PTHR23428; 1.
DR Pfam; PF00125; Histone; 1.
DR PRINTS; PR00621; HISTONEH2B.
DR SMART; SM00427; H2B; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
DR PROSITE; PS00357; HISTONE_H2B; 1.
PE 2: Evidence at transcript level;
KW Chromosome; DNA-binding; Glycoprotein; Isopeptide bond; Nucleosome core;
KW Nucleus; Phosphoprotein; Ubl conjugation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..143
FT /note="Histone H2B.2, sperm"
FT /id="PRO_0000071886"
FT REGION 1..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 4..7
FT /note="SPKK motif 1"
FT MOTIF 9..12
FT /note="SPKK motif 2"
FT MOTIF 14..17
FT /note="SPKK motif 3"
FT MOTIF 19..22
FT /note="SPKK motif 4"
FT MOTIF 24..27
FT /note="SPKK motif 5"
FT COMPBIAS 14..52
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 14
FT /note="Phosphoserine"
FT /evidence="ECO:0000250"
FT MOD_RES 19
FT /note="Phosphoserine"
FT /evidence="ECO:0000250"
FT MOD_RES 24
FT /note="Phosphoserine"
FT /evidence="ECO:0000250"
FT CARBOHYD 130
FT /note="O-linked (GlcNAc) serine"
FT /evidence="ECO:0000250"
FT CROSSLNK 138
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250"
FT VARIANT 76
FT /note="G -> A"
FT VARIANT 138
FT /note="K -> N"
SQ SEQUENCE 143 AA; 15936 MW; D8454E2C2059695D CRC64;
MPRSPSKSSP RKGSPRKGSP RKGSPKRGGK GAKRAGKGGR RRNVVKRRRR RRESYGSYIY
RVLKQVHPDT GISSRGMSVM NSFVNDVFER IAGEASRLCQ ANRRRTISSR EIQTAVRLLL
PGELAKHAVS EGTKAVTKYT TSR
