AMYG_YEAST
ID AMYG_YEAST Reviewed; 549 AA.
AC P08019; D6VVI8; Q07070;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 178.
DE RecName: Full=Glucoamylase, intracellular sporulation-specific;
DE EC=3.2.1.3;
DE AltName: Full=1,4-alpha-D-glucan glucohydrolase;
DE AltName: Full=Glucan 1,4-alpha-glucosidase;
GN Name=SGA1; Synonyms=SGA; OrderedLocusNames=YIL099W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3106330; DOI=10.1128/jb.169.5.2142-2149.1987;
RA Yamashita I., Nakamura M., Fukui S.;
RT "Gene fusion is a possible mechanism underlying the evolution of STA1.";
RL J. Bacteriol. 169:2142-2149(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190.
RC STRAIN=SPX101-1C;
RX PubMed=3141213; DOI=10.1016/0014-5793(88)80912-8;
RA Pardo J.M., Ianez E., Zalacain M., Claros M.G., Jimenez A.;
RT "Similar short elements in the 5' regions of the STA2 and SGA genes from
RT Saccharomyces cerevisiae.";
RL FEBS Lett. 239:179-184(1988).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues
CC successively from non-reducing ends of the chains with release of
CC beta-D-glucose.; EC=3.2.1.3;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 15 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z38125; CAA86282.1; -; Genomic_DNA.
DR EMBL; M16166; AAA35042.1; -; Genomic_DNA.
DR EMBL; X13858; CAA32071.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08454.1; -; Genomic_DNA.
DR PIR; S48474; S48474.
DR RefSeq; NP_012167.3; NM_001179447.3.
DR AlphaFoldDB; P08019; -.
DR SMR; P08019; -.
DR BioGRID; 34892; 28.
DR IntAct; P08019; 1.
DR MINT; P08019; -.
DR STRING; 4932.YIL099W; -.
DR BindingDB; P08019; -.
DR ChEMBL; CHEMBL1075248; -.
DR CAZy; GH15; Glycoside Hydrolase Family 15.
DR PaxDb; P08019; -.
DR PRIDE; P08019; -.
DR EnsemblFungi; YIL099W_mRNA; YIL099W; YIL099W.
DR GeneID; 854708; -.
DR KEGG; sce:YIL099W; -.
DR SGD; S000001361; SGA1.
DR VEuPathDB; FungiDB:YIL099W; -.
DR eggNOG; ENOG502QPM2; Eukaryota.
DR HOGENOM; CLU_012173_2_0_1; -.
DR InParanoid; P08019; -.
DR OMA; DLTWSYG; -.
DR BioCyc; MetaCyc:YIL099W-MON; -.
DR BioCyc; YEAST:YIL099W-MON; -.
DR PRO; PR:P08019; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P08019; protein.
DR GO; GO:0000324; C:fungal-type vacuole; IDA:SGD.
DR GO; GO:0005628; C:prospore membrane; HDA:SGD.
DR GO; GO:0004339; F:glucan 1,4-alpha-glucosidase activity; IDA:SGD.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IBA:GO_Central.
DR GO; GO:0005980; P:glycogen catabolic process; IMP:SGD.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR011613; GH15-like.
DR InterPro; IPR000165; Glucoamylase.
DR Pfam; PF00723; Glyco_hydro_15; 1.
DR PRINTS; PR00736; GLHYDRLASE15.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS00820; GLUCOAMYLASE; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Sporulation.
FT CHAIN 1..549
FT /note="Glucoamylase, intracellular sporulation-specific"
FT /id="PRO_0000186120"
FT ACT_SITE 261
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10051"
FT ACT_SITE 264
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10051"
FT BINDING 198
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CONFLICT 184
FT /note="D -> H (in Ref. 4; CAA32071)"
FT /evidence="ECO:0000305"
FT CONFLICT 504..549
FT /note="HVGTDGELSEQFNKYTGFMQGAQHLTWSYTSFWDAYQIRQEVLQSL -> TW
FT EQTGN (in Ref. 1; AAA35042)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 549 AA; 61463 MW; 6351E84F2CF4AB77 CRC64;
MARQKMFYNK LLGMLSVGFG FAWALENITI YEFDFGKGIL DQSYGGVFSN NGPSQVQLRD
AVLMNGTVVY DSNGAWDSSA LEEWLQGQKK VSIEKIFENI GPSAVYPSIS PGVVIASPSQ
THPDYFYQWI RDSALTINSI VSHSAGPAIE TLLQYLNVSF HLQRSNNTLG AGIGYTNDTV
ALGDPKWNVD NTAFTEDWGR PQNDGPALRS IAILKIIDYI KQSGTDLGAK YPFQSTADIF
DDIVRWDLRF IIDHWNSSGF DLWEEVNGMH FFTLLVQLSA VDKSLSYFNA SERSSPFVEE
LRQTRRDISK FLVDPANGFI NGKYNYIVGT PMIADTLRSG LDISTLLAAN TVHDAPSASH
LPFDINDPAV LNTLHHLMLH MRSIYPINDS SKNATGIALG RYPEDVYDGY GFGEGNPWVL
ATCTASTTLY QLIYRHISEQ HDLVVPMNND CSNAFWSELV FSNLTTLGND EGYLILEFNT
PAFNQTIQKI FQLADSFLVK LKAHVGTDGE LSEQFNKYTG FMQGAQHLTW SYTSFWDAYQ
IRQEVLQSL
