ID   H2_VACCC                Reviewed;         189 AA.
AC   P20496;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Protein H2;
GN   ORFNames=H2R;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Envelope protein part of the entry-fusion complex responsible
CC       for the virus membrane fusion with host cell membrane during virus
CC       entry. Also plays a role in cell-cell fusion (syncytium formation) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of a stable entry-fusion complex (EFC) which is at least
CC       composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. Formation
CC       of the viral membrane is necessary for the assembly of the complex (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC       III membrane protein {ECO:0000305}. Note=Component of the mature virion
CC       (MV) membrane. The mature virion is located in the cytoplasm of
CC       infected cells and is probably released by cell lysis. {ECO:0000250}.
CC   -!- PTM: Contains two intramolecular disulfide bonds. They are created by
CC       the viral disulfide bond formation pathway, a poxvirus-specific pathway
CC       that operates on the cytoplasmic side of the MV membranes (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae H2 family. {ECO:0000305}.
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DR   EMBL; M35027; AAA48089.1; -; Genomic_DNA.
DR   PIR; B42514; B42514.
DR   SMR; P20496; -.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR005023; Pox_LP_H2.
DR   Pfam; PF03356; Pox_LP_H2; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Fusion of virus membrane with host cell membrane;
KW   Fusion of virus membrane with host membrane; Late protein; Membrane;
KW   Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix;
KW   Viral envelope protein; Viral penetration into host cytoplasm; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..189
FT                   /note="Protein H2"
FT                   /id="PRO_0000099547"
FT   TOPO_DOM        1..28
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        29..49
FT                   /note="Helical; Signal-anchor for type III membrane
FT                   protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        50..189
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   189 AA;  21515 MW;  90DDD4564E9A13B2 CRC64;
     MDKTTLSVNA CNLEYVREKA IVGVQAAKTS TLIFFVIILA ISALLLWFQT SDNPVFNELT
     RYMRIKNTVN DWKSLTDSKT KLESDRGKLL AAGKDDIFEF KCVDFGAYFI AMRLDKKTYL
     PQAIRRGTGD AWMVKKAAKV DPSAQQFCQY LIKHKSNNVI TCGNEMLNEL GYSGYFMSPH
     WCSDFSNME